Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACNI65_RS02495 Genome accession   NZ_CP182288
Coordinates   542827..543273 (-) Length   148 a.a.
NCBI ID   WP_416760975.1    Uniprot ID   -
Organism   Roseateles sp. So40a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 537827..548273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNI65_RS02470 (ACNI65_02470) - 538135..539124 (+) 990 WP_416760970.1 RluA family pseudouridine synthase -
  ACNI65_RS02475 (ACNI65_02475) - 539159..539815 (+) 657 WP_416760971.1 HAD family hydrolase -
  ACNI65_RS02480 (ACNI65_02480) - 539887..540273 (+) 387 WP_416760972.1 Rieske (2Fe-2S) protein -
  ACNI65_RS02485 (ACNI65_02485) - 540274..541326 (+) 1053 WP_416760973.1 S49 family peptidase -
  ACNI65_RS02490 (ACNI65_02490) - 541362..542801 (+) 1440 WP_416760974.1 FAD-binding oxidoreductase -
  ACNI65_RS02495 (ACNI65_02495) pilE 542827..543273 (-) 447 WP_416760975.1 pilin Machinery gene
  ACNI65_RS02500 (ACNI65_02500) - 543547..544389 (+) 843 WP_416760977.1 ChaN family lipoprotein -
  ACNI65_RS02505 (ACNI65_02505) - 544451..545257 (-) 807 WP_416760979.1 DeoR/GlpR family DNA-binding transcription regulator -
  ACNI65_RS02510 (ACNI65_02510) - 545352..546311 (-) 960 WP_416760980.1 N-acetylglucosamine kinase -
  ACNI65_RS02515 (ACNI65_02515) - 546329..547492 (-) 1164 WP_416760981.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15275.59 Da        Isoelectric Point: 9.0011

>NTDB_id=1100988 ACNI65_RS02495 WP_416760975.1 542827..543273(-) (pilE) [Roseateles sp. So40a]
MKRVQQGFTLIELMIVVAIIGILAAVALPAYSDYIKKSKVTEVMGIASLPKAAIQEWLSVKGSFPADEATLGLTSNPTKY
VSSVTYTGGATAVLTATAQNIGTGVNGNTVVLTGTRVNNSSGTATDQVTWACTGSIEAKYLPASCKGT

Nucleotide


Download         Length: 447 bp        

>NTDB_id=1100988 ACNI65_RS02495 WP_416760975.1 542827..543273(-) (pilE) [Roseateles sp. So40a]
ATGAAGCGCGTTCAACAAGGTTTCACGCTGATCGAACTGATGATCGTCGTGGCGATCATCGGCATCCTCGCCGCCGTGGC
CCTGCCGGCCTACTCGGACTACATCAAGAAGTCCAAGGTCACCGAGGTCATGGGCATCGCCAGCCTGCCCAAGGCGGCGA
TCCAGGAATGGCTGTCGGTCAAGGGTTCGTTCCCGGCGGATGAGGCCACGCTGGGCCTGACGTCGAATCCGACCAAGTAC
GTGTCGAGCGTGACCTACACCGGCGGCGCGACGGCCGTGCTGACCGCCACGGCCCAGAACATCGGTACCGGCGTGAACGG
CAACACCGTGGTCCTGACCGGCACGCGGGTGAACAACAGCAGCGGCACGGCCACCGACCAGGTGACCTGGGCCTGCACCG
GCTCGATCGAAGCCAAGTACCTGCCTGCCAGCTGCAAGGGCACCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)

Pilin (bacterial filament) (Pilin)

(37-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.624

100

0.493
  pilE Neisseria gonorrhoeae MS11

40.244

100

0.446
  pilA2 Legionella pneumophila str. Paris

45.139

97.297

0.439
  pilA2 Legionella pneumophila strain ERS1305867

44.444

97.297

0.432
  pilA/pilA1 Eikenella corrodens VA1

38.415

100

0.426
  pilE Neisseria gonorrhoeae strain FA1090

36.905

100

0.419
  pilA Ralstonia pseudosolanacearum GMI1000

37.195

100

0.412
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.908

100

0.392
  comP Acinetobacter baylyi ADP1

37.671

98.649

0.372

Multiple sequence alignment