Detailed information NTG1091964

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACMDB8_RS23680
• Genome accession: NZ_CP178857
• Coordinates: 5284510..5285382 (+)
• Length: 290 a.a.
• NCBI ID: WP_056728655.1
• Uniprot ID: A0A0Q8HGR7
• Organism: Pseudomonas sp. PLb12A
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5279510..5290382

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACMDB8_RS23665 (ACMDB8_23665)	-	5281985..5282341 (-)	357	WP_056728649.1	BON domain-containing protein	-
ACMDB8_RS23670 (ACMDB8_23670)	-	5282642..5283064 (-)	423	WP_056728651.1	pilin	-
ACMDB8_RS23675 (ACMDB8_23675)	pilC	5283291..5284508 (+)	1218	WP_409078806.1	type II secretion system F family protein	Machinery gene
ACMDB8_RS23680 (ACMDB8_23680)	pilD	5284510..5285382 (+)	873	WP_056728655.1	prepilin peptidase	Machinery gene
ACMDB8_RS23685 (ACMDB8_23685)	coaE	5285379..5286002 (+)	624	WP_046041800.1	dephospho-CoA kinase	-
ACMDB8_RS23690 (ACMDB8_23690)	yacG	5285999..5286199 (+)	201	WP_007970539.1	DNA gyrase inhibitor YacG	-
ACMDB8_RS23695 (ACMDB8_23695)	-	5286235..5286924 (-)	690	WP_409078841.1	energy-coupling factor ABC transporter permease	-
ACMDB8_RS23700 (ACMDB8_23700)	-	5286996..5287118 (-)	123	Protein_4657	LysR family transcriptional regulator	-
ACMDB8_RS23705 (ACMDB8_23705)	-	5287229..5287471 (+)	243	WP_056728659.1	DUF2790 domain-containing protein	-
ACMDB8_RS23710 (ACMDB8_23710)	-	5287481..5288749 (-)	1269	WP_223541985.1	OprD family porin	-
ACMDB8_RS23715 (ACMDB8_23715)	-	5288766..5289986 (-)	1221	WP_223541986.1	benzoate/H(+) symporter BenE family transporter	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32024.22 Da
• Isoelectric Point: 7.7490

>NTDB_id=1091964 ACMDB8_RS23680 WP_056728655.1 5284510..5285382(+) (pilD) [Pseudomonas sp. PLb12A]
MPLNEILIHSALAFVVLAGITGLLVGSFLNVVIWRLPKMLERDWREQAHDILGLPGETPLPTYNLLLPHSQCPHCGHRIR
AWENIPLLSYLFLRGRCSACAAAISPRYPLTELACGLLSAFVAWHFGFGWPAALVLILSWGLLAMSLIDAEHQILPDVLV
LPLIWLGLIVNSFGLFVSLHEALWGAIAGYGLLWSVFWLFKLVTGKDGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AIIGLILLRWRNAKTSTPIPFGPYLAIAGWIALLWGGQITDLYWQFVGLK

Nucleotide

• Length: 873 bp

>NTDB_id=1091964 ACMDB8_RS23680 WP_056728655.1 5284510..5285382(+) (pilD) [Pseudomonas sp. PLb12A]
ATGCCCTTGAATGAAATCCTGATTCACTCTGCGCTGGCCTTTGTCGTTCTCGCAGGCATCACCGGTCTGCTGGTCGGCAG
TTTTCTCAATGTCGTGATTTGGCGCCTGCCGAAAATGCTCGAACGCGACTGGCGCGAGCAGGCCCATGACATTCTCGGCC
TGCCTGGCGAAACGCCACTGCCGACTTACAACCTGTTGCTGCCCCACTCCCAGTGCCCGCACTGCGGTCACCGCATCCGC
GCCTGGGAAAACATCCCGCTGCTCAGTTATTTGTTTTTACGCGGCCGCTGCTCAGCCTGTGCGGCAGCGATCAGCCCGCG
CTATCCGTTGACCGAACTGGCCTGCGGTCTGTTGTCGGCGTTTGTGGCCTGGCACTTTGGCTTCGGTTGGCCAGCCGCCC
TGGTGCTGATCCTGAGCTGGGGGTTGCTGGCGATGAGCCTGATCGACGCCGAACATCAGATATTGCCGGACGTACTGGTG
CTGCCGTTGATATGGCTGGGGTTGATCGTCAACAGCTTCGGCCTGTTCGTGTCGTTGCACGAGGCGCTTTGGGGGGCGAT
TGCCGGCTACGGCCTGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTCACAGGCAAGGACGGCATGGGCTACGGCGATT
TCAAGCTGCTGGCGATGCTGGGCGCCTGGGGTGGCTGGCAGATTTTGCCCCTGACCATCCTGCTGTCCTCGCTGGTAGGT
GCCATTATCGGGCTGATACTGCTGCGCTGGCGCAACGCAAAAACCTCGACGCCGATCCCTTTCGGCCCCTATCTGGCCAT
TGCCGGCTGGATTGCGTTGCTCTGGGGTGGTCAAATAACCGACCTCTATTGGCAGTTTGTCGGTTTGAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: A0A0Q8HGR7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.678	94.138	0.524
pilD	Vibrio campbellii strain DS40M4	51.773	97.241	0.503
pilD	Acinetobacter baumannii D1279779	48.387	96.207	0.466
pilD	Acinetobacter nosocomialis M2	48.387	96.207	0.466
pilD	Neisseria gonorrhoeae MS11	51.163	88.966	0.455