Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACN08T_RS02900 Genome accession   NZ_CP183248
Coordinates   599903..601612 (+) Length   569 a.a.
NCBI ID   WP_419206118.1    Uniprot ID   -
Organism   Photobacterium leiognathi subsp. mandapamensis strain Sc9.10a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 594903..606612
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN08T_RS02880 (ACN08T_02880) pilM 597155..598138 (+) 984 WP_045068186.1 type IV pilus biogenesis protein PilM -
  ACN08T_RS02885 (ACN08T_02885) - 598135..598737 (+) 603 WP_008988125.1 PilN domain-containing protein -
  ACN08T_RS02890 (ACN08T_02890) - 598738..599352 (+) 615 WP_419205156.1 type 4a pilus biogenesis protein PilO -
  ACN08T_RS02895 (ACN08T_02895) - 599356..599871 (+) 516 WP_008988123.1 pilus assembly protein PilP -
  ACN08T_RS02900 (ACN08T_02900) pilQ 599903..601612 (+) 1710 WP_419206118.1 type IV pilus secretin PilQ Machinery gene
  ACN08T_RS02905 (ACN08T_02905) aroK 602000..602518 (+) 519 WP_008988121.1 shikimate kinase AroK -
  ACN08T_RS02910 (ACN08T_02910) aroB 602542..603621 (+) 1080 WP_318447483.1 3-dehydroquinate synthase -
  ACN08T_RS02915 (ACN08T_02915) - 603635..605251 (+) 1617 WP_419205157.1 AAA family ATPase -
  ACN08T_RS02920 (ACN08T_02920) dam 605359..606210 (+) 852 WP_023933777.1 adenine-specific DNA-methyltransferase -
  ACN08T_RS02925 (ACN08T_02925) - 606211..606420 (-) 210 WP_023933775.1 DUF2970 domain-containing protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62066.28 Da        Isoelectric Point: 5.1992

>NTDB_id=1104808 ACN08T_RS02900 WP_419206118.1 599903..601612(+) (pilQ) [Photobacterium leiognathi subsp. mandapamensis strain Sc9.10a]
MLSRQYLATLWLLCVGLLAFPSVAAVPAAGMIDNQIQAIDFRRDGKGRGVLTVRLEKPQSLVDLRRNGNKLQLDIFDTML
KDDMVYTLDVVDFATVVESIETMRVSKGARLMFNMKGSYDYDYNLRGKRLEVIIEKRAAKKAVSGSKSNAKINYKGKPIS
INFQDIPVRNVLQLIADYNDFNLVVSDSVSGNVTLRLDDVPWPQVLDIILQSKDLDKRVRGSVLLVAPKSELTASEHQVM
EAKRKQQELSNLRSELIQVNYAKATDIAALLSGSTDGVGMLSERGSLHVDERTNSLIIKDISDNISSIKNIVESLDIPVK
QVQIEARIVTINEGDIDELGVRWGVSKVNGDTTIGGSIEGNLASSGLLGEDATVDDFLNVNLGASAGAASIAFQVAKLGN
ILLDLELSALQTENKAEIISSPRLMTTNKKTAYIEQGTEIPYLEASSSGATSVSFKKAVLSLMVTPQITPDDKLVLDLEV
TQDNKGEDVPTGEGTAVSIDTQRIGTQVLVNNGETVVLGGIYQHSMTNVTRKVPVLGDIPVLGALFRHKMEQMGKRELLI
FVTPKIVLQ

