Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACK56M_RS27490 Genome accession   NZ_CP177209
Coordinates   5937872..5939140 (-) Length   422 a.a.
NCBI ID   WP_080604909.1    Uniprot ID   -
Organism   Pseudomonas sp. s4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5932872..5944140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK56M_RS27475 (ACK56M_27475) - 5934505..5936139 (-) 1635 WP_100412792.1 SPOR domain-containing protein -
  ACK56M_RS27480 (ACK56M_27480) aroB 5936155..5937252 (-) 1098 WP_015272135.1 3-dehydroquinate synthase -
  ACK56M_RS27485 (ACK56M_27485) aroK 5937349..5937867 (-) 519 WP_015272136.1 shikimate kinase AroK -
  ACK56M_RS27490 (ACK56M_27490) pilQ 5937872..5939140 (-) 1269 WP_080604909.1 type IV pilus secretin PilQ Machinery gene
  ACK56M_RS27495 (ACK56M_27495) - 5939064..5940065 (-) 1002 WP_043936291.1 pilus assembly protein PilP -
  ACK56M_RS27500 (ACK56M_27500) - 5940077..5940613 (-) 537 WP_139657891.1 PilN domain-containing protein -
  ACK56M_RS27505 (ACK56M_27505) pilM 5940610..5941491 (-) 882 WP_054572203.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46021.84 Da        Isoelectric Point: 4.9200

>NTDB_id=1086189 ACK56M_RS27490 WP_080604909.1 5937872..5939140(-) (pilQ) [Pseudomonas sp. s4]
MKRLMKWMLAALLPFPLASAMPYQGEPLSLNFQDVEVRSVLQVLADYAGVNLVASDAVQGSVTLRLEDVPWDQALDLVLR
SKGLARRQEGNVLLVAPAAEFAAQSVDAHVGQALDAQLQPLRRELLPIHHAKAAELAELLLASLADDGILNGRGSLSVDE
RTNTLVVYQPADRLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYVENPRPGKDLFVDLGVERVGGSI
GLGLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSVAFREASLSLEVTPQITPDN
KVIMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVTDGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=1086189 ACK56M_RS27490 WP_080604909.1 5937872..5939140(-) (pilQ) [Pseudomonas sp. s4]
ATGAAAAGATTGATGAAATGGATGCTGGCAGCCCTGCTGCCGTTCCCGCTGGCGTCGGCAATGCCTTATCAGGGTGAACC
ACTGTCGCTGAACTTCCAGGATGTGGAAGTGCGCTCGGTGCTGCAGGTGCTGGCTGACTATGCCGGTGTCAACCTGGTTG
CCAGCGATGCCGTGCAGGGTAGCGTCACCTTGCGCCTTGAGGATGTTCCATGGGACCAGGCGCTGGATCTGGTGCTGCGC
AGCAAGGGCTTGGCCCGGCGACAGGAGGGCAATGTGCTGTTGGTGGCGCCCGCGGCGGAGTTTGCCGCGCAGTCTGTCGA
TGCCCATGTGGGCCAGGCGCTGGATGCACAGTTGCAGCCGTTGCGTCGGGAGCTGTTGCCCATTCATCACGCCAAGGCCG
CCGAGCTGGCAGAGCTGCTGCTGGCGAGCCTGGCGGATGACGGCATCCTCAACGGTCGGGGCAGCCTGAGTGTCGATGAG
CGCACCAATACGCTGGTGGTGTACCAGCCGGCAGACCGCCTTGCCGAACTGAGGCAGCTGGTGGCGCAGCTGGATGTGCC
GGTGCGTCAGGTGGCGATCGAGGCACGTATCGTCGAGGCCAACGTCGATTACGAGAAAAGCCTGGGGGTGCGCTGGGGTG
GGCCGCTGTATGTCGAGAACCCGCGCCCGGGCAAGGATCTGTTCGTCGATCTCGGGGTGGAGCGGGTGGGCGGTAGCATC
GGTCTGGGCTTGTTGCGTGGCGATGTGCTGCTCGACCTGGAACTCAGTGCCATGGAAAAGAGCGGCAACGGCGAAATCAT
CTCGCAGCCCAAGGTGGTCACGGCCGACAAGGAAACGGCCAGGATCCTCAAAGGCACCGAGGTGCCGTACCAGGAAACCA
GCAAGAGCGGCGCCACCTCGGTAGCGTTCCGCGAAGCCTCGTTGTCGCTGGAAGTGACCCCGCAGATCACTCCGGACAAC
AAGGTGATCATGACCGTCAGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAA
GAACGAGGTCAATGCGAAGGTTCGCGTGACGGACGGAGAAACGATCGTGATCGGTGGCGTGTACTCGACTTCGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II and III secretion system protein (Secretin)

(257-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.404

100

0.614
  pilQ Acinetobacter baumannii strain A118

41.921

100

0.455
  pilQ Acinetobacter baumannii D1279779

41.921

100

0.455
  comQ Acinetobacter baylyi ADP1

42.257

100

0.453
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.014

100

0.422
  pilQ Vibrio cholerae strain A1552

41.014

100

0.422
  pilQ Legionella pneumophila strain ERS1305867

42.344

99.052

0.419
  pilQ Legionella pneumophila strain Lp02

42.344

99.052

0.419
  pilQ Vibrio campbellii strain DS40M4

40.741

100

0.417
  pilQ Neisseria gonorrhoeae strain FA1090

36.842

99.052

0.365

Multiple sequence alignment