Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACM67C_RS01985 Genome accession   NZ_CP181246
Coordinates   380194..381873 (-) Length   559 a.a.
NCBI ID   WP_066080060.1    Uniprot ID   -
Organism   Bergeriella denitrificans strain DSM 17675     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 375194..386873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM67C_RS01955 (ACM67C_01955) rarD 375264..376160 (-) 897 WP_066079342.1 EamA family transporter RarD -
  ACM67C_RS01960 (ACM67C_01960) - 376470..376928 (+) 459 WP_082790484.1 Lrp/AsnC family transcriptional regulator -
  ACM67C_RS01965 (ACM67C_01965) yacG 377189..377380 (-) 192 WP_066080048.1 DNA gyrase inhibitor YacG -
  ACM67C_RS01970 (ACM67C_01970) coaE 377442..378056 (-) 615 WP_066080051.1 dephospho-CoA kinase -
  ACM67C_RS01975 (ACM67C_01975) pilD 378087..378950 (-) 864 WP_066080054.1 prepilin peptidase Machinery gene
  ACM67C_RS01980 (ACM67C_01980) pilG 378952..380184 (-) 1233 WP_066080057.1 type II secretion system F family protein Machinery gene
  ACM67C_RS01985 (ACM67C_01985) pilF 380194..381873 (-) 1680 WP_066080060.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACM67C_RS01990 (ACM67C_01990) - 382449..382970 (-) 522 WP_066081396.1 pilin -
  ACM67C_RS01995 (ACM67C_01995) aroG 383795..384835 (-) 1041 WP_066080982.1 3-deoxy-7-phosphoheptulonate synthase AroG -
  ACM67C_RS02000 (ACM67C_02000) - 384942..385661 (-) 720 WP_066080985.1 hypothetical protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 61685.00 Da        Isoelectric Point: 6.4383

