Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACLEAH_RS25010 Genome accession   NZ_CP177242
Coordinates   5337087..5338304 (+) Length   405 a.a.
NCBI ID   WP_033995372.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SM45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5332087..5343304
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLEAH_RS24985 (ACLEAH_24985) nadC 5332190..5333038 (+) 849 WP_033995373.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACLEAH_RS24995 (ACLEAH_24995) - 5333219..5334532 (-) 1314 WP_231280937.1 O-antigen ligase family protein -
  ACLEAH_RS25000 (ACLEAH_25000) - 5334688..5335152 (-) 465 WP_023092872.1 pilin -
  ACLEAH_RS25005 (ACLEAH_25005) pilB 5335383..5337083 (+) 1701 WP_023092873.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLEAH_RS25010 (ACLEAH_25010) pilC 5337087..5338304 (+) 1218 WP_033995372.1 type II secretion system F family protein Machinery gene
  ACLEAH_RS25015 (ACLEAH_25015) pilD 5338305..5339177 (+) 873 WP_023092875.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ACLEAH_RS25020 (ACLEAH_25020) coaE 5339174..5339785 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  ACLEAH_RS25025 (ACLEAH_25025) yacG 5339782..5339982 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  ACLEAH_RS25030 (ACLEAH_25030) - 5340019..5340228 (-) 210 WP_003094660.1 hypothetical protein -
  ACLEAH_RS25035 (ACLEAH_25035) - 5340334..5341023 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  ACLEAH_RS25040 (ACLEAH_25040) - 5341020..5341490 (-) 471 WP_003094664.1 hypothetical protein -
  ACLEAH_RS25045 (ACLEAH_25045) - 5341487..5341912 (-) 426 WP_003094666.1 GNAT family N-acetyltransferase -
  ACLEAH_RS25050 (ACLEAH_25050) - 5342045..5342674 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  ACLEAH_RS25055 (ACLEAH_25055) - 5342671..5343120 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44086.87 Da        Isoelectric Point: 9.7134

>NTDB_id=1086404 ACLEAH_RS25010 WP_033995372.1 5337087..5338304(+) (pilC) [Pseudomonas aeruginosa strain SM45]
MADKALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPMDIALFTRQMSTMMA
AGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVASGNSLANSLRKKPQYFDSLYCNLVDAGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFEGFGAELPAFTQMVINISNVLQEWWLLVLLMMGGAGFLLN
HAYKRSEKFRDATDRTVLKLPIVGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRDAVDKIKQDVSTGM
QLNFSMRTTNIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1086404 ACLEAH_RS25010 WP_033995372.1 5337087..5338304(+) (pilC) [Pseudomonas aeruginosa strain SM45]
ATGGCGGACAAAGCGTTAAAAACCAGTGTCTTCGTATGGGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCT
GTCCAGCCAGAACCCGACCCTGGTCAAGGCGCAACTGCGCAAGCAGGGCATCACGCCGGTCAAGGTCCGCAAGAAAGGCA
TTTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCTATGGACATCGCACTGTTCACCCGCCAGATGTCCACCATGATGGCC
GCCGGCGTGCCACTGCTGCAGTCGTTCGACATCATCAGCGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGA
GATCAAGCAGGAAGTCGCCAGCGGCAACAGCCTGGCCAACTCGCTGCGCAAGAAGCCGCAGTACTTCGACTCGCTCTACT
GCAACCTGGTGGACGCCGGCGAACAATCCGGCGCCCTGGAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTACCCAATCGCGGTGATCGTCGTCGCCATCATCGTTTCGGCGAT
CCTACTGATCAAGGTGGTGCCGCAGTTCCAGTCGGTGTTCGAGGGCTTCGGCGCCGAACTGCCGGCCTTCACCCAAATGG
TCATCAATATCTCCAACGTGCTCCAGGAATGGTGGCTCCTGGTACTGCTGATGATGGGCGGCGCCGGCTTCCTGCTGAAC
CACGCCTACAAGCGTTCGGAGAAGTTTCGCGACGCCACCGACCGCACGGTACTGAAGCTGCCGATCGTCGGCGCCATCCT
CTACAAATCCGCCGTGGCACGCTACGCGCGGACGCTGTCGACCACCTTCGCCGCCGGCGTGCCATTGGTGGAAGCCCTCG
ACTCGGTTTCCGGCGCCACCGGCAACGTGGTGTTCAGGGACGCGGTGGACAAGATCAAGCAGGACGTTTCCACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCAACATCTTCCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAATCGGG
CGCGCTGGACGACATGCTGGCCAAGGTCGCTGGCTTCTACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCC
TGATGGAACCGATGATCATGGCGGTCCTCGGGGTCCTGGTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAG
TTGGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.765

100

0.788
  pilC Acinetobacter baylyi ADP1

61.029

100

0.615
  pilC Acinetobacter baumannii D1279779

60.396

99.753

0.602
  pilC Legionella pneumophila strain ERS1305867

54.545

97.778

0.533
  pilG Neisseria gonorrhoeae MS11

46.287

99.753

0.462
  pilG Neisseria meningitidis 44/76-A

45.771

99.259

0.454
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Vibrio campbellii strain DS40M4

40.852

98.519

0.402
  pilC Thermus thermophilus HB27

37.905

99.012

0.375

Multiple sequence alignment