Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACMDB8_RS23675 Genome accession   NZ_CP178857
Coordinates   5283291..5284508 (+) Length   405 a.a.
NCBI ID   WP_409078806.1    Uniprot ID   -
Organism   Pseudomonas sp. PLb12A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5278291..5289508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMDB8_RS23660 (ACMDB8_23660) - 5278723..5281713 (+) 2991 WP_223541984.1 DUF748 domain-containing protein -
  ACMDB8_RS23665 (ACMDB8_23665) - 5281985..5282341 (-) 357 WP_056728649.1 BON domain-containing protein -
  ACMDB8_RS23670 (ACMDB8_23670) - 5282642..5283064 (-) 423 WP_056728651.1 pilin -
  ACMDB8_RS23675 (ACMDB8_23675) pilC 5283291..5284508 (+) 1218 WP_409078806.1 type II secretion system F family protein Machinery gene
  ACMDB8_RS23680 (ACMDB8_23680) pilD 5284510..5285382 (+) 873 WP_056728655.1 prepilin peptidase Machinery gene
  ACMDB8_RS23685 (ACMDB8_23685) coaE 5285379..5286002 (+) 624 WP_046041800.1 dephospho-CoA kinase -
  ACMDB8_RS23690 (ACMDB8_23690) yacG 5285999..5286199 (+) 201 WP_007970539.1 DNA gyrase inhibitor YacG -
  ACMDB8_RS23695 (ACMDB8_23695) - 5286235..5286924 (-) 690 WP_409078841.1 energy-coupling factor ABC transporter permease -
  ACMDB8_RS23700 (ACMDB8_23700) - 5286996..5287118 (-) 123 Protein_4657 LysR family transcriptional regulator -
  ACMDB8_RS23705 (ACMDB8_23705) - 5287229..5287471 (+) 243 WP_056728659.1 DUF2790 domain-containing protein -
  ACMDB8_RS23710 (ACMDB8_23710) - 5287481..5288749 (-) 1269 WP_223541985.1 OprD family porin -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44192.12 Da        Isoelectric Point: 10.1117

>NTDB_id=1091963 ACMDB8_RS23675 WP_409078806.1 5283291..5284508(+) (pilC) [Pseudomonas sp. PLb12A]
MAVKAAKISVYAWEGIDRKGTKVTGELSAQNPALIKAKLRKQGISPGKVRKKNPSVFDMGKRIKAQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPAMRKLVDELKQEVAAGSSFAATLRKKPRYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ESLKARIRKAMTYPLVVVFVAAIVTGILLVNVVPQFESVFKGFGAELPAFTVMVIGLSQFMQAWWWVVLGALVTTAFGVR
HALRTSPAFRDRMDTWLLKLPLVGTLMHKSAVARFARTLSTTFAAGVPLVEALESVAGATGNVVFKHAVLRIRQDISTGM
QLHFAMRTCGVFPNMAIQMTAIGEESGTLDAMLDKVAGFYEDNVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1091963 ACMDB8_RS23675 WP_409078806.1 5283291..5284508(+) (pilC) [Pseudomonas sp. PLb12A]
ATGGCGGTCAAGGCAGCGAAAATCAGCGTGTATGCATGGGAAGGCATCGACCGCAAAGGCACAAAAGTGACCGGCGAACT
CAGCGCTCAAAACCCGGCGCTGATCAAGGCGAAACTGCGCAAGCAAGGCATCAGTCCCGGCAAGGTGCGCAAGAAAAACC
CTTCGGTTTTCGACATGGGCAAGCGCATCAAGGCCCAGGACATTGCCCTGTTCACCCGGCAAATGGCGACGATGATGAAG
GCCGGCGTGCCGTTGTTGCAGTCGTTCGACATCATCGGCGAAGGCTTCGACAATCCGGCCATGCGCAAGCTGGTGGACGA
ACTGAAACAGGAAGTTGCGGCAGGCAGCAGTTTCGCCGCCACCCTGCGCAAGAAGCCCCGGTATTTCGATGAGCTGTATT
GCAACCTGGTGGATGCCGGCGAGCAGGCCGGTGCCCTCGATACCCTTTTGGAGCGGGTCGCAACCTACAAGGAAAAAAGC
GAAAGCCTCAAGGCCAGGATCAGGAAAGCCATGACCTACCCGCTGGTGGTGGTGTTCGTCGCGGCGATTGTCACCGGGAT
TCTGCTGGTCAATGTCGTGCCGCAGTTCGAATCCGTGTTCAAAGGCTTTGGCGCCGAACTGCCGGCCTTCACCGTGATGG
TCATTGGCCTGTCGCAATTCATGCAGGCCTGGTGGTGGGTGGTCCTTGGCGCGCTGGTCACAACGGCCTTCGGCGTGCGC
CACGCCCTCAGGACATCCCCTGCCTTCCGTGACCGGATGGACACCTGGCTGCTGAAACTGCCGCTGGTGGGCACCCTGAT
GCACAAGTCCGCCGTGGCCCGGTTCGCCCGTACACTGTCGACCACTTTCGCTGCCGGCGTGCCGCTGGTGGAGGCCCTGG
AATCAGTGGCCGGTGCGACCGGCAATGTCGTGTTCAAGCACGCGGTATTGCGCATCCGGCAGGACATCTCGACCGGCATG
CAATTGCACTTCGCCATGCGCACCTGCGGCGTCTTTCCGAACATGGCGATACAAATGACTGCAATCGGCGAAGAGTCCGG
CACCCTGGACGCTATGCTCGACAAAGTCGCGGGGTTCTACGAAGACAACGTCGATAACATGGTCGATAACCTCACCAGTC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGTGTTATCGTCGGTGGACTGGTAGTTGCCATGTACCTGCCGATCTTCCAA
CTTGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

70.864

100

0.709
  pilC Acinetobacter baumannii D1279779

56.618

100

0.57
  pilC Acinetobacter baylyi ADP1

56.373

100

0.568
  pilC Legionella pneumophila strain ERS1305867

53.535

97.778

0.523
  pilG Neisseria gonorrhoeae MS11

42.289

99.259

0.42
  pilC Vibrio cholerae strain A1552

42.929

97.778

0.42
  pilG Neisseria meningitidis 44/76-A

41.975

100

0.42
  pilC Vibrio campbellii strain DS40M4

40.887

100

0.41
  pilC Thermus thermophilus HB27

37.845

98.519

0.373

Multiple sequence alignment