Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMXYQ_RS13430 Genome accession   NZ_CP180544
Coordinates   2957446..2959161 (+) Length   571 a.a.
NCBI ID   WP_415895017.1    Uniprot ID   -
Organism   Neptuniibacter sp. PT34_22     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2952446..2964161
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYQ_RS13405 (ACMXYQ_13405) - 2953854..2954561 (+) 708 WP_415895012.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACMXYQ_RS13410 (ACMXYQ_13410) ampD 2954562..2955107 (-) 546 WP_415895013.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACMXYQ_RS13415 (ACMXYQ_13415) nadC 2955209..2956054 (+) 846 WP_415895014.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACMXYQ_RS13420 (ACMXYQ_13420) - 2956139..2956480 (-) 342 WP_415895015.1 hypothetical protein -
  ACMXYQ_RS13425 (ACMXYQ_13425) - 2956689..2957204 (-) 516 WP_415895016.1 pilin -
  ACMXYQ_RS13430 (ACMXYQ_13430) pilB 2957446..2959161 (+) 1716 WP_415895017.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMXYQ_RS13435 (ACMXYQ_13435) pilC 2959178..2960398 (+) 1221 WP_415895018.1 type II secretion system F family protein Machinery gene
  ACMXYQ_RS13440 (ACMXYQ_13440) pilD 2960411..2961259 (+) 849 WP_415893588.1 prepilin peptidase Machinery gene
  ACMXYQ_RS13445 (ACMXYQ_13445) coaE 2961263..2961865 (+) 603 WP_415895019.1 dephospho-CoA kinase -
  ACMXYQ_RS13450 (ACMXYQ_13450) yacG 2961865..2962092 (+) 228 WP_415893586.1 DNA gyrase inhibitor YacG -
  ACMXYQ_RS13455 (ACMXYQ_13455) - 2962070..2963029 (-) 960 WP_415895020.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63128.98 Da        Isoelectric Point: 4.7750

>NTDB_id=1096354 ACMXYQ_RS13430 WP_415895017.1 2957446..2959161(+) (pilB) [Neptuniibacter sp. PT34_22]
MQPSQPLSGLAKRLVNEGVCSAEVAADASLQSREKQQTFISYVIEQRLVSAYRAAAAASEEFGMPLLDLNALDPASLPQG
LINDSLITKHHALPLQKRDNRLFVAISDPTNIQALDEFKFQSGITTEAIIVEEDKLTRAIEVFLEGQDSALDELEDSDLD
NLEISDGATEEQEEQESTEDATKDAPIVRFINKILLDSIKNGASDIHFEPYEKSYRIRSRIDGILQEVAKPPSNLAARLS
ARLKVMSQMDISEKRVPQDGRIKMKISKKRAIDFRVNTLPTLWGEKIVLRILDPSSAKMGVEALGFAEFQKELYLGTLHR
PQGMVLVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVHVNAKVGLNFAEALRAFLRQDPDVVMVGEI
RDLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPAFNIATSVSLIIAQRLGRRLCESCKKPITLPEVTLKEEG
FTDEEINDLTLFEANQDGCSKCNRGYKGRVGIYEMLPMTPEIAEVIMENGNSLDILRVAETQNFQSLRRSGLKKVAEGIT
SLEEMNRVIKT

