Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLEAH_RS25005 Genome accession   NZ_CP177242
Coordinates   5335383..5337083 (+) Length   566 a.a.
NCBI ID   WP_023092873.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SM45     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5330383..5342083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLEAH_RS24985 (ACLEAH_24985) nadC 5332190..5333038 (+) 849 WP_033995373.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACLEAH_RS24995 (ACLEAH_24995) - 5333219..5334532 (-) 1314 WP_231280937.1 O-antigen ligase family protein -
  ACLEAH_RS25000 (ACLEAH_25000) - 5334688..5335152 (-) 465 WP_023092872.1 pilin -
  ACLEAH_RS25005 (ACLEAH_25005) pilB 5335383..5337083 (+) 1701 WP_023092873.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLEAH_RS25010 (ACLEAH_25010) pilC 5337087..5338304 (+) 1218 WP_033995372.1 type II secretion system F family protein Machinery gene
  ACLEAH_RS25015 (ACLEAH_25015) pilD 5338305..5339177 (+) 873 WP_023092875.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ACLEAH_RS25020 (ACLEAH_25020) coaE 5339174..5339785 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  ACLEAH_RS25025 (ACLEAH_25025) yacG 5339782..5339982 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  ACLEAH_RS25030 (ACLEAH_25030) - 5340019..5340228 (-) 210 WP_003094660.1 hypothetical protein -
  ACLEAH_RS25035 (ACLEAH_25035) - 5340334..5341023 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  ACLEAH_RS25040 (ACLEAH_25040) - 5341020..5341490 (-) 471 WP_003094664.1 hypothetical protein -
  ACLEAH_RS25045 (ACLEAH_25045) - 5341487..5341912 (-) 426 WP_003094666.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62343.23 Da        Isoelectric Point: 5.5798

>NTDB_id=1086403 ACLEAH_RS25005 WP_023092873.1 5335383..5337083(+) (pilB) [Pseudomonas aeruginosa strain SM45]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLSVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
DKIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1086403 ACLEAH_RS25005 WP_023092873.1 5335383..5337083(+) (pilB) [Pseudomonas aeruginosa strain SM45]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCATCAGGCGGTCACCGATATCCAATTCAGCACCGGCCTCACCACCGAAGCCCTGCTGGTAGAGGACG
ACAAGCTCAGCGTAGCCATCGAGAAGCTTTACGAGGGCGCCATGGACGCCCTCGGCGACCTCGGCGATATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGATGCCCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAGGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGCATTTCCGCGCGACTC
AAGGTGATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAACAA
GTCGATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAAGAGGATCAGAAGGAACTCTATCTGGCTGCGCTCAAACAGCCACAGGGC
ATGATACTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTACGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCATTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.544

100

0.58
  pilB Acinetobacter baylyi ADP1

56.79

100

0.569
  pilB Legionella pneumophila strain ERS1305867

54.674

100

0.548
  pilB Vibrio cholerae strain A1552

51.264

97.88

0.502
  pilF Neisseria gonorrhoeae MS11

50.177

99.647

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

52.622

94.346

0.496
  pilB Vibrio campbellii strain DS40M4

51.22

94.17

0.482
  pilB/pilB1 Synechocystis sp. PCC 6803

37.763

100

0.412
  pilF Thermus thermophilus HB27

38.664

100

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.727

97.173

0.376

Multiple sequence alignment