Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACLIJP_RS22205 Genome accession   NZ_CP177354
Coordinates   4974456..4975586 (+) Length   376 a.a.
NCBI ID   WP_110186684.1    Uniprot ID   -
Organism   Pokkaliibacter plantistimulans strain L1E11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4969456..4980586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLIJP_RS22180 (ACLIJP_22180) - 4969679..4970239 (+) 561 WP_110186680.1 SIS domain-containing protein -
  ACLIJP_RS22185 (ACLIJP_22185) - 4970261..4970662 (-) 402 WP_110186681.1 hypothetical protein -
  ACLIJP_RS22190 (ACLIJP_22190) - 4970802..4971389 (-) 588 WP_207780218.1 ATP-binding cassette domain-containing protein -
  ACLIJP_RS22195 (ACLIJP_22195) thiP 4971508..4973139 (-) 1632 WP_412053251.1 thiamine/thiamine pyrophosphate ABC transporter permease -
  ACLIJP_RS22200 (ACLIJP_22200) thiB 4973162..4974175 (-) 1014 WP_165838339.1 thiamine ABC transporter substrate binding subunit -
  ACLIJP_RS22205 (ACLIJP_22205) pilU 4974456..4975586 (+) 1131 WP_110186684.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLIJP_RS22210 (ACLIJP_22210) rfaH 4976368..4976874 (+) 507 WP_110186685.1 transcription/translation regulatory transformer protein RfaH -
  ACLIJP_RS22215 (ACLIJP_22215) - 4977025..4978089 (+) 1065 WP_110186686.1 undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase -
  ACLIJP_RS22220 (ACLIJP_22220) - 4978168..4978599 (+) 432 WP_133251267.1 hypothetical protein -
  ACLIJP_RS22225 (ACLIJP_22225) - 4978623..4980272 (+) 1650 WP_110186687.1 polysaccharide biosynthesis/export family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41981.17 Da        Isoelectric Point: 6.2894

>NTDB_id=1086824 ACLIJP_RS22205 WP_110186684.1 4974456..4975586(+) (pilU) [Pokkaliibacter plantistimulans strain L1E11]
MELVDLLRKMVDDDGSDLYLSPGAPPSIKFNGVLTAMADAVSPDEVKQLLFAVMSQPQIDEFQLQQELNFALSVPGVGRF
RVNLFIQRGQIGAVIRNIKTVIPQFDELGLPPVLREVILDKQGLVLFVGSTGSGKSTSLAALIDHRNSNHAGHIISIEDP
IEYLHRHKKSIINQREVGMDTHSYGEALKNTLRQAPDLILIGEIRDRDTMEYALAFAETGHLTLSTLHANNANQALDRIL
NFFPEDRHRQIQHDLSLNIRAFVSQRLLPTVDGKRCAAFEILLRTSSVSELIKRGEIGELKDIMAKSNHIGMMTFDQSIF
NLFKEGKITEETAMRNADSPNNMRLMIKFLKDGQGMADRKVPEFNTGGKPPAFEVE

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1086824 ACLIJP_RS22205 WP_110186684.1 4974456..4975586(+) (pilU) [Pokkaliibacter plantistimulans strain L1E11]
ATGGAACTTGTCGATTTACTACGCAAAATGGTCGATGACGACGGCTCAGACCTTTACCTGTCTCCAGGCGCACCTCCGAG
CATCAAATTCAACGGCGTTCTCACTGCGATGGCCGATGCGGTCAGCCCTGATGAAGTTAAACAGCTACTTTTTGCGGTGA
TGTCTCAACCTCAAATTGACGAATTCCAGTTACAGCAGGAATTGAACTTTGCTCTGTCAGTACCCGGAGTCGGACGCTTC
AGGGTCAACCTCTTTATTCAGCGTGGTCAGATTGGCGCGGTGATACGTAATATCAAGACTGTCATTCCTCAGTTTGACGA
ACTGGGCCTTCCACCAGTATTGCGCGAAGTCATTCTGGACAAACAAGGCCTGGTACTATTTGTCGGTTCCACTGGCTCGG
GTAAATCGACCTCTCTGGCAGCACTAATTGATCACCGCAACAGTAATCACGCTGGTCATATCATCTCCATTGAGGATCCG
ATCGAATACCTGCACCGCCACAAAAAATCGATCATCAACCAGCGTGAAGTGGGCATGGATACCCATAGTTACGGCGAGGC
GCTAAAGAATACACTGCGACAAGCGCCAGATCTCATATTGATCGGTGAGATCCGTGACCGTGACACGATGGAATATGCGC
TGGCCTTTGCCGAAACGGGCCACCTTACACTGTCGACGTTGCACGCCAATAACGCCAACCAGGCACTGGACCGCATTCTC
AACTTCTTCCCTGAGGACCGCCACCGCCAGATTCAGCACGACCTTTCATTGAATATTCGCGCCTTCGTGTCACAGCGCCT
GCTGCCAACTGTCGATGGTAAACGCTGCGCTGCCTTCGAGATCTTGTTGCGAACGTCCAGTGTCAGCGAGCTGATCAAGC
GAGGAGAGATTGGTGAGCTGAAAGACATTATGGCCAAATCCAATCACATCGGCATGATGACCTTCGACCAGTCCATCTTC
AATCTGTTCAAGGAAGGCAAGATCACAGAAGAAACCGCCATGCGTAATGCTGATTCTCCAAACAACATGCGCCTGATGAT
CAAGTTTCTTAAAGACGGCCAGGGTATGGCGGATCGAAAAGTGCCGGAGTTTAATACAGGCGGCAAGCCGCCAGCATTTG
AGGTGGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(38-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.742

94.947

0.529
  pilU Acinetobacter baylyi ADP1

51.515

96.543

0.497
  pilU Vibrio cholerae strain A1552

52.436

92.819

0.487
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.214

99.202

0.399
  pilT Acinetobacter baylyi ADP1

40.233

91.223

0.367
  pilT Neisseria meningitidis 8013

39.884

92.021

0.367
  pilT Acinetobacter baumannii strain A118

39.826

91.489

0.364
  pilT Acinetobacter baumannii D1279779

39.826

91.489

0.364
  pilT Acinetobacter nosocomialis M2

39.826

91.489

0.364

Multiple sequence alignment