Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACK8HJ_RS21540 Genome accession   NZ_CP177050
Coordinates   4618171..4619403 (+) Length   410 a.a.
NCBI ID   WP_009286592.1    Uniprot ID   L9UDH1
Organism   Vreelandella titanicae strain RF40     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4613171..4624403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK8HJ_RS21520 (ACK8HJ_21520) - 4614203..4615024 (-) 822 WP_009286596.1 inositol monophosphatase family protein -
  ACK8HJ_RS21525 (ACK8HJ_21525) - 4615119..4615553 (-) 435 WP_009286595.1 anti-virulence regulator CigR family protein -
  ACK8HJ_RS21530 (ACK8HJ_21530) - 4615662..4616177 (-) 516 WP_009286594.1 pilin -
  ACK8HJ_RS21535 (ACK8HJ_21535) pilB 4616419..4618167 (+) 1749 WP_039858366.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACK8HJ_RS21540 (ACK8HJ_21540) pilC 4618171..4619403 (+) 1233 WP_009286592.1 type II secretion system F family protein Machinery gene
  ACK8HJ_RS21545 (ACK8HJ_21545) pilD 4619596..4620441 (+) 846 WP_039858364.1 prepilin peptidase Machinery gene
  ACK8HJ_RS21550 (ACK8HJ_21550) coaE 4620510..4621112 (+) 603 WP_039858363.1 dephospho-CoA kinase -
  ACK8HJ_RS21555 (ACK8HJ_21555) yacG 4621148..4621381 (+) 234 WP_022523595.1 DNA gyrase inhibitor YacG -
  ACK8HJ_RS21560 (ACK8HJ_21560) - 4621421..4622308 (+) 888 WP_009286590.1 DNA replication terminus site-binding protein -
  ACK8HJ_RS21565 (ACK8HJ_21565) - 4622332..4622691 (-) 360 WP_039858361.1 hypothetical protein -
  ACK8HJ_RS21570 (ACK8HJ_21570) - 4623078..4623323 (+) 246 Protein_4236 helix-turn-helix transcriptional regulator -
  ACK8HJ_RS21575 (ACK8HJ_21575) - 4623453..4623896 (+) 444 WP_233974113.1 hypothetical protein -
  ACK8HJ_RS21580 (ACK8HJ_21580) - 4623985..4624359 (-) 375 WP_022523599.1 histone-like nucleoid-structuring protein, MvaT/MvaU family -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 44816.75 Da        Isoelectric Point: 10.5430

>NTDB_id=1085324 ACK8HJ_RS21540 WP_009286592.1 4618171..4619403(+) (pilC) [Vreelandella titanicae strain RF40]
MAKTARRRQTPAIKLARWKWIGKGPQDRALSGEMIGRSKAEVAAKLTKQNIVIRRISKKGNLGGNGRVNPNDVMVFARQM
ATMVRAGIPLLQALQVVAESLKKPAMVALMQQMISDVSSGSSFSDALNRHPKHFDRLFVNLVSAGEQSGALDQMLDRLAT
YKEKVESLKARVKKALWYPTTVMTIGIAVTMLLLIKVVPEFESMFQSFGAELPALTQLTVNLSELAQRYWLVALGAVFAA
MLLLKISIQRSPNVAYRMHALLLRLPVIGDIVHKSAIARFSRTLATTFASGVPLVEGLDTAAGATGNKVYERAVVQTRQD
VATGQQLHFAMRMTNRFPPLAIQMVSIGEEAGSLDAMLNRVADYYEEEVDNKVDALTSLMEPVIIVVLGVLVGGVVVSMY
LPIFDLGAAL

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=1085324 ACK8HJ_RS21540 WP_009286592.1 4618171..4619403(+) (pilC) [Vreelandella titanicae strain RF40]
ATGGCTAAAACGGCACGTCGACGCCAAACGCCCGCCATCAAGCTAGCGCGCTGGAAATGGATCGGTAAAGGCCCCCAGGA
TAGAGCGCTAAGCGGTGAGATGATTGGCCGCAGTAAAGCCGAAGTAGCCGCTAAACTCACCAAGCAAAATATCGTTATCC
GCCGGATTAGTAAAAAAGGCAACCTAGGCGGCAACGGCCGAGTTAACCCCAACGACGTTATGGTCTTTGCGCGCCAGATG
GCCACAATGGTTCGCGCCGGTATCCCCTTGCTACAGGCGCTTCAAGTGGTGGCAGAGAGCCTCAAAAAACCTGCTATGGT
TGCCCTAATGCAACAGATGATAAGCGATGTATCATCGGGCTCCAGCTTCTCTGATGCATTGAACCGCCACCCCAAACACT
TTGATCGCCTGTTTGTTAACCTAGTCAGCGCCGGCGAGCAGTCAGGTGCGCTGGATCAAATGCTTGATCGTCTTGCGACC
TATAAGGAGAAGGTTGAGTCGCTGAAAGCGCGAGTAAAAAAAGCGCTTTGGTACCCCACCACCGTTATGACGATTGGCAT
TGCTGTGACCATGTTGCTGTTGATTAAAGTGGTGCCTGAATTTGAAAGCATGTTCCAGAGCTTTGGCGCTGAACTTCCAG
CACTGACCCAGTTGACCGTGAATTTATCCGAGCTTGCCCAGCGTTATTGGCTAGTGGCACTCGGCGCAGTGTTTGCCGCC
ATGCTCCTGCTCAAAATAAGCATTCAGCGCTCGCCCAATGTCGCCTACCGCATGCACGCACTGCTGCTTCGGTTACCGGT
CATTGGCGACATCGTGCATAAATCCGCGATCGCGCGCTTTTCTCGCACGTTAGCGACGACCTTTGCCTCCGGTGTACCGC
TCGTAGAAGGCTTAGATACCGCCGCCGGTGCCACTGGCAATAAAGTGTACGAACGCGCGGTGGTACAAACGCGCCAGGAC
GTAGCCACCGGGCAACAGCTACACTTCGCCATGCGGATGACCAACCGCTTTCCCCCCTTGGCAATCCAAATGGTCAGTAT
CGGTGAAGAAGCGGGGTCGCTGGATGCCATGCTTAATCGTGTTGCCGATTACTACGAAGAAGAAGTCGACAATAAGGTCG
ATGCTCTTACCTCGCTCATGGAGCCGGTTATTATTGTAGTACTGGGCGTGCTCGTTGGCGGTGTGGTTGTCTCTATGTAC
TTACCCATTTTCGACTTGGGCGCAGCCCTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(76-199)

Type II secretion system (T2SS), protein F (T2SSF)

(280-402)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L9UDH1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Acinetobacter baylyi ADP1

51.958

93.415

0.485
  pilC Acinetobacter baumannii D1279779

51.697

93.415

0.483
  pilC Pseudomonas stutzeri DSM 10701

48.622

97.317

0.473
  pilC Legionella pneumophila strain ERS1305867

47.044

99.024

0.466
  pilC Vibrio cholerae strain A1552

43.735

99.268

0.434
  pilC Vibrio campbellii strain DS40M4

42.998

99.268

0.427
  pilG Neisseria gonorrhoeae MS11

43.216

97.073

0.42
  pilG Neisseria meningitidis 44/76-A

43.979

93.171

0.41

Multiple sequence alignment