Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   ACM67C_RS01980 Genome accession   NZ_CP181246
Coordinates   378952..380184 (-) Length   410 a.a.
NCBI ID   WP_066080057.1    Uniprot ID   A0A378UDG0
Organism   Bergeriella denitrificans strain DSM 17675     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 373952..385184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM67C_RS01950 (ACM67C_01950) ispH 374224..375192 (+) 969 WP_066079345.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ACM67C_RS01955 (ACM67C_01955) rarD 375264..376160 (-) 897 WP_066079342.1 EamA family transporter RarD -
  ACM67C_RS01960 (ACM67C_01960) - 376470..376928 (+) 459 WP_082790484.1 Lrp/AsnC family transcriptional regulator -
  ACM67C_RS01965 (ACM67C_01965) yacG 377189..377380 (-) 192 WP_066080048.1 DNA gyrase inhibitor YacG -
  ACM67C_RS01970 (ACM67C_01970) coaE 377442..378056 (-) 615 WP_066080051.1 dephospho-CoA kinase -
  ACM67C_RS01975 (ACM67C_01975) pilD 378087..378950 (-) 864 WP_066080054.1 prepilin peptidase Machinery gene
  ACM67C_RS01980 (ACM67C_01980) pilG 378952..380184 (-) 1233 WP_066080057.1 type II secretion system F family protein Machinery gene
  ACM67C_RS01985 (ACM67C_01985) pilF 380194..381873 (-) 1680 WP_066080060.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACM67C_RS01990 (ACM67C_01990) - 382449..382970 (-) 522 WP_066081396.1 pilin -
  ACM67C_RS01995 (ACM67C_01995) aroG 383795..384835 (-) 1041 WP_066080982.1 3-deoxy-7-phosphoheptulonate synthase AroG -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 44791.53 Da        Isoelectric Point: 9.9090

>NTDB_id=1099529 ACM67C_RS01980 WP_066080057.1 378952..380184(-) (pilG) [Bergeriella denitrificans strain DSM 17675]
MASTRPSSGRPAKGRRFAFEGKNTYTERLVRGEVVAENEEDAKRKLQRRGIRPTHISRVKSVRTRAITQEDITVFTRQLA
AMLKAGLPLLQAFEIIARGHANPAATQLLTGIRADVEQGSPLSASFAKHPKYFDRFYCNLVAAGETGGVLENLLDKLAAY
KEKTLAIKKKVKTMLMYPMAVMAVAAGLVFVMMRFVLPAFKEIYTNMGAQLPLMTQYAMTLSDLVVEYGWLMLVAAVAAV
VTLHRLHAKSYALQKRTDAMLLHLPLFGNIVRKATTARWLRTLSTLFAAGVPLVEALDSVAAASGNLLYEEATHDIRTSV
NQGMSLASSMQAADIFPNMVVQMAAVGEESGALDDMLNKAAEFYENEVDNSVSRLSALIEPVIMVIIGLIIGTLLIAMYL
PLLNLGGVVS

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=1099529 ACM67C_RS01980 WP_066080057.1 378952..380184(-) (pilG) [Bergeriella denitrificans strain DSM 17675]
ATGGCTTCCACACGCCCATCGTCCGGCCGCCCGGCCAAAGGCCGCCGCTTTGCCTTTGAAGGCAAAAACACCTACACCGA
GCGCCTTGTGCGCGGCGAAGTGGTGGCGGAAAACGAAGAAGACGCCAAACGCAAGCTGCAGCGGCGCGGCATCCGTCCAA
CGCACATCAGCCGCGTCAAATCCGTGCGTACCCGCGCCATTACCCAGGAAGACATTACCGTCTTCACCCGCCAGCTGGCC
GCCATGCTCAAAGCCGGTCTGCCGCTGCTGCAAGCCTTTGAAATCATCGCACGCGGCCATGCCAACCCCGCCGCCACGCA
GCTGCTGACCGGCATCCGCGCCGATGTAGAACAAGGCAGCCCGCTCTCGGCATCGTTTGCCAAGCATCCGAAATATTTCG
ACCGCTTCTACTGCAATCTGGTGGCGGCGGGCGAAACCGGCGGCGTATTGGAAAACCTGCTCGACAAGCTGGCCGCGTAT
AAAGAAAAAACGCTGGCCATCAAGAAAAAAGTCAAAACCATGCTGATGTATCCGATGGCGGTTATGGCCGTCGCCGCCGG
CCTGGTCTTTGTGATGATGCGCTTTGTGCTGCCCGCATTTAAGGAAATCTACACCAATATGGGCGCGCAGCTGCCGCTGA
TGACGCAATATGCCATGACGCTCTCGGATTTGGTGGTGGAATACGGCTGGCTGATGCTGGTAGCCGCCGTGGCCGCCGTG
GTGACGCTGCACCGGCTGCACGCGAAATCGTATGCCCTGCAAAAGCGCACCGATGCCATGCTGCTGCATCTGCCCCTTTT
CGGCAACATCGTGCGTAAAGCCACCACCGCCCGCTGGCTGCGTACCCTTTCCACCCTGTTTGCCGCCGGCGTGCCGCTGG
TGGAAGCGCTGGATTCCGTGGCCGCCGCTTCGGGCAACCTGCTCTACGAAGAAGCCACCCACGATATCCGCACCAGCGTC
AATCAGGGGATGTCGCTGGCTTCCAGTATGCAGGCCGCCGATATTTTTCCCAATATGGTTGTGCAGATGGCGGCGGTGGG
CGAAGAATCCGGCGCACTCGACGATATGCTCAACAAAGCGGCGGAATTTTATGAAAACGAAGTGGACAATTCGGTTTCCC
GTCTGTCTGCCCTGATTGAACCCGTTATCATGGTCATCATCGGTCTGATTATCGGCACGCTGCTGATTGCCATGTATCTG
CCGCTGCTCAACCTGGGCGGCGTGGTGTCTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378UDG0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

66.08

97.073

0.641
  pilG Neisseria meningitidis 44/76-A

65.327

97.073

0.634
  pilC Legionella pneumophila strain ERS1305867

44.361

97.317

0.432
  pilC Pseudomonas stutzeri DSM 10701

43.641

97.805

0.427
  pilC Acinetobacter baumannii D1279779

42.714

97.073

0.415
  pilC Acinetobacter baylyi ADP1

41.457

97.073

0.402
  pilC Thermus thermophilus HB27

38.677

95.854

0.371

Multiple sequence alignment