Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACL2DY_RS00330 Genome accession   NZ_CP178544
Coordinates   59481..60590 (-) Length   369 a.a.
NCBI ID   WP_087757149.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain JK     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 54481..65590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL2DY_RS00305 (ACL2DY_00305) - 54621..54851 (+) 231 WP_005320453.1 hypothetical protein -
  ACL2DY_RS00310 (ACL2DY_00310) brnQ 54927..56240 (-) 1314 WP_017412062.1 branched-chain amino acid transport system II carrier protein -
  ACL2DY_RS00315 (ACL2DY_00315) - 56426..57136 (-) 711 WP_413163839.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  ACL2DY_RS00320 (ACL2DY_00320) srmB 57264..58487 (+) 1224 WP_005320462.1 ATP-dependent RNA helicase SrmB -
  ACL2DY_RS00325 (ACL2DY_00325) yaaA 58676..59449 (-) 774 WP_087757148.1 peroxide stress protein YaaA -
  ACL2DY_RS00330 (ACL2DY_00330) pilU 59481..60590 (-) 1110 WP_087757149.1 type IVa pilus ATPase TapU Machinery gene
  ACL2DY_RS00335 (ACL2DY_00335) pilT 60626..61660 (-) 1035 WP_005320466.1 type IVa pilus ATPase TapT Machinery gene
  ACL2DY_RS00340 (ACL2DY_00340) - 61700..62401 (+) 702 WP_271773683.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACL2DY_RS00345 (ACL2DY_00345) proC 62492..63316 (+) 825 WP_271773682.1 pyrroline-5-carboxylate reductase -
  ACL2DY_RS00350 (ACL2DY_00350) - 63345..63896 (+) 552 WP_005320477.1 YggT family protein -
  ACL2DY_RS00355 (ACL2DY_00355) yggU 63896..64195 (+) 300 WP_005320479.1 DUF167 family protein YggU -
  ACL2DY_RS00360 (ACL2DY_00360) - 64214..64633 (+) 420 WP_005320482.1 DUF4426 domain-containing protein -
  ACL2DY_RS00365 (ACL2DY_00365) - 64702..65001 (-) 300 WP_011899099.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41059.10 Da        Isoelectric Point: 6.3717

>NTDB_id=1090051 ACL2DY_RS00330 WP_087757149.1 59481..60590(-) (pilU) [Aeromonas salmonicida strain JK]
MNLDALLAELVARKGSDLFITVGTPPTLKINGHLFSLGSEALDKQSALTLVRETLSPEHFERYIRTKEGNYAIHREALGR
FRVSAFWQQELPGMVVRRIETRIPTFDELTLPPILQEVAMAKRGLVLFVGATGTGKSTTQAAMIGYRNQNADGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQLLFDLSFNLRAIVAQQLVPSLDGRRRFAAFEVLLNTPLITDIIRKGEVHRLKEVMTKSTELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQMGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1090051 ACL2DY_RS00330 WP_087757149.1 59481..60590(-) (pilU) [Aeromonas salmonicida strain JK]
ATGAATCTGGATGCCCTGTTGGCCGAACTGGTCGCACGCAAAGGCTCGGATCTGTTCATCACGGTTGGTACGCCCCCCAC
CCTCAAGATCAACGGGCATCTGTTCTCCCTCGGCAGCGAAGCGCTCGACAAGCAGAGCGCGCTGACCCTGGTCAGGGAAA
CGCTCAGCCCTGAGCATTTCGAGCGATACATCCGCACCAAGGAGGGCAACTACGCCATCCACCGCGAGGCACTGGGACGC
TTTCGGGTAAGTGCCTTCTGGCAGCAGGAGTTGCCGGGCATGGTGGTACGCCGTATCGAGACCCGCATTCCAACCTTCGA
CGAACTGACCCTGCCGCCCATCTTGCAGGAGGTGGCCATGGCCAAACGCGGCCTGGTGCTGTTCGTCGGCGCCACCGGAA
CGGGCAAATCCACCACTCAGGCAGCCATGATCGGCTATCGAAACCAGAATGCGGATGGCCATATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGGGAAGTGGGCATAGATACGGAATCATTCGATGT
GGCGCTGAAGAGCTCCCTGCGCCAGGCACCTGACGTTATTCTCATCGGCGAGATCCGAAGCCAGGAAACCATGGAGTTCG
CACTGCAATTTGCCGAGACCGGCCATCTTTGTCTCGCGACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTACCGCAGGAGAAACACCGCCAGTTACTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
ACTGGTGCCAAGCCTGGATGGTCGCCGCCGCTTCGCGGCCTTTGAAGTGCTGCTCAACACGCCGCTCATCACGGATATCA
TCCGCAAAGGCGAGGTTCATCGTCTCAAGGAGGTGATGACCAAATCCACCGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTTACCCTGTTTTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCCGGCCGTGAGCAGATGGGTGCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.239

99.729

0.591
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

39.942

92.954

0.371
  pilT Legionella pneumophila strain ERS1305867

39.942

92.954

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36

Multiple sequence alignment