>NTDB_id=1099530 ACM67C_RS01985 WP_066080060.1 380194..381873(-) (pilF) [Bergeriella denitrificans strain DSM 17675]
MSVGLLRILVQNHILTPDQAGRYMDTAHAGRALMPRLFADGVTTPRALAGLLAQTFNYPLLDLHHFPRSGVLADVLTEEQ
MIQNRCVPLFRRGRTVYLGVSDPTRIQDFQKMAFASGEDMDLVVVRDDQLEALLEWLGQRSTAILSKIRREHEAAQGTQI
LYIDNEEAEDGPIPRFIHKTLSDAIQAGASDIHFEFYEHIARVRFRVDGQLREVVQPPVAVRGQLASRIKVMAHLDIAEK
RVPQDGRTQIAFQKNGRPVDFRVSTLPTLFGEKVVMRILNSDIGGLDIDTLGLEPFQKTMLLEAIRRPYGMVLVTGPTGS
GKTVSLYTCLNILNTESVNIATAEDPAEINLPGVNQVNVNDKQGLTFASALRAFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRLLNLGVAPFNIASSITLIMAQRLLRRLCPDCKKAVPRPPEAALKQAGFTDEDLAGSWTL
YRPAGCESCRGSGFKGRIGVYEVMPVSEAMQRVIMDNGTEVDITALAYQEGMVDLRRAGLLKVMQGLTPLEEVTARTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1099530 ACM67C_RS01985 WP_066080060.1 380194..381873(-) (pilF) [Bergeriella denitrificans strain DSM 17675]
ATGAGCGTCGGATTATTGCGTATCCTGGTACAAAACCACATTCTGACACCCGATCAGGCAGGCCGCTATATGGACACCGC
CCACGCCGGGCGCGCGCTGATGCCCCGTCTGTTTGCCGACGGCGTCACCACCCCGCGCGCATTGGCCGGGCTGCTGGCGC
AAACCTTCAATTATCCCCTGCTGGATTTGCACCATTTTCCGCGCAGCGGCGTCTTGGCCGATGTACTGACCGAAGAGCAG
ATGATTCAAAACCGCTGCGTGCCGCTGTTCCGGCGCGGGCGCACGGTATATCTGGGCGTATCCGATCCCACGCGGATTCA
GGATTTCCAAAAAATGGCTTTTGCCTCCGGCGAAGACATGGACTTGGTTGTGGTGCGCGACGACCAGCTCGAAGCGCTGC
TGGAGTGGCTGGGGCAGCGTTCCACCGCCATTCTGAGCAAAATCCGCCGCGAGCACGAAGCCGCGCAGGGCACGCAAATT
CTCTATATCGACAACGAAGAAGCCGAAGACGGCCCGATTCCGCGCTTTATCCACAAAACCCTTTCCGATGCCATTCAGGC
CGGCGCGTCCGACATCCATTTTGAATTTTACGAACACATCGCACGGGTGCGCTTCCGCGTGGACGGCCAGCTGCGCGAAG
TGGTGCAGCCGCCCGTGGCCGTGCGCGGCCAGCTTGCCTCGCGCATCAAAGTCATGGCGCATCTGGATATTGCTGAAAAG
CGCGTGCCGCAAGACGGGCGCACCCAAATCGCTTTCCAGAAAAACGGCCGCCCCGTGGATTTCCGCGTCAGCACCCTGCC
CACCCTGTTTGGCGAAAAAGTCGTGATGCGGATTTTAAATTCCGATATCGGCGGTTTGGACATCGACACCTTGGGTTTGG
AGCCGTTTCAAAAAACCATGCTGCTCGAAGCCATCCGACGCCCTTACGGCATGGTCTTGGTCACCGGCCCCACCGGCTCG
GGCAAAACCGTGTCGCTCTACACCTGCCTGAATATTCTGAATACCGAAAGCGTCAACATCGCCACGGCGGAAGACCCGGC
GGAAATCAACCTGCCCGGCGTCAACCAAGTCAATGTCAACGACAAACAGGGCCTCACATTCGCATCCGCGCTGCGCGCCT
TTCTGCGCCAAGACCCCGACATCATCATGGTGGGCGAAATCCGCGATTTAGAAACCGCCGACATCGCCATCAAAGCCGCG
CAAACCGGGCATATGGTGTTCTCCACCCTGCACACCAACAACGCGCCCGCTACCCTTTCGCGCCTGCTCAATCTGGGCGT
TGCCCCCTTCAACATCGCCAGCTCCATCACGCTGATTATGGCGCAACGCCTACTGCGCCGCCTCTGCCCCGACTGCAAAA
AAGCCGTGCCCCGCCCGCCCGAAGCCGCGTTGAAGCAGGCCGGTTTTACCGATGAAGACCTGGCCGGCAGCTGGACGCTC
TACCGCCCCGCAGGCTGCGAATCCTGCCGCGGCAGTGGGTTCAAAGGACGCATCGGCGTGTATGAAGTCATGCCGGTCAG
CGAAGCGATGCAGCGCGTCATCATGGACAACGGCACGGAAGTGGACATCACCGCGCTGGCCTATCAGGAAGGCATGGTCG
ACTTGCGCCGCGCCGGCCTGCTCAAGGTGATGCAGGGGCTGACCCCTTTGGAAGAAGTAACCGCCCGCACCAACGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-139)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

73.703

100

0.737
  pilB Acinetobacter baylyi ADP1

48.932

100

0.492
  pilB Acinetobacter baumannii D1279779

48.313

100

0.487
  pilB Legionella pneumophila strain ERS1305867

46.466

100

0.47
  pilB Vibrio parahaemolyticus RIMD 2210633

44.149

100

0.445
  pilB Vibrio cholerae strain A1552

44.627

98.211

0.438
  pilB Vibrio campbellii strain DS40M4

42.908

100

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.532

97.496

0.376
  pilF Thermus thermophilus HB27

39.394

94.454

0.372

Multiple sequence alignment