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=1104808 ACN08T_RS02900 WP_419206118.1 599903..601612(+) (pilQ) [Photobacterium leiognathi subsp. mandapamensis strain Sc9.10a]
ATGTTAAGCCGCCAGTATCTAGCAACACTTTGGTTGTTGTGTGTTGGATTATTGGCTTTTCCTAGTGTGGCTGCTGTGCC
TGCGGCAGGGATGATAGATAACCAAATTCAAGCCATTGATTTTCGTCGTGATGGTAAAGGGCGTGGTGTCCTAACAGTGC
GTCTGGAAAAGCCACAATCGTTAGTGGATTTGCGTCGTAATGGCAATAAGTTACAGCTAGATATTTTCGATACTATGCTT
AAAGATGACATGGTGTACACACTTGATGTGGTTGATTTTGCAACTGTTGTCGAAAGTATTGAAACCATGCGAGTGTCAAA
AGGCGCGCGCCTCATGTTCAACATGAAAGGCAGCTACGATTATGATTACAATCTACGTGGTAAGCGCTTAGAAGTGATCA
TTGAAAAGCGTGCAGCGAAGAAGGCCGTATCAGGTAGTAAATCAAATGCCAAAATTAATTATAAAGGCAAGCCGATTTCA
ATTAACTTCCAAGATATCCCGGTACGTAACGTTCTACAGCTAATTGCTGATTACAACGACTTCAACTTGGTCGTGTCTGA
TTCCGTTAGTGGTAATGTGACATTGCGTTTAGACGATGTGCCATGGCCACAAGTGCTAGATATTATTCTACAGTCAAAAG
ACTTAGATAAACGTGTAAGAGGCTCTGTGTTGCTGGTTGCACCTAAATCTGAGCTAACAGCCAGTGAGCATCAGGTAATG
GAAGCAAAGCGTAAGCAGCAAGAGCTATCAAATCTGCGCTCAGAGCTTATCCAAGTAAACTACGCAAAAGCGACGGATAT
TGCTGCGCTGCTAAGTGGCAGTACAGATGGTGTTGGTATGCTGTCTGAACGTGGCTCACTGCATGTCGATGAGCGTACTA
ACTCGCTTATCATCAAAGATATCTCTGATAATATCTCAAGTATTAAAAACATTGTTGAGTCATTAGATATCCCGGTTAAA
CAGGTTCAGATTGAAGCGCGTATCGTGACTATCAATGAAGGCGATATTGATGAGTTAGGTGTTCGCTGGGGGGTATCGAA
AGTTAATGGTGATACCACCATTGGTGGTTCAATTGAAGGTAACTTGGCAAGCTCAGGTCTGTTAGGTGAAGACGCAACGG
TGGATGATTTCCTAAACGTCAACTTAGGTGCAAGCGCAGGTGCGGCGTCCATTGCTTTCCAAGTGGCGAAGCTGGGTAAT
ATCTTATTAGATTTAGAATTATCTGCTCTGCAAACCGAAAACAAAGCCGAAATTATCTCAAGCCCACGTTTGATGACCAC
CAATAAGAAAACTGCTTACATCGAGCAAGGTACAGAAATTCCTTACTTAGAAGCCTCATCTAGTGGTGCGACATCGGTAT
CATTTAAAAAGGCCGTGCTAAGTTTGATGGTGACACCACAAATCACTCCTGATGATAAGTTAGTGTTAGATCTTGAAGTG
ACACAAGATAACAAGGGTGAAGATGTTCCAACCGGTGAAGGTACGGCAGTCTCTATCGATACCCAACGTATTGGTACTCA
GGTGCTAGTTAATAATGGCGAAACCGTTGTACTGGGTGGTATTTACCAGCACAGTATGACCAACGTAACCCGTAAGGTAC
CAGTATTGGGTGATATCCCTGTGCTTGGTGCACTGTTCCGCCATAAAATGGAGCAAATGGGTAAGCGTGAACTGCTTATT
TTCGTGACACCAAAAATTGTTCTTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(255-321)

Bacterial type II and III secretion system protein (Secretin)

(409-567)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

62.281

100

0.624
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

62.281

100

0.624
  pilQ Vibrio campbellii strain DS40M4

61.982

97.54

0.605
  pilQ Pseudomonas aeruginosa PAK

43.75

98.418

0.431
  comQ Acinetobacter baylyi ADP1

40.652

100

0.417
  pilQ Acinetobacter baumannii strain A118

38.786

100

0.404
  pilQ Acinetobacter baumannii D1279779

38.786

100

0.404
  pilQ Legionella pneumophila strain Lp02

41.774

95.079

0.397
  pilQ Legionella pneumophila strain ERS1305867

41.774

95.079

0.397

Multiple sequence alignment