Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACM1ZW_RS04360 Genome accession   NZ_CP182230
Coordinates   948626..949843 (+) Length   405 a.a.
NCBI ID   WP_017340818.1    Uniprot ID   A0A0K1QGJ9
Organism   Pseudomonas sp. NFX71     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 943626..954843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM1ZW_RS04340 (ACM1ZW_04340) - 945092..945388 (+) 297 WP_416740214.1 DUF2845 domain-containing protein -
  ACM1ZW_RS04345 (ACM1ZW_04345) - 945625..945981 (-) 357 WP_017340815.1 BON domain-containing protein -
  ACM1ZW_RS04350 (ACM1ZW_04350) - 946287..946697 (-) 411 WP_017340816.1 pilin -
  ACM1ZW_RS04355 (ACM1ZW_04355) pilB 946923..948623 (+) 1701 WP_017340817.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACM1ZW_RS04360 (ACM1ZW_04360) pilC 948626..949843 (+) 1218 WP_017340818.1 type II secretion system F family protein Machinery gene
  ACM1ZW_RS04365 (ACM1ZW_04365) pilD 949845..950717 (+) 873 WP_416740215.1 prepilin peptidase Machinery gene
  ACM1ZW_RS04370 (ACM1ZW_04370) coaE 950714..951337 (+) 624 WP_017340820.1 dephospho-CoA kinase -
  ACM1ZW_RS04375 (ACM1ZW_04375) yacG 951334..951534 (+) 201 WP_017340821.1 DNA gyrase inhibitor YacG -
  ACM1ZW_RS04380 (ACM1ZW_04380) - 951544..951759 (-) 216 WP_414878306.1 hypothetical protein -
  ACM1ZW_RS04385 (ACM1ZW_04385) - 951823..952512 (-) 690 WP_017340823.1 energy-coupling factor ABC transporter permease -
  ACM1ZW_RS04390 (ACM1ZW_04390) - 952700..952942 (+) 243 WP_414884100.1 DUF2790 domain-containing protein -
  ACM1ZW_RS04395 (ACM1ZW_04395) - 952961..953608 (-) 648 WP_416740216.1 methyl-accepting chemotaxis protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44141.98 Da        Isoelectric Point: 10.0024

>NTDB_id=1100902 ACM1ZW_RS04360 WP_017340818.1 948626..949843(+) (pilC) [Pseudomonas sp. NFX71]
MAVKAAKISVYAWEGTDKKGTKMTGELSGQNPALIKAQLRKQGINPGKVRKKSAAIFSLGQRIKAHDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPAMRKLVDDVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ESLKAKIKKAMTYPLAVVFVAIIVTGILLVKVVPQFESVFKGLGAQLPAFTVMVIGLSQFMQDWWWVMLGALIAAIFGAR
HAFRTSQGFRDRTDTWLLKLPLIGTLMYKSAVARYARTLSTTFAAGVPLVEALESVAGATGNIVFKRAVLRIKQDVSTGM
QLNFSMRTSGIFPNMAIQMTAIGEESGALDDMLDKVASFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVIAMYLPIFQ
LGNAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1100902 ACM1ZW_RS04360 WP_017340818.1 948626..949843(+) (pilC) [Pseudomonas sp. NFX71]
ATGGCAGTCAAAGCAGCGAAAATCAGCGTTTACGCCTGGGAAGGCACGGATAAAAAAGGCACGAAAATGACCGGCGAACT
CAGCGGTCAGAACCCGGCGCTGATCAAGGCGCAATTGCGCAAGCAAGGCATCAATCCCGGCAAGGTGCGCAAGAAGTCCG
CCGCGATTTTCAGCCTCGGCCAACGCATCAAGGCGCATGACATTGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAA
GCCGGGGTGCCGCTGTTGCAGTCGTTCGACATCATCGGCGAAGGCTTCGATAACCCGGCCATGCGCAAACTGGTGGACGA
CGTGAAACAGGAGGTCGCGGCGGGCAACAGTTTCGCCGCTGCCCTGCGTAAAAAACCGCAGTATTTCGATGAGCTCTACT
GCAACCTGGTGGATGCCGGCGAACAGGCCGGCGCGCTGGACACCCTGTTGGAGCGCGTCGCGACCTACAAGGAAAAGAGC
GAAAGCCTCAAGGCCAAGATCAAGAAAGCCATGACGTATCCCCTGGCCGTGGTGTTCGTCGCGATCATCGTCACCGGCAT
TCTGCTGGTCAAGGTGGTGCCGCAATTCGAGTCGGTGTTCAAAGGATTAGGTGCGCAATTGCCGGCGTTCACGGTGATGG
TCATCGGTCTGTCGCAGTTCATGCAGGATTGGTGGTGGGTGATGCTGGGGGCTTTGATTGCTGCGATATTCGGTGCGCGC
CACGCCTTCAGGACATCACAAGGCTTTCGCGACCGGACCGACACCTGGCTGTTGAAACTGCCGCTGATTGGCACGCTGAT
GTACAAGTCCGCCGTGGCCCGTTACGCCCGCACACTGTCGACCACCTTTGCCGCTGGCGTGCCGCTGGTCGAGGCGCTGG
AATCGGTGGCGGGCGCGACCGGCAACATCGTGTTCAAGCGTGCGGTGCTGCGCATCAAGCAGGATGTGTCGACAGGGATG
CAGCTGAATTTCTCCATGCGCACCTCCGGCATCTTTCCGAACATGGCGATCCAGATGACCGCCATTGGCGAAGAGTCCGG
CGCACTGGACGACATGCTCGACAAGGTCGCGAGTTTCTATGAGGCCGAAGTCGACAACATGGTCGATAACCTCACCAGTC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGGGTTATCGTCGGCGGCCTGGTGATTGCAATGTACCTGCCCATTTTCCAA
CTCGGCAATGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K1QGJ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

72.346

100

0.723
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Acinetobacter baumannii D1279779

58.578

100

0.59
  pilC Legionella pneumophila strain ERS1305867

53.283

97.778

0.521
  pilG Neisseria gonorrhoeae MS11

42.04

99.259

0.417
  pilC Vibrio cholerae strain A1552

42.857

96.79

0.415
  pilG Neisseria meningitidis 44/76-A

41.542

99.259

0.412
  pilC Vibrio campbellii strain DS40M4

40.547

99.259

0.402
  pilC Thermus thermophilus HB27

38.155

99.012

0.378

Multiple sequence alignment