Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACLUPT_RS25575 Genome accession   NZ_CP179718
Coordinates   5552982..5554211 (+) Length   409 a.a.
NCBI ID   WP_237675783.1    Uniprot ID   -
Organism   Variovorax sp. SCN45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5547982..5559211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLUPT_RS25545 (ACLUPT_25545) cgtA 5548071..5549147 (-) 1077 WP_237675780.1 Obg family GTPase CgtA -
  ACLUPT_RS25550 (ACLUPT_25550) rpmA 5549219..5549479 (-) 261 WP_081267930.1 50S ribosomal protein L27 -
  ACLUPT_RS25555 (ACLUPT_25555) rplU 5549492..5549803 (-) 312 WP_057592097.1 50S ribosomal protein L21 -
  ACLUPT_RS25560 (ACLUPT_25560) - 5549987..5550979 (+) 993 WP_237675781.1 polyprenyl synthetase family protein -
  ACLUPT_RS25570 (ACLUPT_25570) pilB 5551222..5552955 (+) 1734 WP_237675782.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLUPT_RS25575 (ACLUPT_25575) pilC 5552982..5554211 (+) 1230 WP_237675783.1 type II secretion system F family protein Machinery gene
  ACLUPT_RS25580 (ACLUPT_25580) - 5554211..5555176 (+) 966 WP_237675784.1 prepilin peptidase -
  ACLUPT_RS25585 (ACLUPT_25585) coaE 5555188..5555796 (+) 609 WP_237675785.1 dephospho-CoA kinase -
  ACLUPT_RS25590 (ACLUPT_25590) - 5555866..5557359 (+) 1494 WP_237675786.1 MBOAT family O-acyltransferase -
  ACLUPT_RS25595 (ACLUPT_25595) - 5557361..5558395 (+) 1035 WP_237675787.1 hypothetical protein -
  ACLUPT_RS25600 (ACLUPT_25600) zapD 5558426..5559181 (+) 756 WP_237675788.1 cell division protein ZapD -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44795.55 Da        Isoelectric Point: 9.7864

>NTDB_id=1092813 ACLUPT_RS25575 WP_237675783.1 5552982..5554211(+) (pilC) [Variovorax sp. SCN45]
MATVASSRSQPTHKEFVFEWEGKDRNGKLVRGELRAAGENQVQAALRRQGVLASKIKKRRMRSGKKIKPKDIAIFTRQLA
TMMKAGVPLMQSFDIVGRGNANASVAKLLNDIRSDVETGTSLSMAFRKFPKYFDNLYCNLVEAGEAAGILEELLDRLATY
MEKTEAIKSKIKSALMYPTSVIVVAFVVVAIIMIFVIPAFKQVFTSFGADLPAPTLFVMAISEFFVSYWWLIFGGLGGGI
YFFLQAWKRNERVQRVMDRALLRVPIFGVLIEKSCVARWTRTLATMFAAGIPLVEALDSVGGASGNTVYGDATAKIQQEV
STGTSLTAAMTNVNLFPSMVIQMTAIGEESGSIDHMLGKAADFYESEVDDMVAGLSSLMEPIIIVFLGTIIGGIVVSMYL
PIFKLGQVV

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=1092813 ACLUPT_RS25575 WP_237675783.1 5552982..5554211(+) (pilC) [Variovorax sp. SCN45]
ATGGCAACAGTGGCATCCTCCCGCTCCCAGCCCACGCACAAGGAATTTGTCTTCGAGTGGGAAGGCAAGGACCGCAACGG
CAAGCTGGTGCGCGGCGAACTTCGGGCCGCCGGCGAAAACCAGGTACAGGCTGCGTTGCGCCGCCAGGGCGTGCTCGCCT
CGAAGATCAAGAAGCGGCGGATGCGCTCGGGCAAGAAGATCAAGCCCAAGGACATCGCCATCTTCACGCGCCAGTTGGCG
ACGATGATGAAGGCCGGCGTGCCGCTGATGCAGTCCTTCGACATCGTCGGACGCGGCAACGCAAACGCGAGCGTGGCCAA
GCTGTTGAACGACATTCGCAGTGACGTGGAAACAGGTACGTCTCTTTCGATGGCCTTCCGCAAGTTTCCGAAGTACTTCG
ACAACCTCTACTGCAACCTGGTGGAGGCCGGTGAGGCCGCCGGTATCCTGGAAGAGTTGCTGGACCGCCTTGCCACGTAC
ATGGAAAAAACCGAGGCGATCAAGTCCAAGATCAAGTCGGCGCTGATGTACCCGACTTCGGTGATCGTGGTGGCGTTCGT
GGTGGTAGCCATCATCATGATCTTCGTGATACCGGCGTTCAAGCAGGTGTTCACCTCGTTCGGCGCCGACCTGCCGGCAC
CCACGCTGTTCGTGATGGCGATCAGCGAGTTCTTCGTGTCGTACTGGTGGCTGATCTTCGGCGGCCTCGGCGGCGGCATC
TACTTCTTCCTGCAAGCCTGGAAGCGCAACGAACGCGTGCAGAGAGTCATGGACCGGGCACTGCTGAGAGTGCCGATTTT
CGGCGTGCTGATCGAGAAGTCCTGCGTGGCTCGCTGGACCCGTACGCTGGCAACCATGTTTGCTGCAGGCATACCGCTGG
TGGAAGCGCTCGACTCCGTGGGCGGTGCCTCCGGCAATACCGTCTACGGAGATGCCACAGCCAAGATTCAGCAGGAAGTC
TCGACCGGCACCAGCCTCACGGCAGCCATGACCAACGTCAATCTCTTCCCCTCGATGGTCATCCAGATGACCGCCATCGG
CGAAGAATCCGGCTCCATCGACCACATGCTGGGCAAGGCCGCCGACTTCTACGAATCCGAAGTGGACGACATGGTCGCGG
GCCTCTCGAGCCTGATGGAACCCATCATCATCGTGTTCCTCGGCACCATCATCGGCGGCATCGTCGTCTCGATGTACCTG
CCCATCTTCAAGCTCGGCCAGGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

52.97

98.778

0.523
  pilG Neisseria gonorrhoeae MS11

53.149

97.066

0.516
  pilG Neisseria meningitidis 44/76-A

52.897

97.066

0.513
  pilC Acinetobacter baylyi ADP1

51.508

97.311

0.501
  pilC Legionella pneumophila strain ERS1305867

49.749

97.311

0.484
  pilC Acinetobacter baumannii D1279779

49.37

97.066

0.479
  pilC Vibrio cholerae strain A1552

41.457

97.311

0.403
  pilC Vibrio campbellii strain DS40M4

39.702

98.533

0.391
  pilC Thermus thermophilus HB27

37.47

100

0.384

Multiple sequence alignment