Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACLKM2_RS12100 Genome accession   NZ_CP183899
Coordinates   2543998..2544858 (+) Length   286 a.a.
NCBI ID   WP_411686335.1    Uniprot ID   -
Organism   Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2538998..2549858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM2_RS12080 secG 2539613..2539942 (-) 330 WP_005094388.1 preprotein translocase subunit SecG -
  ACLKM2_RS12085 tpiA 2539956..2540747 (-) 792 WP_411686334.1 triose-phosphate isomerase -
  ACLKM2_RS12090 pilB 2541025..2542737 (+) 1713 WP_005094394.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLKM2_RS12095 pilC 2542769..2543998 (+) 1230 WP_159138537.1 type II secretion system F family protein Machinery gene
  ACLKM2_RS12100 pilD 2543998..2544858 (+) 861 WP_411686335.1 prepilin peptidase Machinery gene
  ACLKM2_RS12105 coaE 2544860..2545459 (+) 600 WP_100329415.1 dephospho-CoA kinase -
  ACLKM2_RS12110 - 2545482..2546405 (-) 924 WP_005094400.1 DMT family transporter -
  ACLKM2_RS12115 rlmB 2546412..2547161 (-) 750 WP_005168959.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACLKM2_RS12120 - 2547298..2547621 (-) 324 WP_004281089.1 pyrimidine/purine nucleoside phosphorylase -
  ACLKM2_RS12125 recN 2548036..2549697 (+) 1662 WP_180075298.1 DNA repair protein RecN -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32037.25 Da        Isoelectric Point: 7.8044

>NTDB_id=1107063 ACLKM2_RS12100 WP_411686335.1 2543998..2544858(+) (pilD) [Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)]
MQDLIQYFIQNPTALYSAVGLFSLCIGSFLNVVIYRTPKMMEQEWRTDCQMFLHPEQPIIDETKLSLSKPDSTCPKCHQP
IRWYQNIPVISWLVLRGKCGNCQNPISIRYPLIELLTAACALMVVAVFGPTVQMLFGLILTYVLIALTFIDFDTQLLPDR
YTLPLAALGLGVNSYALYTSPSSAIWGYIIGFLCLWVVYYVFKIVTGKEGMGYGDFKLLAALGAWMGPLLLPLIVLLSSL
VGAIIGIILLKVRKENQPFAFGPYIAIAGWIAFLWGEQIMKVYLGQ

Nucleotide


Download         Length: 861 bp        

>NTDB_id=1107063 ACLKM2_RS12100 WP_411686335.1 2543998..2544858(+) (pilD) [Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)]
ATGCAAGATCTTATTCAATATTTCATTCAAAACCCAACAGCGCTGTATAGCGCAGTTGGGCTTTTTAGTCTCTGCATTGG
TAGTTTCTTAAATGTGGTGATTTATCGCACTCCCAAGATGATGGAGCAGGAATGGCGTACCGATTGCCAAATGTTCCTGC
ATCCGGAACAGCCAATTATTGATGAGACCAAATTAAGTTTAAGCAAACCCGATTCGACTTGCCCCAAATGTCATCAGCCA
ATCCGCTGGTACCAGAATATTCCCGTCATCAGCTGGCTGGTCTTGCGCGGCAAGTGCGGCAACTGTCAAAATCCCATCAG
TATCCGCTATCCACTGATTGAGCTTCTGACCGCGGCCTGTGCGCTGATGGTGGTGGCTGTTTTTGGTCCGACTGTCCAGA
TGTTATTTGGCCTGATTCTGACTTATGTCTTAATTGCACTGACCTTTATTGATTTTGACACCCAGTTATTGCCGGACCGT
TATACCCTACCCCTGGCCGCGCTGGGTCTGGGCGTCAATAGTTATGCCCTCTATACCTCACCGAGTTCTGCCATCTGGGG
CTATATCATTGGCTTCCTGTGTTTGTGGGTGGTGTATTATGTCTTTAAAATTGTCACTGGCAAGGAAGGTATGGGCTATG
GCGATTTTAAATTGCTTGCCGCCTTGGGTGCCTGGATGGGGCCTTTGCTATTACCATTAATTGTGCTCCTTTCTTCTTTA
GTGGGTGCGATTATCGGGATTATCCTGCTGAAAGTCCGTAAGGAAAATCAGCCTTTTGCCTTTGGTCCCTATATCGCGAT
TGCCGGCTGGATTGCTTTTCTGTGGGGCGAGCAGATTATGAAAGTGTATTTAGGTCAATAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)

Type IV leader peptidase family (Peptidase_A24)

(139-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

82.105

99.65

0.818
  pilD Acinetobacter baumannii D1279779

81.754

99.65

0.815
  pilD Vibrio cholerae strain A1552

55.805

93.357

0.521
  pilD Vibrio campbellii strain DS40M4

53.184

93.357

0.497
  pilD Neisseria gonorrhoeae MS11

48.864

92.308

0.451

Multiple sequence alignment