Detailed information NTG1099528

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACM67C_RS01975
• Genome accession: NZ_CP181246
• Coordinates: 378087..378950 (-)
• Length: 287 a.a.
• NCBI ID: WP_066080054.1
• Uniprot ID: A0A378UD67
• Organism: Bergeriella denitrificans strain DSM 17675
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 373087..383950

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACM67C_RS01945 (ACM67C_01945)	lspA	373674..374174 (+)	501	WP_066079349.1	signal peptidase II	-
ACM67C_RS01950 (ACM67C_01950)	ispH	374224..375192 (+)	969	WP_066079345.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
ACM67C_RS01955 (ACM67C_01955)	rarD	375264..376160 (-)	897	WP_066079342.1	EamA family transporter RarD	-
ACM67C_RS01960 (ACM67C_01960)	-	376470..376928 (+)	459	WP_082790484.1	Lrp/AsnC family transcriptional regulator	-
ACM67C_RS01965 (ACM67C_01965)	yacG	377189..377380 (-)	192	WP_066080048.1	DNA gyrase inhibitor YacG	-
ACM67C_RS01970 (ACM67C_01970)	coaE	377442..378056 (-)	615	WP_066080051.1	dephospho-CoA kinase	-
ACM67C_RS01975 (ACM67C_01975)	pilD	378087..378950 (-)	864	WP_066080054.1	prepilin peptidase	Machinery gene
ACM67C_RS01980 (ACM67C_01980)	pilG	378952..380184 (-)	1233	WP_066080057.1	type II secretion system F family protein	Machinery gene
ACM67C_RS01985 (ACM67C_01985)	pilF	380194..381873 (-)	1680	WP_066080060.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACM67C_RS01990 (ACM67C_01990)	-	382449..382970 (-)	522	WP_066081396.1	pilin	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 30764.80 Da
• Isoelectric Point: 8.3713

>NTDB_id=1099528 ACM67C_RS01975 WP_066080054.1 378087..378950(-) (pilD) [Bergeriella denitrificans strain DSM 17675]
MQSLLESSAPLLLFFSVILGLMIGSFLNVVIYRLPVMMERDWTLFAKEHLGIRPSEKDAAPFNLAVPPSHCPHCGSPVRA
LQNIPLLSYLLLRGRCAACRAPIGKRYPLVELLTALAFAAVAWQYGASWLTLGGWVLSAFLIALAFIDADTRYLPDTLTL
PLIWLGLLFNLGGGLTDLESAVIGAVCGYLSLWLLCAAYKLLTGQTGMGGGDFKLLAALGAWLGAAVLPLLVFAASLAGI
LAALMLKIGKNQAFAFGPCLALAGWILMLAYAPAQQLVRWWLSASGF

Nucleotide

• Length: 864 bp

>NTDB_id=1099528 ACM67C_RS01975 WP_066080054.1 378087..378950(-) (pilD) [Bergeriella denitrificans strain DSM 17675]
ATGCAGAGCCTGCTTGAATCTTCCGCACCGCTGCTGCTGTTTTTTTCGGTCATACTCGGCCTGATGATCGGCAGCTTTCT
CAACGTGGTCATCTACCGCCTGCCCGTGATGATGGAGCGGGATTGGACCCTGTTTGCCAAAGAACATTTGGGCATCAGGC
CGTCTGAAAAAGACGCCGCACCCTTCAATCTGGCCGTGCCGCCTTCGCATTGCCCGCATTGCGGCAGCCCCGTGCGCGCC
CTGCAGAATATCCCGCTGCTCAGCTACCTGCTGCTGCGCGGCCGCTGCGCCGCCTGCCGCGCCCCCATCGGCAAACGCTA
TCCGCTGGTGGAGCTGCTGACCGCGCTGGCCTTTGCCGCCGTTGCCTGGCAATACGGCGCAAGCTGGCTGACTTTAGGCG
GCTGGGTGCTCAGCGCCTTTCTGATTGCGCTGGCCTTTATCGATGCCGATACCCGCTACCTGCCCGATACCCTGACCCTG
CCGCTGATTTGGCTGGGGCTGCTGTTCAACCTCGGCGGCGGGCTGACCGATCTCGAATCCGCCGTGATCGGCGCGGTGTG
CGGCTATCTGAGCCTGTGGCTGCTGTGTGCCGCCTACAAACTGCTCACCGGCCAAACCGGCATGGGCGGCGGCGACTTCA
AGCTGCTGGCCGCGCTCGGCGCCTGGCTGGGCGCGGCGGTGCTGCCACTGCTCGTGTTTGCCGCATCGCTGGCCGGTATT
CTCGCCGCGCTGATGCTGAAAATCGGCAAAAACCAAGCCTTCGCCTTCGGCCCCTGCCTCGCGCTGGCCGGCTGGATTTT
GATGCTGGCCTACGCACCCGCGCAGCAGCTGGTACGCTGGTGGCTCTCCGCATCGGGATTTTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-125)

• Type IV leader peptidase family (Peptidase_A24) (135-243)

3D structure

• AlphaFold DB: A0A378UD67

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	60.498	97.909	0.592
pilD	Vibrio cholerae strain A1552	48.014	96.516	0.463
pilD	Vibrio campbellii strain DS40M4	45.52	97.213	0.443
pilD	Acinetobacter nosocomialis M2	49.213	88.502	0.436
pilD	Acinetobacter baumannii D1279779	48.819	88.502	0.432