Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACLKM2_RS12095 Genome accession   NZ_CP183899
Coordinates   2542769..2543998 (+) Length   409 a.a.
NCBI ID   WP_159138537.1    Uniprot ID   -
Organism   Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2537769..2548998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM2_RS12050 rimP 2538021..2538545 (-) 525 WP_005094386.1 ribosome maturation factor RimP -
  ACLKM2_RS12080 secG 2539613..2539942 (-) 330 WP_005094388.1 preprotein translocase subunit SecG -
  ACLKM2_RS12085 tpiA 2539956..2540747 (-) 792 WP_411686334.1 triose-phosphate isomerase -
  ACLKM2_RS12090 pilB 2541025..2542737 (+) 1713 WP_005094394.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLKM2_RS12095 pilC 2542769..2543998 (+) 1230 WP_159138537.1 type II secretion system F family protein Machinery gene
  ACLKM2_RS12100 pilD 2543998..2544858 (+) 861 WP_411686335.1 prepilin peptidase Machinery gene
  ACLKM2_RS12105 coaE 2544860..2545459 (+) 600 WP_100329415.1 dephospho-CoA kinase -
  ACLKM2_RS12110 - 2545482..2546405 (-) 924 WP_005094400.1 DMT family transporter -
  ACLKM2_RS12115 rlmB 2546412..2547161 (-) 750 WP_005168959.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACLKM2_RS12120 - 2547298..2547621 (-) 324 WP_004281089.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44950.50 Da        Isoelectric Point: 9.9407

>NTDB_id=1107062 ACLKM2_RS12095 WP_159138537.1 2542769..2543998(+) (pilC) [Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)]
MAAVKKGQMMPIFSYEGIDRKGVKIKGELPARNMALAKVTLRKQGISIKTIREKKKNILEGLMKKRVSTLDITIFTRQLA
TMMKAGVPLVQGFEIVAEGLENPAMREVVLGIKGEVEGGNTFAGALRKYPQYFDKLFCSLVESGEQSGALETMLDRVAIY
KEKSELLKQKIKKAMKYPAAVVVVALIVTIILMVKVVPVFQDLFSSFGADLPAFTKMVVNMSEWMQKYWFLLIIAIGAII
TAFLEAKKRSKKFRDFLDKAALKAPIFGDLVYKAIIARYSRTLATTFAAGVPLIDALESTAGATNNVVYEDAVMKIREDV
ATGQQLQFAMRVTNKFPSMAVQMVAIGEESGALDAMLDKVATHYENEVDNAVDGLTSMMEPLIMAVLGVLVGGLVIAMYL
PIFQMGSVV

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=1107062 ACLKM2_RS12095 WP_159138537.1 2542769..2543998(+) (pilC) [Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)]
ATGGCAGCAGTAAAGAAAGGCCAGATGATGCCGATCTTCAGCTATGAAGGAATTGATCGTAAAGGGGTAAAGATCAAGGG
GGAATTGCCGGCGCGCAACATGGCACTGGCCAAAGTGACGCTCAGAAAACAGGGCATCAGCATCAAGACCATTCGGGAAA
AGAAAAAAAACATTCTCGAAGGCTTAATGAAAAAACGTGTCAGCACACTGGATATTACGATTTTTACTCGACAGCTCGCG
ACCATGATGAAAGCCGGCGTACCTTTAGTACAAGGCTTTGAAATTGTTGCAGAGGGTCTGGAGAATCCAGCCATGCGTGA
AGTCGTTCTGGGGATTAAAGGTGAGGTGGAAGGTGGTAATACCTTTGCCGGTGCACTGCGTAAATACCCGCAATACTTCG
ATAAACTATTTTGTTCACTGGTCGAATCGGGTGAACAATCCGGTGCGCTAGAAACCATGCTGGATCGGGTCGCGATCTAC
AAGGAAAAAAGTGAACTGCTCAAGCAAAAAATTAAAAAAGCCATGAAGTACCCGGCTGCGGTGGTCGTGGTCGCGCTGAT
TGTCACGATTATTCTAATGGTAAAAGTGGTCCCGGTTTTCCAGGATTTATTCTCTTCATTTGGTGCTGACTTACCGGCTT
TTACCAAAATGGTGGTCAATATGTCGGAATGGATGCAAAAATATTGGTTCCTGCTCATTATTGCCATTGGGGCCATCATT
ACGGCTTTTCTGGAAGCAAAAAAACGCAGTAAAAAATTCCGCGACTTTCTGGATAAAGCAGCACTTAAAGCACCGATTTT
CGGTGATCTGGTGTATAAAGCGATTATTGCCCGTTATAGCCGTACCTTGGCCACCACTTTTGCAGCCGGGGTACCCTTGA
TTGATGCACTAGAATCTACTGCCGGCGCAACCAATAACGTGGTGTATGAAGACGCCGTCATGAAAATCCGCGAAGATGTT
GCCACTGGTCAGCAATTACAGTTTGCCATGCGGGTGACCAACAAGTTTCCATCTATGGCCGTGCAAATGGTCGCCATTGG
TGAGGAATCCGGTGCGTTAGATGCCATGCTGGATAAAGTTGCTACCCATTATGAAAATGAAGTCGACAATGCCGTAGACG
GTCTCACTTCCATGATGGAGCCCTTGATCATGGCTGTTCTCGGTGTACTTGTCGGTGGTCTGGTGATTGCCATGTACCTT
CCAATCTTCCAAATGGGTTCTGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(280-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Acinetobacter baumannii D1279779

89.681

99.511

0.892
  pilC Acinetobacter baylyi ADP1

83.047

99.511

0.826
  pilC Pseudomonas stutzeri DSM 10701

62.06

97.311

0.604
  pilC Legionella pneumophila strain ERS1305867

53.904

97.066

0.523
  pilG Neisseria gonorrhoeae MS11

45.98

97.311

0.447
  pilG Neisseria meningitidis 44/76-A

45.98

97.311

0.447
  pilC Vibrio cholerae strain A1552

43.873

99.756

0.438
  pilC Vibrio campbellii strain DS40M4

42.065

97.066

0.408
  pilC Thermus thermophilus HB27

37.037

99.022

0.367

Multiple sequence alignment