Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACNI65_RS01235 Genome accession   NZ_CP182288
Coordinates   276120..277853 (-) Length   577 a.a.
NCBI ID   WP_416760736.1    Uniprot ID   -
Organism   Roseateles sp. So40a     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 271120..282853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNI65_RS01205 (ACNI65_01205) - 271509..272129 (+) 621 WP_416760730.1 HAD family hydrolase -
  ACNI65_RS01210 (ACNI65_01210) - 272130..272339 (-) 210 WP_416760731.1 DNA gyrase inhibitor YacG -
  ACNI65_RS01215 (ACNI65_01215) zapD 272352..273107 (-) 756 WP_416760732.1 cell division protein ZapD -
  ACNI65_RS01220 (ACNI65_01220) coaE 273183..273776 (-) 594 WP_416760733.1 dephospho-CoA kinase -
  ACNI65_RS01225 (ACNI65_01225) - 273796..274752 (-) 957 WP_416760734.1 prepilin peptidase -
  ACNI65_RS01230 (ACNI65_01230) pilC 274756..275994 (-) 1239 WP_416760735.1 type II secretion system F family protein Machinery gene
  ACNI65_RS01235 (ACNI65_01235) pilB 276120..277853 (-) 1734 WP_416760736.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNI65_RS01245 (ACNI65_01245) - 278261..279238 (-) 978 WP_416760737.1 polyprenyl synthetase family protein -
  ACNI65_RS01250 (ACNI65_01250) rplU 279492..279803 (+) 312 WP_416760738.1 50S ribosomal protein L21 -
  ACNI65_RS01255 (ACNI65_01255) rpmA 279840..280097 (+) 258 WP_416760739.1 50S ribosomal protein L27 -
  ACNI65_RS01260 (ACNI65_01260) cgtA 280327..281523 (+) 1197 WP_416760740.1 Obg family GTPase CgtA -
  ACNI65_RS01265 (ACNI65_01265) proB 281591..282709 (+) 1119 WP_416763541.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63114.65 Da        Isoelectric Point: 5.8579

