Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACMXYQ_RS13435 Genome accession   NZ_CP180544
Coordinates   2959178..2960398 (+) Length   406 a.a.
NCBI ID   WP_415895018.1    Uniprot ID   -
Organism   Neptuniibacter sp. PT34_22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2954178..2965398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYQ_RS13410 (ACMXYQ_13410) ampD 2954562..2955107 (-) 546 WP_415895013.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACMXYQ_RS13415 (ACMXYQ_13415) nadC 2955209..2956054 (+) 846 WP_415895014.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACMXYQ_RS13420 (ACMXYQ_13420) - 2956139..2956480 (-) 342 WP_415895015.1 hypothetical protein -
  ACMXYQ_RS13425 (ACMXYQ_13425) - 2956689..2957204 (-) 516 WP_415895016.1 pilin -
  ACMXYQ_RS13430 (ACMXYQ_13430) pilB 2957446..2959161 (+) 1716 WP_415895017.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMXYQ_RS13435 (ACMXYQ_13435) pilC 2959178..2960398 (+) 1221 WP_415895018.1 type II secretion system F family protein Machinery gene
  ACMXYQ_RS13440 (ACMXYQ_13440) pilD 2960411..2961259 (+) 849 WP_415893588.1 prepilin peptidase Machinery gene
  ACMXYQ_RS13445 (ACMXYQ_13445) coaE 2961263..2961865 (+) 603 WP_415895019.1 dephospho-CoA kinase -
  ACMXYQ_RS13450 (ACMXYQ_13450) yacG 2961865..2962092 (+) 228 WP_415893586.1 DNA gyrase inhibitor YacG -
  ACMXYQ_RS13455 (ACMXYQ_13455) - 2962070..2963029 (-) 960 WP_415895020.1 Nudix family hydrolase -
  ACMXYQ_RS13460 (ACMXYQ_13460) argJ 2963029..2964249 (-) 1221 WP_415895021.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 43999.22 Da        Isoelectric Point: 9.3460

>NTDB_id=1096355 ACMXYQ_RS13435 WP_415895018.1 2959178..2960398(+) (pilC) [Neptuniibacter sp. PT34_22]
MAKDAKLSTFTWQGKDKSGNKSKGKIDANNAASAKALLRKQGILTSRIAKERSAGMFSAKNKSVKPVDIAFFTRQMATMM
RSGVPLLQGLEITAGGIEKQKLKDIVYEIKNDVNSGTDFSTALTKHPQHFDDLYCNLVNAGEQSGALDAMLDRIATYKEK
LESLKAKIKKALTYPTAVVVVGIIVSAILLVKVVPQFEDVFNSFGAELPAFTQWVINLSEIAQEWWWVALIGVVLFGFAF
QKAREKSAKFRDGVDKTVLKIPVIGEILHNASIARFARTLSTTFAAGVPLVNALDSAAGASGNALYRDAILQIKNGVSTG
QSLQNAVTMTGIFPNMTIQMISIGEEAGSLEMMLEKIADFYEEQVDNAVDNLSSLLEPLIMVVLGVLVGGLVVAMYLPIF
QLGQVV

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1096355 ACMXYQ_RS13435 WP_415895018.1 2959178..2960398(+) (pilC) [Neptuniibacter sp. PT34_22]
ATGGCTAAAGATGCCAAGCTAAGTACTTTCACCTGGCAAGGGAAAGATAAAAGCGGTAATAAGAGCAAAGGGAAGATTGA
TGCTAACAACGCGGCATCAGCAAAGGCCCTTCTTCGAAAGCAAGGAATCTTAACATCAAGAATTGCTAAAGAGCGCTCTG
CAGGCATGTTCAGCGCAAAAAACAAATCCGTTAAGCCTGTTGATATTGCTTTTTTTACCCGTCAAATGGCTACCATGATG
CGTTCTGGTGTACCTCTACTACAAGGCCTAGAGATAACCGCTGGTGGTATTGAAAAACAAAAGCTGAAAGACATCGTCTA
TGAAATCAAGAATGATGTTAACAGCGGCACAGATTTTTCTACAGCTTTGACCAAACATCCACAACACTTCGATGATCTGT
ATTGCAATTTAGTTAATGCTGGTGAGCAATCTGGTGCCCTAGATGCAATGCTGGACCGCATCGCGACTTATAAAGAAAAG
CTAGAATCATTAAAAGCTAAGATCAAAAAAGCACTCACCTACCCTACCGCCGTAGTTGTAGTCGGCATTATTGTTTCGGC
AATTCTTCTAGTTAAAGTAGTGCCACAATTTGAAGATGTATTTAATAGCTTCGGAGCAGAATTACCGGCATTTACGCAAT
GGGTTATAAATCTATCCGAAATTGCTCAAGAATGGTGGTGGGTTGCTTTAATTGGCGTTGTCTTATTTGGTTTCGCGTTT
CAAAAAGCCCGAGAAAAATCAGCTAAGTTTAGAGATGGAGTCGACAAAACTGTACTTAAAATCCCAGTTATTGGTGAAAT
ACTGCACAATGCATCTATAGCAAGGTTTGCGCGAACCTTATCAACCACGTTTGCAGCAGGTGTACCTTTAGTTAATGCTT
TAGATTCAGCTGCTGGCGCTTCAGGCAATGCGCTTTATAGGGACGCCATCTTACAGATCAAAAACGGTGTTTCTACAGGT
CAGTCTCTACAAAACGCCGTCACAATGACTGGCATTTTTCCAAACATGACAATCCAGATGATTTCTATAGGTGAAGAAGC
CGGCTCCCTTGAAATGATGCTAGAGAAAATCGCCGATTTTTATGAAGAACAAGTAGATAACGCTGTAGATAACCTTTCAA
GCTTACTAGAGCCTTTAATTATGGTTGTATTGGGCGTGCTAGTTGGTGGGCTTGTTGTGGCAATGTACCTTCCAATCTTC
CAGCTTGGTCAAGTTGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.985

100

0.581
  pilC Legionella pneumophila strain ERS1305867

55.831

99.261

0.554
  pilC Acinetobacter baumannii D1279779

54.5

98.522

0.537
  pilC Acinetobacter baylyi ADP1

53.846

99.261

0.534
  pilC Vibrio cholerae strain A1552

42.537

99.015

0.421
  pilG Neisseria meningitidis 44/76-A

42.04

99.015

0.416
  pilG Neisseria gonorrhoeae MS11

42.04

99.015

0.416
  pilC Vibrio campbellii strain DS40M4

41.895

98.768

0.414

Multiple sequence alignment