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1096354 ACMXYQ_RS13430 WP_415895017.1 2957446..2959161(+) (pilB) [Neptuniibacter sp. PT34_22]
TTGCAGCCAAGCCAACCTTTAAGCGGACTTGCCAAGCGATTGGTCAATGAAGGCGTATGCTCTGCGGAAGTTGCAGCGGA
TGCCTCTCTACAGTCAAGAGAGAAGCAGCAAACATTTATATCTTATGTGATTGAACAACGTTTAGTTAGCGCCTACAGAG
CAGCAGCTGCCGCATCTGAAGAGTTTGGCATGCCGCTATTAGATTTGAACGCTTTAGACCCAGCATCCCTTCCTCAAGGA
CTAATTAACGATTCCCTAATAACTAAACATCATGCACTCCCTCTTCAAAAAAGAGATAACCGTTTATTTGTAGCCATTTC
GGACCCTACAAATATTCAGGCATTAGATGAGTTTAAATTCCAAAGCGGCATTACCACTGAAGCCATTATTGTTGAAGAAG
ATAAGCTCACACGGGCAATTGAAGTTTTCTTGGAAGGTCAAGATTCTGCCTTAGATGAATTAGAAGATAGCGACTTAGAC
AATCTTGAAATCTCAGATGGTGCAACCGAAGAACAAGAAGAACAAGAATCGACAGAGGACGCTACGAAAGATGCTCCTAT
AGTTCGCTTTATTAATAAGATCCTTTTAGATTCAATAAAAAATGGGGCTTCAGATATTCACTTTGAACCCTATGAAAAAA
GCTATCGTATCAGGAGCAGGATCGATGGAATTCTGCAGGAAGTTGCAAAACCGCCATCAAACCTAGCAGCGCGATTATCT
GCGCGCTTAAAAGTCATGTCACAAATGGATATTTCTGAAAAACGCGTGCCGCAAGATGGCCGTATTAAGATGAAGATATC
TAAAAAGCGCGCCATCGACTTCCGTGTAAATACACTCCCTACTCTTTGGGGAGAAAAAATCGTACTACGTATTCTAGATC
CAAGTAGTGCAAAAATGGGGGTCGAAGCTCTAGGCTTTGCGGAGTTCCAAAAAGAGCTTTACTTAGGCACACTCCACAGA
CCACAAGGTATGGTACTTGTAACCGGGCCAACTGGTAGTGGTAAAACCGTAAGCCTTTATACCGGTCTGAATATCCTTAA
TACCGAAGAACGAAATATCTCTACTGCGGAAGACCCAGTAGAGATCAACCTAGAGGGTATAAACCAGGTTCATGTAAACG
CTAAAGTCGGTTTAAATTTCGCAGAGGCTTTACGCGCCTTCCTACGTCAAGACCCGGACGTAGTTATGGTGGGTGAGATT
CGAGACCTCGAAACAGCAGAAATAGCTATTAAAGCGGCCCAAACAGGTCATATGGTTTTATCCACCCTCCATACAAATAG
CGCACCTGAAACACTTACCCGTCTACGTAACATGGGCGTACCCGCCTTTAATATTGCTACATCTGTAAGTCTCATCATTG
CACAACGCTTAGGGCGGCGTTTATGTGAAAGCTGTAAGAAACCCATTACACTTCCTGAAGTTACCTTAAAGGAAGAGGGC
TTTACTGATGAAGAGATCAACGACCTTACTCTTTTCGAAGCCAATCAAGATGGCTGCAGCAAATGCAATAGAGGCTATAA
AGGCCGAGTAGGTATCTATGAAATGTTACCTATGACTCCAGAAATTGCAGAAGTAATTATGGAGAATGGCAACTCTCTGG
ATATACTTAGAGTAGCTGAAACACAAAACTTCCAGAGCTTACGCCGCTCTGGGTTAAAGAAAGTCGCGGAAGGAATCACC
AGTCTTGAAGAGATGAACCGAGTCATCAAGACCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.469

98.949

0.588
  pilB Acinetobacter baumannii D1279779

58.494

100

0.585
  pilB Legionella pneumophila strain ERS1305867

54.77

99.124

0.543
  pilB Vibrio cholerae strain A1552

55.513

90.543

0.503
  pilF Neisseria gonorrhoeae MS11

49.554

98.249

0.487
  pilB Vibrio campbellii strain DS40M4

53.682

90.368

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

53.606

89.842

0.482

Multiple sequence alignment