Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMG4O_RS14560 Genome accession   NZ_CP179934
Coordinates   3106493..3108214 (+) Length   573 a.a.
NCBI ID   WP_414434048.1    Uniprot ID   -
Organism   Alloalcanivorax venustensis strain A6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3101493..3113214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMG4O_RS14540 (ACMG4O_14540) nadC 3102818..3103663 (+) 846 WP_414434045.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACMG4O_RS14545 (ACMG4O_14545) - 3103788..3104729 (+) 942 WP_414434046.1 DUF4351 domain-containing protein -
  ACMG4O_RS14550 (ACMG4O_14550) - 3105123..3105482 (+) 360 WP_370185368.1 glycine zipper family protein -
  ACMG4O_RS14555 (ACMG4O_14555) pilA 3105734..3106171 (-) 438 WP_414434047.1 pilin Machinery gene
  ACMG4O_RS14560 (ACMG4O_14560) pilB 3106493..3108214 (+) 1722 WP_414434048.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMG4O_RS14565 (ACMG4O_14565) pilC 3108242..3109465 (+) 1224 WP_414434049.1 type II secretion system F family protein Machinery gene
  ACMG4O_RS14570 (ACMG4O_14570) pilD 3109468..3110352 (+) 885 WP_414434050.1 prepilin peptidase Machinery gene
  ACMG4O_RS14575 (ACMG4O_14575) coaE 3110476..3111078 (+) 603 WP_370209635.1 dephospho-CoA kinase -
  ACMG4O_RS14580 (ACMG4O_14580) - 3111038..3111265 (+) 228 WP_414434051.1 DNA gyrase inhibitor YacG -
  ACMG4O_RS14585 (ACMG4O_14585) mutT 3111363..3112367 (-) 1005 WP_414434052.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62665.56 Da        Isoelectric Point: 5.8423

>NTDB_id=1094016 ACMG4O_RS14560 WP_414434048.1 3106493..3108214(+) (pilB) [Alloalcanivorax venustensis strain A6]
MNSSAASLSGLARRLVKEGHLSMEAAQTAAAEARQARVALVHHLVAGGHVSAAVVARSAAEEFGDPLIDLNAFSKEMMVK
DIVDPKLITKHTILPLLRRGSRLFVAVSDPTNLPALEEIRFNTGLNVETVIAEDDKLRRWIDQLESEQEGKAFDNLDDDL
DNLETADTDAVPSGGEDAAAGSDDAPIVRFVNKLLLDAIKGGASDLHFEPYEKTYRVRFRQDGILETVAKPPITTASKLA
ARLKVMARMDISERRVPQDGRIKLKISKSRAIDFRVNTCPTLYGEKIVLRILDAESAKMGIDHLGYEEDQKKMYMDALSK
PQGMILVTGPTGSGKTVSLYTGVNILNTPERNISTAEDPVEINLEGVNQVNVNPKQGMEFSTALRAFLRQDPDIILVGEI
RDLETAEIAVKAAQTGHLVLSTLHTNSAPETLTRLRNMGVPSFNIATSVILIIAQRLARRLCEHCKEPVDVPRQSLVEMG
FTDEDIQSGFTVYGPSGKGCEKCKGGYKGRVGVYEVVRITDGIARIIMEEGNSIQIADQCRKEGFNDLYRSGLIKVMHGV
TSLEEVSRVTSGH

