Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACNS8M_RS12435 Genome accession   NZ_CP183239
Coordinates   2614252..2616393 (-) Length   713 a.a.
NCBI ID   WP_419225342.1    Uniprot ID   -
Organism   Acinetobacter sp. A2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2609252..2621393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNS8M_RS12420 (ACNS8M_12355) - 2611709..2612563 (-) 855 WP_419224870.1 hypothetical protein -
  ACNS8M_RS12425 (ACNS8M_12360) aroB 2612573..2613652 (-) 1080 WP_168460758.1 3-dehydroquinate synthase -
  ACNS8M_RS12430 (ACNS8M_12365) aroK 2613672..2614214 (-) 543 WP_096901281.1 shikimate kinase AroK -
  ACNS8M_RS12435 (ACNS8M_12370) pilQ 2614252..2616393 (-) 2142 WP_419225342.1 type IV pilus secretin PilQ Machinery gene
  ACNS8M_RS12440 (ACNS8M_12375) pilP 2616454..2616978 (-) 525 WP_104852650.1 pilus assembly protein PilP Machinery gene
  ACNS8M_RS12445 (ACNS8M_12380) comO 2616978..2617688 (-) 711 WP_419224871.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACNS8M_RS12450 (ACNS8M_12385) comN 2617685..2618320 (-) 636 WP_104852652.1 PilN domain-containing protein Machinery gene
  ACNS8M_RS12455 (ACNS8M_12390) pilM 2618320..2619378 (-) 1059 WP_194559041.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 713 a.a.        Molecular weight: 76756.90 Da        Isoelectric Point: 5.2214

>NTDB_id=1104753 ACNS8M_RS12435 WP_419225342.1 2614252..2616393(-) (pilQ) [Acinetobacter sp. A2]
MNNLFRQFSMGAVAMAVMQVANAQVSITNIVPMQIPGQGTEIRVMFNGLPPQPAAYQLEQPARLILDFDKVQQTLKQNSV
AVATKEASSVEVSSDAQRSRLTVNLADAGAFTTRVEGNTFILKINSTTAATPVAAVTAQRSAQGISNIGFQRGGEGEGQV
VIDLLGSNTPVDVQQQGSKIVVRTLGNKVPAHLARRLNVNDFATPVSTIDAHNDGSNGVITIQAAGSYEYMAYQAEDKLT
ISLKRPVDKNPFKPRAAQSYTGKKISLDFQDIEVRRVLQLLADFTGINMVAADTVQGNITLRLKDVPWDQALDIILKTKN
LDKRRNGNVIWIAPVSELIKAEEEEAKAIAQSVKLAPLQTEYIQLNYAKAADIEKLITEGKNASRSGSSNTGSGADPLGD
SVGSLLSPRGTVSVDPRTNTLIINDTSAKIDQIRQMIDLLDVSVKQVMIEARIVRATTDFTKEMGVKWGILSQGITNNNH
LLVGGSDTTLWNLREPEANSELGGYTYDIERPQNLNVDLGVTSPGASRIAFGLISLSDFMLDLELSALQADGYGEVISTP
KVLTADKQNAKVASGTQIPYQSSEGGGVNAVSTTEFIDATLSLDVTPSITPDGKVQMQLNITSDSPGAPTPTGQLTINKN
AVNTNVLVDNGETVVLGGIFEQQTANSQTKVPFLGDIPYIGRLFRKDIKSDNKRELLIFVTPRIVNDSVSRNH