>NTDB_id=1100985 ACNI65_RS01235 WP_416760736.1 276120..277853(-) (pilB) [Roseateles sp. So40a]
MAIPVDTTLAEPPQSALSGVARVLVHAGKLKPQAAEDLVKLAREKKTSFVNAVMASGTVTPTDLAHTLSHALALPLMDLN
AVDAHKLPQNIIDAKLAAQYQVVVLARRGNRLFIGGADPTEQEVVERIKFATQLTPEWIIVEHDKLAKLLGITAASAAEA
LEAIAGEDFDFDIAEEESAPQEETQEVTDVEDAPVVRFLQKMLVDAINMRASDLHFEPFEYHYRVRFRVDGELREITQPP
IAIKDKLSSRIKVISRLDISERRVPQDGRMKLKFGSKAIDFRVSTLPTLFGEKIVIRILDPSSAKLGIEALGYEKIEKER
LLHCIHRPYGMILVTGPTGSGKTVSLYTCLNILNQPGTNISTVEDPAEINLPGINQVNVNDKAGLTFSAALKAFLRQDPD
IIMVGEIRDLETADIAIKAAQTGHMVMSTLHTNDAPTTLTRLLNMGVAPFNIASSVLLITAQRLARRLCENCKAPAEYPR
DALLRAGFVEADLDGSWKPYKAVGCSNCNNGYRGRVGLYQVMPISEAIQRIILSEGTAMDIAEQARADNVRDLRQSGLVK
VRAGVTTLEEVLAATNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1100985 ACNI65_RS01235 WP_416760736.1 276120..277853(-) (pilB) [Roseateles sp. So40a]
ATGGCGATACCCGTGGACACCACATTGGCCGAACCTCCTCAGTCCGCGCTCTCCGGCGTGGCGCGCGTGCTCGTGCACGC
GGGCAAGCTGAAGCCGCAGGCGGCCGAGGATCTGGTCAAGCTCGCGCGGGAGAAGAAGACCAGCTTCGTCAACGCGGTGA
TGGCCTCCGGCACGGTCACGCCCACGGACCTGGCGCACACGCTGTCGCACGCGCTCGCGCTGCCGCTGATGGACCTCAAC
GCGGTCGACGCGCACAAGCTCCCGCAGAACATCATCGACGCCAAGCTGGCCGCGCAGTACCAGGTCGTGGTGCTGGCGCG
CCGCGGCAACCGGCTGTTCATCGGCGGGGCGGACCCGACCGAGCAGGAAGTCGTCGAGCGCATCAAGTTCGCGACCCAGC
TGACGCCGGAATGGATCATCGTCGAGCACGACAAGCTCGCGAAGCTGCTGGGCATCACGGCGGCCTCGGCCGCGGAGGCG
CTCGAGGCGATCGCCGGCGAGGACTTCGACTTCGACATCGCCGAGGAAGAGTCGGCGCCACAGGAAGAGACGCAGGAGGT
CACCGACGTCGAGGACGCGCCGGTCGTGCGCTTCCTGCAGAAGATGCTGGTGGACGCGATCAACATGCGGGCGTCCGACC
TCCACTTCGAGCCCTTCGAGTACCACTACCGCGTCCGCTTCCGCGTCGACGGCGAGTTGCGCGAGATCACGCAGCCGCCC
ATCGCGATCAAGGACAAGCTGTCCTCGCGGATCAAGGTGATCTCGCGGCTGGACATCTCCGAGCGCCGCGTGCCGCAGGA
CGGCCGCATGAAGCTGAAGTTCGGCTCCAAGGCGATCGACTTCCGGGTCTCGACGCTGCCGACGCTCTTCGGCGAGAAGA
TCGTGATCCGGATCCTGGACCCGTCCTCGGCCAAGCTGGGGATCGAGGCGCTGGGTTACGAGAAGATCGAGAAGGAACGG
CTGCTGCACTGCATCCACCGCCCCTACGGGATGATCCTGGTCACGGGACCGACGGGCTCGGGCAAGACGGTGTCGCTGTA
CACCTGCCTGAACATCCTGAACCAGCCCGGCACGAACATCTCGACGGTCGAGGACCCCGCGGAAATCAACCTGCCCGGCA
TCAACCAGGTCAACGTCAACGACAAGGCGGGGCTGACCTTCTCCGCCGCGCTGAAGGCCTTCCTCCGTCAGGATCCGGAC
ATCATCATGGTCGGTGAGATCCGGGACCTGGAGACCGCCGACATCGCCATCAAGGCCGCGCAGACCGGCCACATGGTGAT
GTCGACGCTGCACACCAACGACGCGCCGACGACGCTGACGCGTCTGCTGAACATGGGCGTGGCGCCGTTCAACATCGCCT
CCAGCGTGCTGCTGATCACCGCGCAGCGTCTGGCGCGGCGCCTGTGCGAGAACTGCAAGGCGCCGGCGGAATATCCTCGC
GACGCACTGCTGCGCGCGGGTTTCGTGGAGGCGGACCTGGATGGCAGCTGGAAGCCGTACAAGGCCGTGGGCTGCTCGAA
TTGCAACAATGGTTATCGTGGACGTGTCGGTCTTTACCAAGTGATGCCCATCAGCGAGGCAATCCAGCGGATCATCCTGT
CCGAGGGAACGGCCATGGACATCGCCGAGCAGGCGCGCGCGGACAACGTGCGTGACCTGCGGCAGTCGGGGCTGGTGAAG
GTCCGGGCGGGCGTCACGACACTGGAAGAGGTGCTGGCGGCCACGAACGAGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-151)

Type II/IV secretion system protein (T2SSE)

(201-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.931

97.574

0.516
  pilF Neisseria gonorrhoeae MS11

52.465

98.44

0.516
  pilB Acinetobacter baylyi ADP1

51.309

99.307

0.51
  pilB Legionella pneumophila strain ERS1305867

50.178

97.4

0.489
  pilB Vibrio cholerae strain A1552

46.461

95.494

0.444
  pilB Vibrio parahaemolyticus RIMD 2210633

44.742

97.227

0.435
  pilB Vibrio campbellii strain DS40M4

44.662

97.4

0.435
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.478

98.96

0.371
  pilF Thermus thermophilus HB27

37.79

97.227

0.367

Multiple sequence alignment