Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACM0CZ_RS03555 Genome accession   NZ_CP180559
Coordinates   740579..741688 (-) Length   369 a.a.
NCBI ID   WP_411637469.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain BJ014     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 735579..746688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM0CZ_RS03535 brnQ 736036..737325 (-) 1290 WP_187268869.1 branched-chain amino acid transport system II carrier protein -
  ACM0CZ_RS03540 - 737535..738245 (-) 711 WP_411637467.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  ACM0CZ_RS03545 srmB 738374..739597 (+) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  ACM0CZ_RS03550 yaaA 739774..740547 (-) 774 WP_411637468.1 peroxide stress protein YaaA -
  ACM0CZ_RS03555 pilU 740579..741688 (-) 1110 WP_411637469.1 type IVa pilus ATPase TapU Machinery gene
  ACM0CZ_RS03560 pilT 741717..742751 (-) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  ACM0CZ_RS03565 - 742791..743492 (+) 702 WP_411637470.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACM0CZ_RS03570 proC 743551..744375 (+) 825 WP_411637471.1 pyrroline-5-carboxylate reductase -
  ACM0CZ_RS03575 - 744416..744967 (+) 552 WP_017411214.1 YggT family protein -
  ACM0CZ_RS03580 yggU 744967..745266 (+) 300 WP_011707385.1 DUF167 family protein YggU -
  ACM0CZ_RS03585 - 745285..745704 (+) 420 WP_011707384.1 DUF4426 domain-containing protein -
  ACM0CZ_RS03590 - 745761..746072 (-) 312 WP_207137359.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40981.13 Da        Isoelectric Point: 6.3880

>NTDB_id=1096613 ACM0CZ_RS03555 WP_411637469.1 740579..741688(-) (pilU) [Aeromonas hydrophila strain BJ014]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLTEAPLDKAGALALVKETLSPEHFERYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHMDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1096613 ACM0CZ_RS03555 WP_411637469.1 740579..741688(-) (pilU) [Aeromonas hydrophila strain BJ014]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCGCTGACAGAAGCCCCCCTCGACAAGGCAGGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCCTGAGCACTTCGAGCGCTATCTGCACACCAAGGAGGCCAACTACGCCATTCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGTGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGTATCGAGACCCGCATTCCCACCTTTGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAGATCGCCCTGGCCAAGCGCGGGCTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAATCCACCACCCAGGCGGCCATGATCGGTTATCGCAACCAGCATATGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACTCAGCGCGAGGTGGGCATAGACACAGCGTCGTTCGACGT
GGCGCTGAAGAGTTCGCTGCGCCAGGCCCCTGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACAGGTCATCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGACGGGCGCCGTCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCCCTCATCACCGATATCA
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTAATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363

Multiple sequence alignment