Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACMXYQ_RS13440 Genome accession   NZ_CP180544
Coordinates   2960411..2961259 (+) Length   282 a.a.
NCBI ID   WP_415893588.1    Uniprot ID   -
Organism   Neptuniibacter sp. PT34_22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2955411..2966259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYQ_RS13420 (ACMXYQ_13420) - 2956139..2956480 (-) 342 WP_415895015.1 hypothetical protein -
  ACMXYQ_RS13425 (ACMXYQ_13425) - 2956689..2957204 (-) 516 WP_415895016.1 pilin -
  ACMXYQ_RS13430 (ACMXYQ_13430) pilB 2957446..2959161 (+) 1716 WP_415895017.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMXYQ_RS13435 (ACMXYQ_13435) pilC 2959178..2960398 (+) 1221 WP_415895018.1 type II secretion system F family protein Machinery gene
  ACMXYQ_RS13440 (ACMXYQ_13440) pilD 2960411..2961259 (+) 849 WP_415893588.1 prepilin peptidase Machinery gene
  ACMXYQ_RS13445 (ACMXYQ_13445) coaE 2961263..2961865 (+) 603 WP_415895019.1 dephospho-CoA kinase -
  ACMXYQ_RS13450 (ACMXYQ_13450) yacG 2961865..2962092 (+) 228 WP_415893586.1 DNA gyrase inhibitor YacG -
  ACMXYQ_RS13455 (ACMXYQ_13455) - 2962070..2963029 (-) 960 WP_415895020.1 Nudix family hydrolase -
  ACMXYQ_RS13460 (ACMXYQ_13460) argJ 2963029..2964249 (-) 1221 WP_415895021.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31755.71 Da        Isoelectric Point: 6.7232

>NTDB_id=1096356 ACMXYQ_RS13440 WP_415893588.1 2960411..2961259(+) (pilD) [Neptuniibacter sp. PT34_22]
MNTIIFDPAWAIACAFLFSLCVGSFLNVVIHRLPIMMQREWEREISADEEVNQEPYNLSVPASTCPKCQHKIRWYENIPV
ISWIFLRAKCSSCGLHIPFRYPFVELFTAICSAYAVYVFGFNLIGLSVVLLTWVLVCLIFIDIDHQLLPDRLTLPLLWLG
LFVNSFGLFSSLEHAVYGAMAGYLSLWSIYWAFKLLTGKEGMGYGDFKLLAALGAWAGVYQLPLIILLSSLVGAIIGIGM
MLFKNHSSQNPIPFGPYLAIAGWIALIWGDTLTTIYLQLIRI

Nucleotide


Download         Length: 849 bp        

>NTDB_id=1096356 ACMXYQ_RS13440 WP_415893588.1 2960411..2961259(+) (pilD) [Neptuniibacter sp. PT34_22]
ATGAATACAATTATTTTTGATCCCGCGTGGGCTATTGCCTGCGCGTTTTTATTTTCCCTTTGTGTCGGCAGCTTCTTAAA
CGTAGTCATACATCGTTTACCCATTATGATGCAAAGAGAATGGGAGAGAGAAATATCTGCTGATGAAGAGGTAAATCAAG
AGCCTTACAACCTTTCAGTACCTGCATCCACTTGCCCTAAGTGCCAACACAAAATCCGCTGGTATGAAAACATTCCTGTC
ATCAGTTGGATTTTCCTTCGGGCCAAATGTTCATCTTGTGGACTGCATATCCCTTTTCGATACCCTTTTGTAGAATTATT
TACAGCTATTTGCTCCGCTTATGCCGTTTACGTTTTTGGCTTTAACCTGATTGGATTAAGCGTAGTATTACTTACATGGG
TACTGGTATGCCTGATTTTTATAGATATTGATCATCAACTACTTCCCGACCGTTTAACTCTACCTTTGTTATGGCTGGGT
TTATTCGTCAACAGTTTTGGCCTTTTCAGTTCACTAGAACACGCTGTCTATGGTGCAATGGCAGGTTACCTAAGCTTATG
GTCAATCTACTGGGCATTTAAACTGCTAACGGGCAAAGAAGGTATGGGATACGGTGATTTCAAACTACTCGCAGCCCTTG
GAGCCTGGGCTGGAGTCTATCAATTGCCGCTTATTATCCTCTTAAGCTCCCTCGTCGGTGCAATCATTGGGATAGGCATG
ATGCTATTTAAAAACCATTCATCGCAAAACCCAATTCCATTTGGCCCTTACCTCGCTATTGCGGGCTGGATAGCGCTGAT
ATGGGGCGATACTCTTACGACAATCTACCTGCAACTTATAAGAATCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-118)

Type IV leader peptidase family (Peptidase_A24)

(130-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.39

95.39

0.528
  pilD Acinetobacter baumannii D1279779

55.263

94.326

0.521
  pilD Vibrio campbellii strain DS40M4

51.049

100

0.518
  pilD Acinetobacter nosocomialis M2

54.887

94.326

0.518
  pilD Neisseria gonorrhoeae MS11

46.097

95.39

0.44

Multiple sequence alignment