Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACN08T_RS03890 Genome accession   NZ_CP183248
Coordinates   797200..798885 (-) Length   561 a.a.
NCBI ID   WP_053988072.1    Uniprot ID   -
Organism   Photobacterium leiognathi subsp. mandapamensis strain Sc9.10a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 792200..803885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN08T_RS03860 (ACN08T_03860) mutT 792916..793320 (-) 405 WP_419205225.1 8-oxo-dGTP diphosphatase MutT -
  ACN08T_RS03865 (ACN08T_03865) yacG 793325..793546 (-) 222 WP_008987916.1 DNA gyrase inhibitor YacG -
  ACN08T_RS03870 (ACN08T_03870) zapD 793554..794300 (-) 747 WP_042117295.1 cell division protein ZapD -
  ACN08T_RS03875 (ACN08T_03875) coaE 794365..794970 (-) 606 WP_419205226.1 dephospho-CoA kinase -
  ACN08T_RS03880 (ACN08T_03880) pilD 794974..795843 (-) 870 WP_419205227.1 prepilin peptidase Machinery gene
  ACN08T_RS03885 (ACN08T_03885) pilC 795932..797155 (-) 1224 WP_305811052.1 type II secretion system F family protein Machinery gene
  ACN08T_RS03890 (ACN08T_03890) pilB 797200..798885 (-) 1686 WP_053988072.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACN08T_RS03895 (ACN08T_03895) pilA 798887..799330 (-) 444 WP_419205228.1 pilin Machinery gene
  ACN08T_RS03900 (ACN08T_03900) nadC 799678..800565 (-) 888 WP_419206120.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACN08T_RS03905 (ACN08T_03905) ampD 800816..801361 (+) 546 WP_419205229.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACN08T_RS03910 (ACN08T_03910) pdhR 801683..802456 (+) 774 WP_008987925.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62097.53 Da        Isoelectric Point: 5.0205