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=1094016 ACMG4O_RS14560 WP_414434048.1 3106493..3108214(+) (pilB) [Alloalcanivorax venustensis strain A6]
ATGAATAGTTCCGCTGCATCTTTAAGCGGCCTGGCCCGCCGCCTTGTAAAGGAAGGCCACCTCTCCATGGAAGCGGCGCA
GACCGCCGCCGCCGAAGCGCGGCAGGCGCGGGTGGCGCTTGTTCATCATCTAGTGGCGGGCGGCCACGTCAGCGCCGCCG
TGGTGGCGCGCTCCGCCGCAGAGGAGTTCGGCGACCCGCTGATCGATCTGAACGCTTTCTCTAAAGAGATGATGGTCAAG
GACATCGTCGACCCCAAGTTGATCACCAAGCACACCATCCTGCCGCTGCTGCGGAGGGGCAGCCGCTTGTTCGTGGCGGT
GTCCGACCCCACCAACCTACCGGCTCTGGAAGAGATCCGCTTCAATACCGGCCTGAATGTGGAAACGGTAATCGCCGAGG
ACGACAAGCTGCGGCGCTGGATCGATCAGCTGGAGTCCGAGCAGGAAGGTAAGGCGTTCGACAATCTGGATGACGATCTG
GATAACCTGGAGACCGCCGATACCGATGCTGTGCCCAGTGGTGGCGAAGACGCAGCGGCAGGCTCTGACGATGCGCCCAT
CGTGCGCTTCGTGAACAAGCTGCTGCTGGACGCCATTAAAGGTGGCGCCTCGGACCTGCACTTCGAACCCTACGAAAAGA
CCTACCGGGTGCGGTTCCGTCAGGACGGTATTCTGGAGACGGTGGCCAAGCCACCGATCACCACCGCCAGCAAACTGGCC
GCCCGCCTTAAAGTGATGGCGCGCATGGACATTTCCGAGCGCCGGGTGCCCCAGGACGGCCGAATCAAGCTGAAGATTTC
CAAGAGCCGGGCTATCGACTTCCGTGTTAACACTTGCCCTACCCTCTATGGCGAGAAGATCGTGCTGCGGATACTGGACG
CGGAAAGCGCCAAAATGGGCATCGATCACCTGGGCTACGAGGAAGACCAGAAGAAGATGTATATGGATGCCCTGAGCAAG
CCTCAGGGCATGATTCTGGTTACCGGGCCTACCGGTTCGGGTAAGACCGTGTCTCTCTATACCGGCGTGAACATCCTCAA
TACGCCAGAACGGAACATCTCCACGGCGGAAGATCCGGTGGAGATCAACCTGGAGGGGGTCAACCAGGTCAATGTGAACC
CCAAGCAGGGTATGGAGTTTTCCACCGCCCTGCGTGCCTTCCTGCGCCAGGACCCGGATATCATTCTGGTGGGGGAAATC
CGGGATCTGGAGACCGCCGAGATCGCGGTGAAAGCAGCGCAGACCGGTCACTTGGTGCTTTCGACCCTGCACACCAACAG
CGCGCCGGAGACGCTGACCCGACTGCGCAATATGGGCGTGCCGTCGTTCAATATCGCCACTTCCGTGATTCTGATTATCG
CTCAGCGACTGGCCCGGCGGCTGTGCGAGCACTGCAAGGAGCCTGTGGATGTGCCCAGGCAATCGCTGGTTGAGATGGGT
TTCACGGACGAGGACATCCAATCCGGGTTTACTGTGTACGGCCCCAGTGGCAAAGGCTGCGAGAAGTGCAAAGGCGGCTA
CAAGGGGCGCGTGGGGGTTTACGAGGTAGTCCGCATCACCGACGGCATTGCGCGCATCATCATGGAGGAAGGTAATTCCA
TCCAGATCGCCGACCAGTGCCGCAAAGAGGGGTTCAACGATCTGTACCGTTCCGGCCTGATCAAGGTCATGCACGGCGTC
ACCAGTCTTGAGGAAGTGAGCCGGGTGACCAGCGGGCACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.713

99.825

0.616
  pilB Acinetobacter baylyi ADP1

59.895

99.651

0.597
  pilB Legionella pneumophila strain ERS1305867

53.054

100

0.531
  pilF Neisseria gonorrhoeae MS11

49.027

98.604

0.483
  pilB Vibrio cholerae strain A1552

52.827

89.529

0.473
  pilB Vibrio parahaemolyticus RIMD 2210633

46.996

98.778

0.464
  pilB Vibrio campbellii strain DS40M4

46.466

98.778

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

37.441

100

0.414

Multiple sequence alignment