Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACKUFS_RS27205 Genome accession   NZ_CP178532
Coordinates   6042284..6043501 (-) Length   405 a.a.
NCBI ID   WP_007251659.1    Uniprot ID   A0A8T8BXM8
Organism   Pseudomonas cannabina strain YM7930     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 6034456..6048744 6042284..6043501 within 0


Gene organization within MGE regions


Location: 6034456..6048744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKUFS_RS27180 (ACKUFS_27180) tnpB 6038123..6038482 (+) 360 WP_007247726.1 IS66 family insertion sequence element accessory protein TnpB -
  ACKUFS_RS27185 (ACKUFS_27185) tnpC 6038499..6040031 (+) 1533 WP_007247725.1 IS66 family transposase -
  ACKUFS_RS27190 (ACKUFS_27190) yacG 6040584..6040793 (-) 210 WP_007251656.1 DNA gyrase inhibitor YacG -
  ACKUFS_RS27195 (ACKUFS_27195) coaE 6040790..6041413 (-) 624 WP_007251657.1 dephospho-CoA kinase -
  ACKUFS_RS27200 (ACKUFS_27200) pilD 6041410..6042282 (-) 873 WP_007251658.1 prepilin peptidase Machinery gene
  ACKUFS_RS27205 (ACKUFS_27205) pilC 6042284..6043501 (-) 1218 WP_007251659.1 type II secretion system F family protein Machinery gene
  ACKUFS_RS27210 (ACKUFS_27210) pilB 6043504..6045198 (-) 1695 WP_007251660.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACKUFS_RS27215 (ACKUFS_27215) - 6045424..6045813 (+) 390 WP_007251661.1 pilin -
  ACKUFS_RS27220 (ACKUFS_27220) - 6045978..6047285 (+) 1308 WP_080393985.1 O-antigen ligase family protein -
  ACKUFS_RS27225 (ACKUFS_27225) - 6047458..6048744 (+) 1287 WP_042913808.1 polysaccharide biosynthesis C-terminal domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44199.17 Da        Isoelectric Point: 10.1002

>NTDB_id=1089916 ACKUFS_RS27205 WP_007251659.1 6042284..6043501(-) (pilC) [Pseudomonas cannabina strain YM7930]
MASKAVKVSIYTWEGVDKKGAKTGGELSGHNLALVKAQLRKQGINPTKVREKSVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRALVGSLKQEVSAGNSFATALRKKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPTAVLIVAFIVSGILLIKVVPQFQSVFAGFNAELPAFTLMVIALSEVVQQWWLAIVVLFFVTFFMLK
RAYKRSQKFRDRLDRFALKIPIIGPLIFKSCVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVNQVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDAMLDKVATYYEEEVDNMVDSLTSLMEPMIMAVLGVIVGGLVIAMYLPIFK
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1089916 ACKUFS_RS27205 WP_007251659.1 6042284..6043501(-) (pilC) [Pseudomonas cannabina strain YM7930]
ATGGCCAGCAAGGCAGTCAAAGTCAGCATTTACACTTGGGAAGGTGTCGACAAGAAAGGCGCAAAAACCGGCGGAGAACT
GAGCGGCCATAATCTGGCACTTGTGAAGGCCCAACTGCGTAAGCAAGGCATCAATCCGACCAAGGTACGCGAAAAGTCAG
TTTCTATTTTTGGCAAGGGCAAGAAGATCAAGCCACTGGACATTGCGTTCTTTTCTCGTCAGATGGCGACGATGATGAAG
GCTGGCGTGCCCTTGCTACAGTCATTCGACATCATCAGTGAGGGCGCAGAGAATCCAAATATGCGTGCGCTGGTCGGCTC
ACTGAAACAGGAAGTTTCAGCCGGTAACAGCTTCGCAACGGCCTTACGGAAGAAGCCTGAATACTTCGATGATTTGTTTT
GCAATCTGGTGGATGCCGGTGAGCAAGCAGGCGCCCTTGAAAGCCTGCTCGACCGAGTAGCAAGCTATAAGGAAAAGACT
GAAAAGCTCAAAGCCAAAATCAAGAAAGCCATGACCTATCCGACTGCCGTATTGATTGTGGCTTTTATTGTCTCAGGCAT
TTTGCTGATTAAAGTAGTACCGCAGTTTCAATCAGTTTTTGCCGGATTTAACGCCGAACTACCTGCATTTACTCTGATGG
TTATTGCGCTTTCGGAGGTTGTCCAACAGTGGTGGCTGGCAATTGTCGTACTGTTTTTTGTGACTTTTTTCATGCTCAAG
AGAGCCTACAAACGATCTCAAAAATTTCGTGACCGACTTGATCGTTTTGCATTGAAAATCCCCATTATCGGACCGCTTAT
TTTCAAATCCTGCGTCGCGCGATACGCACGCACTCTCGCTACCACGTTTGCGGCGGGTGTTCCACTGGTTGAGGCGCTGG
ACTCGGTCGCCGGAGCGACTGGCAATGTGGTGTTCCGGAACGCAGTCAATCAAGTCAAACAGGATGTATCTACAGGCATG
CAGCTGAATTTCTCGATGCGCTCGACAGGCGTTTTTCCTAGCCTCGCTATACAGATGACCGCGATTGGGGAAGAGTCCGG
CGCACTTGACGCGATGCTTGACAAGGTAGCGACGTATTACGAAGAAGAAGTCGACAACATGGTTGATAGCCTGACCAGCC
TGATGGAACCGATGATCATGGCGGTATTGGGTGTCATCGTCGGTGGTCTGGTCATTGCGATGTATCTGCCAATTTTCAAA
CTTGGAAGCGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.827

100

0.738
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Acinetobacter baumannii D1279779

59.653

99.753

0.595
  pilC Legionella pneumophila strain ERS1305867

52.02

97.778

0.509
  pilG Neisseria gonorrhoeae MS11

42.432

99.506

0.422
  pilG Neisseria meningitidis 44/76-A

42.432

99.506

0.422
  pilC Vibrio campbellii strain DS40M4

41.379

100

0.415
  pilC Vibrio cholerae strain A1552

41.235

100

0.412
  pilC Thermus thermophilus HB27

38.155

99.012

0.378

Multiple sequence alignment