Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACNI65_RS01230 Genome accession   NZ_CP182288
Coordinates   274756..275994 (-) Length   412 a.a.
NCBI ID   WP_416760735.1    Uniprot ID   -
Organism   Roseateles sp. So40a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 269756..280994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNI65_RS01200 (ACNI65_01200) pgi 269806..271434 (+) 1629 WP_416760729.1 glucose-6-phosphate isomerase -
  ACNI65_RS01205 (ACNI65_01205) - 271509..272129 (+) 621 WP_416760730.1 HAD family hydrolase -
  ACNI65_RS01210 (ACNI65_01210) - 272130..272339 (-) 210 WP_416760731.1 DNA gyrase inhibitor YacG -
  ACNI65_RS01215 (ACNI65_01215) zapD 272352..273107 (-) 756 WP_416760732.1 cell division protein ZapD -
  ACNI65_RS01220 (ACNI65_01220) coaE 273183..273776 (-) 594 WP_416760733.1 dephospho-CoA kinase -
  ACNI65_RS01225 (ACNI65_01225) - 273796..274752 (-) 957 WP_416760734.1 prepilin peptidase -
  ACNI65_RS01230 (ACNI65_01230) pilC 274756..275994 (-) 1239 WP_416760735.1 type II secretion system F family protein Machinery gene
  ACNI65_RS01235 (ACNI65_01235) pilB 276120..277853 (-) 1734 WP_416760736.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNI65_RS01245 (ACNI65_01245) - 278261..279238 (-) 978 WP_416760737.1 polyprenyl synthetase family protein -
  ACNI65_RS01250 (ACNI65_01250) rplU 279492..279803 (+) 312 WP_416760738.1 50S ribosomal protein L21 -
  ACNI65_RS01255 (ACNI65_01255) rpmA 279840..280097 (+) 258 WP_416760739.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44888.81 Da        Isoelectric Point: 9.9788

>NTDB_id=1100984 ACNI65_RS01230 WP_416760735.1 274756..275994(-) (pilC) [Roseateles sp. So40a]
MATTTAGAKSAANKGIKEFVFEWEGKDRNGKIVRGEMRAGGEAVISASLRRQGILVTKVKKRRSSGGKSIKQKDIAVFTR
QLATMMRAGVPLLQAFDIVARGSPNPRLTKLLNDIRQDVETGTSLSTAFRKHPLYFDALYCNLVEAGEQGGILEALLDRL
AIYQEKTVALKGKIKSAMTYPIAVMVVAFVVTAVIMIFVIPAFKDVFKSFGADLPAPTLFVMAMSEIFVSYWYIIFGAIF
GGLYFFFQSWKRSEKMQKFMDRLLLKVPIFGQLIYKSAVARWTRTLATMFAAGVPLVEALDSVGGASGNAVFVEATEKIQ
RDVSTGASLTTSMQTTGIFPVMVLQMASIGEESGSLDHMLGKAADFYEEEVDDAVKALSSLMEPLIIVFLGGLIGGIVVS
MYLPIFKLGAVV

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=1100984 ACNI65_RS01230 WP_416760735.1 274756..275994(-) (pilC) [Roseateles sp. So40a]
ATGGCAACGACCACCGCGGGCGCGAAAAGCGCGGCCAACAAGGGCATCAAGGAATTCGTCTTCGAATGGGAAGGCAAGGA
CCGCAACGGCAAGATCGTTCGCGGGGAGATGCGCGCCGGCGGCGAGGCGGTGATCAGCGCGTCGCTGCGCCGCCAGGGCA
TCCTGGTCACCAAGGTCAAGAAGCGCCGCAGCTCCGGCGGCAAGAGCATCAAGCAGAAGGACATCGCCGTGTTCACGCGC
CAGCTGGCGACGATGATGCGCGCGGGCGTCCCGCTGCTGCAGGCCTTCGACATCGTGGCGCGAGGCAGTCCGAACCCCCG
CCTGACCAAGCTGCTGAACGACATCCGCCAGGACGTCGAGACCGGCACGTCGCTGTCGACGGCGTTCCGCAAGCATCCGC
TCTACTTCGACGCGCTGTACTGCAACCTGGTCGAGGCCGGCGAGCAGGGCGGTATCCTGGAAGCGCTGCTGGACCGGCTG
GCGATCTACCAGGAAAAGACCGTCGCGCTGAAGGGCAAGATCAAGTCGGCGATGACCTACCCGATCGCGGTGATGGTCGT
GGCCTTCGTCGTGACCGCGGTGATCATGATCTTCGTGATCCCGGCCTTCAAGGACGTGTTCAAGTCCTTCGGCGCCGACC
TGCCGGCCCCCACGCTGTTCGTGATGGCGATGTCGGAGATCTTCGTCTCCTACTGGTACATCATCTTCGGCGCCATCTTC
GGCGGGCTGTACTTCTTCTTTCAGAGCTGGAAACGCTCCGAGAAGATGCAGAAGTTCATGGACCGCCTGCTGCTGAAGGT
GCCCATCTTCGGCCAGCTGATCTACAAGTCCGCCGTCGCGCGCTGGACGCGCACGCTGGCGACGATGTTCGCCGCGGGCG
TGCCGCTGGTGGAAGCGCTGGACTCGGTCGGCGGTGCCTCGGGCAACGCCGTCTTCGTCGAGGCGACGGAGAAGATCCAG
CGTGACGTGTCGACTGGCGCGTCGCTGACGACCTCGATGCAGACGACCGGCATCTTCCCGGTGATGGTGCTGCAGATGGC
GTCGATCGGCGAGGAATCGGGCTCGCTGGACCACATGCTGGGCAAGGCCGCCGACTTCTACGAAGAAGAGGTGGATGACG
CCGTGAAGGCGCTGTCCAGCCTGATGGAACCGCTGATCATCGTGTTCCTGGGCGGATTGATCGGGGGCATCGTCGTGTCG
ATGTACCTGCCGATCTTCAAGCTCGGCGCGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)

Type II secretion system (T2SS), protein F (T2SSF)

(78-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.436

98.058

0.553
  pilG Neisseria meningitidis 44/76-A

53.133

96.845

0.515
  pilG Neisseria gonorrhoeae MS11

53.283

96.117

0.512
  pilC Legionella pneumophila strain ERS1305867

52.764

96.602

0.51
  pilC Acinetobacter baylyi ADP1

50.754

96.602

0.49
  pilC Acinetobacter baumannii D1279779

50.378

96.359

0.485
  pilC Vibrio cholerae strain A1552

42.172

96.117

0.405
  pilC Vibrio campbellii strain DS40M4

40.302

96.359

0.388
  pilC Thermus thermophilus HB27

39.599

96.845

0.383

Multiple sequence alignment