Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACLRDN_RS01770 Genome accession   NZ_CP178608
Coordinates   381055..382194 (-) Length   379 a.a.
NCBI ID   WP_413458718.1    Uniprot ID   -
Organism   Herbaspirillum huttiense strain HhutSZ1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 376055..387194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLRDN_RS01740 (ACLRDN_01740) mutT 376602..377027 (+) 426 WP_413458714.1 8-oxo-dGTP diphosphatase MutT -
  ACLRDN_RS01745 (ACLRDN_01745) yacG 377070..377255 (-) 186 WP_034336666.1 DNA gyrase inhibitor YacG -
  ACLRDN_RS01750 (ACLRDN_01750) zapD 377292..378047 (-) 756 WP_088755872.1 cell division protein ZapD -
  ACLRDN_RS01755 (ACLRDN_01755) coaE 378261..378881 (-) 621 WP_413458715.1 dephospho-CoA kinase -
  ACLRDN_RS01760 (ACLRDN_01760) - 378886..379749 (-) 864 WP_413458716.1 prepilin peptidase -
  ACLRDN_RS01765 (ACLRDN_01765) - 379803..381068 (-) 1266 WP_413458717.1 type II secretion system F family protein -
  ACLRDN_RS01770 (ACLRDN_01770) pilF 381055..382194 (-) 1140 WP_413458718.1 GspE/PulE family protein Machinery gene
  ACLRDN_RS01775 (ACLRDN_01775) - 382345..383637 (-) 1293 WP_413458719.1 HlyC/CorC family transporter -
  ACLRDN_RS01780 (ACLRDN_01780) - 383811..384779 (-) 969 WP_413458720.1 2-hydroxyacid dehydrogenase -
  ACLRDN_RS01785 (ACLRDN_01785) - 384776..385633 (-) 858 WP_209533714.1 IclR family transcriptional regulator -
  ACLRDN_RS01790 (ACLRDN_01790) - 385900..386985 (+) 1086 WP_413458721.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 40798.45 Da        Isoelectric Point: 8.2654

>NTDB_id=1090423 ACLRDN_RS01770 WP_413458718.1 381055..382194(-) (pilF) [Herbaspirillum huttiense strain HhutSZ1]
MLHTPDTAVAEDDVPVVRLLHKLLADAVGMGASDLHFEPFEHVFRVRLRLDGVLHELAQPPLSLRDKLATRLKILARLDI
AEKRLPQDGKMRLALGARTVDFRVSTLPTQFGEKIVLRILDTAQAALSISDLGYEPAQQEALLQAISKPHGLVLMTGPTG
SGKTVSLYACLQRLNQPGVNIATAEDPVEINLPGINQVSINERAGLDFALALRAFLRQDPDVLMVGEIRDLETADIAVKA
SQTGHLVLSTLHTNDAPATLTRLLNMGVPAYNIAASVSLIVAQRLVRKLCRCRRPAGGTPATFVPHGCPACNQTGFRGRT
GIYQVMPVSAAMQSLILAQAGTLELGRQAQAEGIIDLRRAGLLKVLRGETSVGEILACT

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1090423 ACLRDN_RS01770 WP_413458718.1 381055..382194(-) (pilF) [Herbaspirillum huttiense strain HhutSZ1]
ATGCTGCACACTCCCGACACGGCCGTGGCCGAAGATGACGTTCCCGTGGTGCGCCTGTTGCACAAGCTGCTGGCCGATGC
GGTCGGCATGGGCGCGTCCGACCTGCATTTCGAACCCTTCGAACATGTCTTCCGCGTGCGCCTGCGCCTGGATGGCGTGC
TGCATGAGCTGGCGCAGCCGCCGCTGAGCTTGCGCGACAAGCTGGCCACGCGCCTGAAGATCCTGGCGCGGCTGGATATC
GCCGAAAAACGCCTGCCCCAGGATGGCAAGATGCGCCTGGCGCTGGGCGCGCGCACGGTGGATTTCCGTGTCTCGACGCT
GCCCACGCAGTTCGGTGAAAAGATCGTGCTGCGCATTCTCGATACTGCGCAGGCGGCTTTGTCGATCAGCGACCTCGGCT
ACGAACCGGCCCAGCAGGAAGCGCTGCTGCAAGCCATCAGCAAGCCGCACGGGCTGGTCCTGATGACCGGGCCGACCGGC
TCCGGCAAGACGGTCTCGCTGTATGCCTGCCTGCAGCGGCTGAACCAGCCCGGCGTGAACATCGCCACCGCCGAGGATCC
GGTGGAAATCAACCTGCCCGGCATCAACCAGGTCAGCATCAACGAACGCGCCGGACTGGACTTCGCCCTGGCGCTGCGCG
CCTTCCTGCGCCAGGATCCGGATGTGCTGATGGTGGGCGAGATCCGCGACCTGGAAACCGCCGACATCGCCGTCAAGGCT
TCGCAGACCGGGCACCTAGTGCTCTCCACCCTGCACACCAACGATGCCCCGGCCACGCTCACGCGGCTGCTCAACATGGG
CGTGCCGGCCTACAACATTGCGGCATCGGTCAGCCTGATCGTGGCCCAGCGGCTGGTGCGCAAGCTGTGCCGCTGCCGCC
GTCCGGCCGGTGGCACGCCGGCCACCTTCGTGCCGCACGGCTGCCCGGCCTGCAACCAGACCGGCTTTCGCGGGCGCACC
GGCATCTATCAGGTCATGCCGGTCAGCGCGGCCATGCAGTCGCTCATCCTGGCCCAGGCCGGCACATTGGAACTGGGCCG
GCAGGCGCAAGCAGAGGGCATCATCGACCTGCGGCGGGCGGGACTGCTGAAGGTCTTGCGCGGTGAAACCAGCGTGGGAG
AAATCCTTGCCTGTACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(23-288)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

56.709

100

0.591
  pilB Acinetobacter baylyi ADP1

55.325

100

0.562
  pilB Acinetobacter baumannii D1279779

54.381

100

0.557
  pilB Vibrio campbellii strain DS40M4

54.974

100

0.554
  pilB Vibrio parahaemolyticus RIMD 2210633

54.45

100

0.549
  pilB Vibrio cholerae strain A1552

54.047

100

0.546
  pilB Legionella pneumophila strain ERS1305867

51.282

100

0.528
  pilB Haemophilus influenzae Rd KW20

47.48

99.472

0.472
  pilB Haemophilus influenzae 86-028NP

47.215

99.472

0.47
  pilB/pilB1 Synechocystis sp. PCC 6803

42.118

100

0.451
  pilF Thermus thermophilus HB27

43.636

100

0.443
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.092

100

0.435
  pilB Glaesserella parasuis strain SC1401

49.371

83.905

0.414
  ctsE Campylobacter jejuni subsp. jejuni 81-176

39.37

100

0.396

Multiple sequence alignment