Nucleotide


Download         Length: 2142 bp        

>NTDB_id=1104753 ACNS8M_RS12435 WP_419225342.1 2614252..2616393(-) (pilQ) [Acinetobacter sp. A2]
ATGAACAATTTGTTCCGTCAGTTTTCAATGGGCGCAGTCGCAATGGCGGTGATGCAAGTGGCTAATGCCCAAGTGAGCAT
TACCAATATTGTACCAATGCAAATTCCAGGACAAGGCACGGAAATTCGTGTCATGTTCAATGGCTTACCACCACAGCCAG
CGGCATATCAGTTGGAACAACCTGCGCGTTTGATTCTAGACTTTGATAAAGTACAGCAAACGTTGAAACAAAACAGTGTT
GCAGTGGCGACCAAAGAAGCCAGTTCTGTAGAGGTCAGCTCAGATGCTCAGCGTTCACGTTTAACTGTGAACTTGGCTGA
TGCAGGTGCGTTTACGACACGTGTTGAAGGCAATACCTTTATTTTAAAGATCAACTCAACTACCGCAGCCACACCTGTTG
CAGCAGTGACTGCACAACGTAGCGCACAAGGAATTTCCAATATTGGTTTCCAACGTGGTGGCGAAGGTGAAGGTCAGGTG
GTAATTGACCTATTGGGTAGCAACACCCCTGTAGATGTACAGCAACAAGGCAGCAAAATTGTCGTACGTACTTTGGGCAA
TAAAGTCCCTGCGCATTTGGCTCGTCGCCTGAATGTGAATGACTTTGCAACCCCAGTATCAACCATTGATGCGCATAATG
ATGGTAGCAATGGTGTGATCACCATTCAGGCTGCAGGTAGCTATGAATATATGGCCTATCAGGCAGAAGACAAGCTCACC
ATCAGCTTAAAACGCCCAGTTGATAAAAACCCGTTTAAGCCACGTGCAGCACAAAGCTACACAGGTAAAAAGATTTCATT
GGACTTCCAAGATATCGAAGTGCGTCGTGTATTACAGCTGTTGGCAGACTTTACAGGCATCAATATGGTGGCTGCAGATA
CAGTACAAGGTAATATCACCTTACGTTTAAAAGATGTGCCTTGGGATCAAGCCTTAGACATTATTCTGAAAACCAAAAAT
TTAGACAAGCGCCGTAATGGTAATGTGATCTGGATTGCGCCAGTTTCTGAGTTGATTAAGGCGGAAGAAGAAGAAGCCAA
AGCGATTGCACAAAGTGTTAAATTGGCACCACTGCAAACTGAATATATTCAGTTGAACTATGCTAAAGCCGCAGATATTG
AGAAGCTTATTACTGAAGGTAAAAATGCATCACGTAGTGGTAGTTCAAATACAGGTTCTGGAGCTGACCCATTAGGTGAT
AGCGTGGGGAGCTTGCTCAGCCCTCGCGGTACAGTGTCTGTAGATCCACGTACCAATACCTTAATTATTAATGATACTTC
AGCAAAAATTGATCAGATTCGTCAGATGATAGATTTGCTTGATGTGTCTGTGAAGCAAGTGATGATTGAAGCACGTATCG
TACGTGCAACCACAGATTTCACCAAAGAGATGGGTGTGAAGTGGGGCATCTTGTCACAAGGGATCACCAACAATAATCAT
TTGTTGGTGGGTGGTAGTGATACCACTTTGTGGAACTTGCGTGAGCCAGAAGCGAATAGTGAGTTAGGCGGCTATACTTA
TGATATTGAGCGTCCGCAAAACCTCAATGTCGATTTAGGTGTGACCAGTCCAGGTGCCAGCCGTATTGCGTTTGGTTTAA
TCAGTTTATCTGACTTTATGTTGGATTTGGAATTGTCTGCATTACAAGCCGATGGCTATGGTGAAGTGATTTCAACACCA
AAAGTACTGACTGCAGATAAGCAAAATGCCAAAGTTGCATCGGGTACTCAAATTCCATACCAATCTTCTGAAGGTGGCGG
TGTTAATGCTGTCTCGACCACAGAATTTATTGATGCGACTTTAAGTTTGGATGTGACGCCAAGTATCACGCCAGATGGCA
AGGTGCAAATGCAATTGAATATCACCAGTGACTCGCCAGGTGCGCCAACACCAACTGGTCAGCTCACCATTAACAAGAAC
GCAGTCAATACCAATGTCTTGGTAGATAATGGCGAAACTGTGGTCTTGGGTGGCATTTTTGAGCAGCAAACTGCCAACTC
ACAAACCAAGGTTCCATTCTTGGGTGATATTCCTTATATCGGACGTTTATTCCGTAAAGATATCAAGTCGGATAACAAAC
GTGAGTTGCTGATTTTTGTTACACCACGAATTGTTAATGACAGTGTTTCAAGAAATCATTAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(40-120)

Bacterial type II/III secretion system short domain (Secretin_N)

(361-446)

Bacterial type II and III secretion system protein (Secretin)

(547-705)

Secretin and TonB N terminus short domain (STN)

(292-334)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Acinetobacter baumannii D1279779

75.967

100

0.771
  pilQ Acinetobacter baumannii strain A118

75.829

100

0.77
  comQ Acinetobacter baylyi ADP1

73.846

100

0.741
  pilQ Pseudomonas aeruginosa PAK

41.369

98.317

0.407
  pilQ Legionella pneumophila strain ERS1305867

37.429

99.299

0.372
  pilQ Legionella pneumophila strain Lp02

37.429

99.299

0.372

Multiple sequence alignment