>NTDB_id=1104813 ACN08T_RS03890 WP_053988072.1 797200..798885(-) (pilB) [Photobacterium leiognathi subsp. mandapamensis strain Sc9.10a]
MQTNLASILAQANLISPEQEQQVVEFVTAEGINVPTALTRLGILSDLDLADNLANLFGLQKVDVHHYDYDGCCQSVYQRE
LVLRHRALPLTQDANSLFLGVSDPTNMDAQDEFRFAVGKTVEFLILENKQLESAIRRVYGSDIGETSHSKQVSDADLGEL
VDISDAELDEATDLTQDSAPVTRYINQVLLDAVRRKASDIHFEPYEHNYRIRFRCDGILHQHLTPPASLSRRLSTRLKVM
SKLNIAERRQPQDGRIKLKLSDTLAIDLRVSTLPTLWGEKVVLRILDSSSANLDINILGYNDKQKSDYLTALERPQGMIL
ITGPTGSGKTVSLYTGLNILNTDERNISTAEDPVEINLPGINQVQINQQVGLSFADALRSFLRQDPDIVMVGEIRDLETA
SIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGVEDFNLASSLSLIIAQRLARRLCKSCKHPHPLDEITCQRLNVPLNTT
VYQANNAGCDDCNKGYAGRVGIYEVMVFSREIAEALMANATALQLEDIAVKQGMQRLKDSGVEKLLEGVTSLSELQRVLQ
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1104813 ACN08T_RS03890 WP_053988072.1 797200..798885(-) (pilB) [Photobacterium leiognathi subsp. mandapamensis strain Sc9.10a]
ATGCAAACCAACCTCGCTTCTATCTTGGCTCAAGCTAATTTAATTAGCCCAGAGCAAGAACAACAAGTGGTCGAGTTTGT
GACGGCGGAAGGGATTAACGTCCCGACCGCCTTAACCCGTTTAGGGATATTAAGTGATTTAGATTTAGCCGATAACCTAG
CTAATTTATTTGGCTTACAAAAAGTCGATGTTCACCACTACGACTATGACGGTTGCTGTCAAAGTGTTTACCAACGTGAA
TTAGTATTGCGTCACCGCGCACTCCCATTAACCCAAGATGCTAATAGCCTATTTTTAGGGGTCAGCGATCCCACCAATAT
GGATGCGCAAGACGAATTTCGTTTTGCAGTAGGTAAAACTGTTGAGTTTCTAATTTTAGAAAACAAACAATTAGAATCTG
CCATTCGCCGTGTTTACGGTAGCGATATTGGTGAAACCAGCCACAGCAAACAAGTCAGTGATGCCGATTTAGGCGAGCTG
GTTGATATTAGCGATGCAGAACTTGATGAAGCGACAGATTTAACCCAAGACAGTGCTCCCGTTACCCGTTACATCAACCA
AGTGTTATTAGACGCTGTACGTCGTAAAGCGTCAGATATTCACTTTGAACCCTACGAGCATAACTATCGAATTCGTTTTC
GTTGTGACGGTATTCTGCATCAACACTTAACGCCACCTGCGAGTTTGTCACGCCGTTTATCAACCCGTTTAAAGGTCATG
TCGAAGCTCAACATTGCCGAGCGTCGCCAACCGCAAGACGGACGTATTAAACTAAAGCTATCAGATACTTTAGCAATTGA
TTTACGTGTTTCTACCCTACCAACACTGTGGGGTGAAAAAGTGGTACTGCGGATATTAGATAGCTCTTCAGCGAATCTTG
ATATCAATATTCTCGGTTACAACGATAAGCAAAAATCCGATTATTTAACCGCCCTTGAACGCCCACAAGGGATGATATTA
ATCACAGGCCCAACAGGTAGCGGTAAAACCGTCTCCCTTTATACTGGACTTAATATTTTAAATACCGATGAGCGCAATAT
TTCTACTGCTGAAGATCCGGTGGAAATTAATTTACCCGGTATTAACCAAGTACAGATCAATCAGCAAGTGGGTTTAAGTT
TTGCCGATGCACTGCGTTCATTTCTACGCCAAGACCCCGACATCGTGATGGTCGGTGAGATCCGCGATTTAGAAACCGCC
TCCATTGCGGTAAAAGCCGCCCAAACAGGTCACTTGGTATTATCAACCCTGCATACTAACTCTGCTGCTGAAACCATTAC
CCGTTTAACCAATATGGGGGTGGAAGATTTTAACCTCGCTTCATCGCTGAGCTTAATCATAGCCCAGCGTCTTGCCCGTC
GTTTATGTAAAAGCTGTAAACACCCTCACCCACTGGATGAAATCACTTGCCAGCGTTTAAACGTGCCGCTCAATACCACG
GTTTATCAAGCCAACAATGCAGGTTGCGATGATTGTAATAAAGGCTATGCCGGACGTGTCGGTATTTATGAAGTGATGGT
ATTTAGCCGAGAAATAGCCGAAGCCTTAATGGCGAATGCCACTGCATTACAACTAGAAGATATTGCTGTTAAACAAGGTA
TGCAGCGCTTAAAAGATTCAGGGGTCGAGAAGTTACTTGAAGGCGTAACTAGCCTGTCTGAACTGCAACGTGTGTTACAG
CTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)

Type II/IV secretion system protein (T2SSE)

(187-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

62.857

99.822

0.627
  pilB Vibrio parahaemolyticus RIMD 2210633

59.821

99.822

0.597
  pilB Vibrio campbellii strain DS40M4

59.464

99.822

0.594
  pilB Acinetobacter baumannii D1279779

53.191

92.157

0.49
  pilB Legionella pneumophila strain ERS1305867

48.41

100

0.488
  pilB Acinetobacter baylyi ADP1

51.724

93.048

0.481
  pilF Neisseria gonorrhoeae MS11

46.004

100

0.462
  pilF Thermus thermophilus HB27

38.053

100

0.383
  pilB/pilB1 Synechocystis sp. PCC 6803

40.504

91.979

0.373

Multiple sequence alignment