Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACKW6E_RS13255 Genome accession   NZ_CP178915
Coordinates   2909084..2910343 (+) Length   419 a.a.
NCBI ID   WP_413975142.1    Uniprot ID   -
Organism   Stenotrophomonas acidaminiphila strain ZCMU-Sa01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2904084..2915343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKW6E_RS13230 (ACKW6E_13230) pilB 2904094..2905824 (-) 1731 WP_054667226.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACKW6E_RS13235 (ACKW6E_13235) - 2906039..2906737 (+) 699 WP_413975138.1 hypothetical protein -
  ACKW6E_RS13240 (ACKW6E_13240) galE 2906759..2907733 (-) 975 WP_413975139.1 UDP-glucose 4-epimerase GalE -
  ACKW6E_RS13245 (ACKW6E_13245) - 2907874..2908239 (-) 366 WP_413975140.1 pilin -
  ACKW6E_RS13250 (ACKW6E_13250) - 2908348..2908704 (-) 357 WP_413975141.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACKW6E_RS13255 (ACKW6E_13255) pilC 2909084..2910343 (+) 1260 WP_413975142.1 type II secretion system F family protein Machinery gene
  ACKW6E_RS13260 (ACKW6E_13260) pilD 2910351..2911214 (+) 864 WP_413975143.1 prepilin peptidase Machinery gene
  ACKW6E_RS13265 (ACKW6E_13265) coaE 2911261..2911884 (+) 624 WP_182212757.1 dephospho-CoA kinase -
  ACKW6E_RS13270 (ACKW6E_13270) - 2911915..2913252 (-) 1338 WP_413975144.1 sensor histidine kinase -
  ACKW6E_RS13275 (ACKW6E_13275) - 2913245..2913922 (-) 678 WP_056931224.1 response regulator transcription factor -
  ACKW6E_RS13280 (ACKW6E_13280) - 2914052..2914516 (-) 465 WP_192301407.1 hypothetical protein -

Sequence

Protein


Download         Length: 419 a.a.        Molecular weight: 45821.09 Da        Isoelectric Point: 10.0321

>NTDB_id=1092444 ACKW6E_RS13255 WP_413975142.1 2909084..2910343(+) (pilC) [Stenotrophomonas acidaminiphila strain ZCMU-Sa01]
MSVTRSAVSKPPMERSTSLMQPFVWEGTDKRGVKMKGEQEARNANLLRAELRKQGITPTVVKPKPKPLFGAAGKPIKPRD
IAFFSRQMATMMKSGVPIVSALEIIGSGHKNPRMAKLVGQVRGDLEGGSSLYEAISKHPVQFDELYRNLVRAGEGAGVLE
TVLETIANYQENLETLKGKIKKALFYPTMVIAVALIVSAVLLIWVVPQFEEIFKSFGTDLPVFTQMIVAASDFMVGWWWL
ILLVGIGAIGGSSFAYKRSPRMQHALDRLILKVPVIGQIMHNSSIARFARTTAVTFKAGVPLVEALGIVAGATGNKIYEE
AVFRMRDDVAVGYPVNMAMKQVNLFPHMVIQMTAIGEEAGALDTMLFKVAEYFEQEVNNAVDALSSLIEPLIMVFIGIVV
GSMVIGMYLPIFKLASTVG

Nucleotide


Download         Length: 1260 bp        

>NTDB_id=1092444 ACKW6E_RS13255 WP_413975142.1 2909084..2910343(+) (pilC) [Stenotrophomonas acidaminiphila strain ZCMU-Sa01]
ATGTCCGTTACCCGAAGTGCAGTAAGCAAGCCGCCCATGGAACGCAGCACCAGCCTGATGCAGCCATTTGTATGGGAGGG
GACCGACAAACGCGGCGTCAAGATGAAGGGCGAACAGGAAGCCCGCAACGCCAACCTGTTGCGTGCCGAACTGCGTAAGC
AGGGCATCACGCCCACGGTCGTCAAGCCCAAGCCCAAGCCACTGTTTGGTGCCGCCGGCAAGCCCATCAAGCCGCGGGAC
ATTGCCTTCTTCAGCCGTCAAATGGCAACAATGATGAAATCAGGCGTCCCCATTGTGAGCGCGCTTGAGATCATCGGGAG
TGGCCACAAGAATCCGCGCATGGCCAAGCTGGTGGGGCAGGTCCGTGGCGATCTGGAAGGCGGCTCCTCACTCTACGAGG
CCATCAGCAAGCACCCGGTCCAGTTCGACGAGCTGTACCGCAACCTGGTGCGGGCCGGCGAGGGCGCCGGCGTGCTGGAG
ACGGTGCTTGAGACCATCGCGAACTACCAGGAAAACCTGGAAACTCTGAAAGGCAAGATCAAGAAGGCATTGTTCTACCC
GACCATGGTCATTGCTGTCGCACTGATCGTCAGCGCGGTTCTTCTGATCTGGGTCGTGCCGCAGTTCGAGGAGATATTCA
AGAGCTTCGGCACCGACCTCCCGGTGTTCACCCAGATGATCGTCGCGGCCTCCGATTTCATGGTTGGCTGGTGGTGGTTG
ATCCTGCTTGTCGGAATCGGCGCCATAGGCGGCAGCAGTTTCGCCTACAAGCGCTCACCGAGAATGCAACACGCGCTGGA
CCGATTGATCCTCAAGGTTCCGGTGATCGGTCAGATCATGCACAACAGTTCCATCGCCCGTTTCGCACGCACGACGGCGG
TGACGTTCAAGGCAGGCGTGCCGCTGGTCGAAGCACTGGGCATCGTCGCCGGCGCCACCGGCAACAAGATCTATGAAGAG
GCTGTCTTTCGCATGCGCGACGACGTGGCGGTAGGCTATCCGGTAAACATGGCCATGAAACAGGTCAACCTGTTTCCGCA
CATGGTGATCCAGATGACCGCCATCGGCGAGGAAGCCGGCGCGCTGGACACCATGCTGTTCAAGGTGGCCGAGTACTTCG
AGCAGGAAGTGAACAATGCCGTCGACGCCTTGAGCAGCCTGATCGAACCACTGATCATGGTGTTCATCGGCATCGTGGTC
GGCAGCATGGTGATCGGCATGTACCTGCCCATCTTCAAGCTCGCCTCCACCGTTGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(288-410)

Type II secretion system (T2SS), protein F (T2SSF)

(84-207)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

50.732

97.852

0.496
  pilC Acinetobacter baylyi ADP1

49.877

96.659

0.482
  pilC Pseudomonas stutzeri DSM 10701

50.758

94.511

0.48
  pilC Acinetobacter baumannii D1279779

48.395

96.659

0.468
  pilG Neisseria gonorrhoeae MS11

43

95.465

0.411
  pilG Neisseria meningitidis 44/76-A

41.728

96.659

0.403
  pilC Vibrio cholerae strain A1552

40

95.465

0.382
  pilC Thermus thermophilus HB27

38.235

97.375

0.372
  pilC Vibrio campbellii strain DS40M4

38.75

95.465

0.37

Multiple sequence alignment