Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLUPT_RS25570 Genome accession   NZ_CP179718
Coordinates   5551222..5552955 (+) Length   577 a.a.
NCBI ID   WP_237675782.1    Uniprot ID   -
Organism   Variovorax sp. SCN45     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5546222..5557955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLUPT_RS25535 (ACLUPT_25535) - 5546223..5546813 (+) 591 WP_237675778.1 CNP1-like family protein -
  ACLUPT_RS25540 (ACLUPT_25540) proB 5546857..5547996 (-) 1140 WP_237675779.1 glutamate 5-kinase -
  ACLUPT_RS25545 (ACLUPT_25545) cgtA 5548071..5549147 (-) 1077 WP_237675780.1 Obg family GTPase CgtA -
  ACLUPT_RS25550 (ACLUPT_25550) rpmA 5549219..5549479 (-) 261 WP_081267930.1 50S ribosomal protein L27 -
  ACLUPT_RS25555 (ACLUPT_25555) rplU 5549492..5549803 (-) 312 WP_057592097.1 50S ribosomal protein L21 -
  ACLUPT_RS25560 (ACLUPT_25560) - 5549987..5550979 (+) 993 WP_237675781.1 polyprenyl synthetase family protein -
  ACLUPT_RS25570 (ACLUPT_25570) pilB 5551222..5552955 (+) 1734 WP_237675782.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLUPT_RS25575 (ACLUPT_25575) pilC 5552982..5554211 (+) 1230 WP_237675783.1 type II secretion system F family protein Machinery gene
  ACLUPT_RS25580 (ACLUPT_25580) - 5554211..5555176 (+) 966 WP_237675784.1 prepilin peptidase -
  ACLUPT_RS25585 (ACLUPT_25585) coaE 5555188..5555796 (+) 609 WP_237675785.1 dephospho-CoA kinase -
  ACLUPT_RS25590 (ACLUPT_25590) - 5555866..5557359 (+) 1494 WP_237675786.1 MBOAT family O-acyltransferase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62484.63 Da        Isoelectric Point: 5.5901

>NTDB_id=1092812 ACLUPT_RS25570 WP_237675782.1 5551222..5552955(+) (pilB) [Variovorax sp. SCN45]
MAAAELPVKESAQIALPGLARALVSAGKLPAKTAEDIYQKSLSGRTSFIAELTGTGAVSAADLAHTLSSAFGAPLLDLDA
IDHQRLPKDLLDPKLCLAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMIEAAAVSAAETI
NSIVGSEFEFDEVTADTSGDANEQAIAEVEDAPVVRFLHKMLLDAVGMRASDIHFEPYEHNYRVRFRVDGELREIASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARLGIDALGYDADEKAR
LLHAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNERAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCTVCRAPADVPR
EALLDAGFQEEQLDGSWKPYRPVGCSACSGGYKGRVGIYQVMPISEAIQEIILRDGSALDIAQQSEREGVRSLRQSGLRK
VMQGLTSLEEVVACTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1092812 ACLUPT_RS25570 WP_237675782.1 5551222..5552955(+) (pilB) [Variovorax sp. SCN45]
ATGGCCGCTGCCGAACTTCCAGTCAAAGAATCCGCGCAGATAGCGCTTCCAGGGCTTGCGCGTGCCCTGGTTTCCGCCGG
CAAGCTGCCCGCCAAGACGGCGGAAGACATCTATCAAAAGTCGCTGAGTGGCCGCACCAGTTTCATCGCGGAACTGACCG
GCACGGGAGCCGTATCGGCCGCGGACCTTGCCCATACCCTTTCCAGCGCGTTCGGCGCTCCGCTGCTCGATCTCGACGCC
ATCGACCACCAGCGCCTGCCCAAGGATCTGCTTGACCCGAAGCTCTGCCTTGCCTACCGCATCGTCGTTCTCAGCAAGCG
CAATAACCGGCTGATCGTCGCCACGGCCGATCCGTCGGACCAGCAGGCGGCGGAAAAGATCAAGTTCGCCTCGCAGATGG
GCGTGGACTGGGTCATCGCGGAATACGACAAGCTGTCGCGCATGATCGAAGCCGCCGCCGTGAGCGCGGCAGAGACCATC
AACAGCATCGTCGGCAGCGAGTTCGAGTTCGACGAAGTCACGGCCGACACCTCGGGCGATGCCAACGAGCAGGCGATCGC
CGAAGTCGAAGACGCACCGGTCGTGCGTTTTCTGCACAAGATGCTCCTCGACGCCGTGGGGATGCGGGCGTCGGACATCC
ACTTCGAGCCCTACGAGCACAACTACCGCGTGCGCTTTCGCGTCGACGGCGAACTGCGCGAGATCGCCAGCCCGCCGACC
GTCATCAAGGACAAGCTCGCCTCCCGCATCAAGGTGATTTCTCGGCTCGACATCTCCGAGAAGCGCGTGCCGCAGGACGG
CCGCATGAAGCTCAAGATCGGCCCCGACCGCGTGATCGACTTTCGCGTGAGCACGCTGCCCACGCTGTTCGGCGAGAAGA
TCGTCGTTCGTATTCTCGACCCCAGCAGCGCGCGCCTGGGCATCGATGCACTGGGCTACGACGCCGACGAGAAGGCACGG
CTGCTGCACGCCATCGGCCGTCCCTACGGCATGGTGCTAGTGACCGGCCCGACGGGTTCGGGCAAGACCGTGTCGCTCTA
CACCTGCCTGAACCTGCTGAACCAGCCGGGCGTGAACATCGCGACGGCGGAAGACCCGTCGGAAATCAACCTGCCCGGCG
TCAACCAGGTCAACGTCAACGAGCGTGCGGGCCTGACTTTCGCGGCCGCGCTGCGTGCGTTCCTGCGGCAGGATCCCGAC
ATCATCATGGTCGGTGAAATCCGCGACCTCGAGACCGCCGACATTTCGATCAAGGCCGCCCAGACGGGCCACCTGGTGCT
CTCGACACTGCACACCAACGACGCGCCGACCACGCTCACGCGGATGCGCAACATGGGCATCGCGCCGTTCAACATCGCAT
CGAGCGTGATCCTGATCACCGCGCAGCGCCTTGCGCGGCGCCTGTGCACCGTCTGCCGTGCGCCCGCGGACGTGCCGCGC
GAGGCACTGCTCGACGCAGGATTCCAGGAAGAACAGCTGGACGGCAGCTGGAAGCCCTACCGCCCCGTCGGTTGTTCGGC
ATGCAGCGGCGGCTACAAGGGCCGCGTCGGCATCTACCAGGTGATGCCGATCTCGGAGGCCATCCAGGAAATCATCCTGC
GCGACGGCAGCGCGCTCGACATCGCACAGCAGTCCGAGAGGGAAGGCGTGCGTTCGCTGCGCCAATCCGGCCTGCGCAAG
GTCATGCAGGGCCTCACTTCGCTCGAAGAAGTCGTGGCCTGCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.46

100

0.536
  pilB Acinetobacter baumannii D1279779

54.545

97.227

0.53
  pilF Neisseria gonorrhoeae MS11

52.931

97.574

0.516
  pilB Legionella pneumophila strain ERS1305867

48.571

97.054

0.471
  pilB Vibrio cholerae strain A1552

46.726

97.92

0.458
  pilB Vibrio campbellii strain DS40M4

45.907

97.4

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

45.796

96.88

0.444
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.884

94.107

0.385
  pilF Thermus thermophilus HB27

37.828

98.96

0.374

Multiple sequence alignment