Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACK8HJ_RS21535 Genome accession   NZ_CP177050
Coordinates   4616419..4618167 (+) Length   582 a.a.
NCBI ID   WP_039858366.1    Uniprot ID   -
Organism   Vreelandella titanicae strain RF40     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4611419..4623167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK8HJ_RS21500 (ACK8HJ_21500) - 4611564..4611767 (+) 204 WP_039858369.1 excisionase -
  ACK8HJ_RS21505 (ACK8HJ_21505) - 4611769..4612893 (+) 1125 WP_411572259.1 tyrosine-type recombinase/integrase -
  ACK8HJ_RS21510 (ACK8HJ_21510) - 4613043..4613855 (+) 813 WP_230806216.1 hypothetical protein -
  ACK8HJ_RS21520 (ACK8HJ_21520) - 4614203..4615024 (-) 822 WP_009286596.1 inositol monophosphatase family protein -
  ACK8HJ_RS21525 (ACK8HJ_21525) - 4615119..4615553 (-) 435 WP_009286595.1 anti-virulence regulator CigR family protein -
  ACK8HJ_RS21530 (ACK8HJ_21530) - 4615662..4616177 (-) 516 WP_009286594.1 pilin -
  ACK8HJ_RS21535 (ACK8HJ_21535) pilB 4616419..4618167 (+) 1749 WP_039858366.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACK8HJ_RS21540 (ACK8HJ_21540) pilC 4618171..4619403 (+) 1233 WP_009286592.1 type II secretion system F family protein Machinery gene
  ACK8HJ_RS21545 (ACK8HJ_21545) pilD 4619596..4620441 (+) 846 WP_039858364.1 prepilin peptidase Machinery gene
  ACK8HJ_RS21550 (ACK8HJ_21550) coaE 4620510..4621112 (+) 603 WP_039858363.1 dephospho-CoA kinase -
  ACK8HJ_RS21555 (ACK8HJ_21555) yacG 4621148..4621381 (+) 234 WP_022523595.1 DNA gyrase inhibitor YacG -
  ACK8HJ_RS21560 (ACK8HJ_21560) - 4621421..4622308 (+) 888 WP_009286590.1 DNA replication terminus site-binding protein -
  ACK8HJ_RS21565 (ACK8HJ_21565) - 4622332..4622691 (-) 360 WP_039858361.1 hypothetical protein -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 64006.67 Da        Isoelectric Point: 6.9693

>NTDB_id=1085323 ACK8HJ_RS21535 WP_039858366.1 4616419..4618167(+) (pilB) [Vreelandella titanicae strain RF40]
MSQPSLESTLSHASAHGDLRGIAQRLVKHGLLTNAQAATAETTASDLEISLLQHVIESGLVDPNKATLAAGWEYGLPVIN
LEAVRLTSLPPAADYPVKVLQSLNVLPLARHGHRLTVAVPYPATLTRLDELQFATGLSIEGILAPVDQITVVLNSYLAHN
ERGMLDELDDVVSSLSIMQNNGGNDVPLEESSQNSEDAPIVKFVNKILLDAIRRGASDIHFEPYEALYRVRFRVDGMLIE
VARPPFAMRNRIAARLKIMARLDISERRLPQDGSIKLKLSRTRSLDFRVNSLPTVYGEKLVLRILDPTAMQLGIEQLGFT
DEQRRHYEYALKQPQGMILVTGPTGSGKTVSLYTGINILNKVERNICTAEDPVEIKVAGINQVNVLPKIGLDFASALRAF
LRQDPDVVMVGEIRDLETAEIAVKAAQTGHLVLSTVHTNSAAETLTRLANMGVSSFNIASAVSLIVAQRLARKLCTHCKE
PIDIPHEALRKEGFSSEEIHQATIYKPLGCKHCTHGYKGRIGIYEVVPITEAMRQLIMRDANALDIDQQVRKEGYPSLHR
SGLLKVMQGITSLEEVNRVSKE

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=1085323 ACK8HJ_RS21535 WP_039858366.1 4616419..4618167(+) (pilB) [Vreelandella titanicae strain RF40]
ATGAGCCAGCCCTCCTTAGAGTCGACTTTAAGCCACGCCTCCGCACATGGCGACCTGCGCGGCATTGCCCAGCGCTTGGT
CAAGCACGGCTTGCTGACCAACGCCCAGGCCGCAACAGCGGAAACAACCGCGTCGGACTTGGAAATATCGTTACTTCAGC
ATGTGATCGAAAGCGGCCTGGTCGACCCTAACAAAGCCACACTGGCGGCTGGCTGGGAGTATGGGCTTCCAGTCATTAAC
CTGGAGGCGGTGCGGCTTACCTCACTGCCCCCCGCTGCAGACTATCCTGTCAAAGTACTGCAGAGCCTTAACGTTTTGCC
ATTAGCGCGCCACGGTCATCGCCTCACGGTGGCGGTGCCCTACCCCGCTACATTGACGCGACTGGATGAATTGCAGTTTG
CAACAGGCCTGAGCATTGAAGGTATCCTCGCGCCCGTTGATCAAATCACTGTCGTTCTCAATAGCTACCTTGCTCACAAC
GAGCGCGGCATGCTGGATGAGTTGGACGATGTGGTCAGCTCGCTGAGCATTATGCAGAACAACGGCGGTAACGATGTACC
GCTGGAAGAGTCTTCCCAAAATAGCGAAGATGCACCCATCGTTAAATTTGTGAACAAAATTCTGCTAGATGCTATCCGCC
GAGGCGCCTCCGATATCCATTTCGAACCTTACGAAGCGCTTTACCGAGTACGCTTTCGGGTTGATGGAATGCTCATAGAA
GTGGCACGGCCACCATTCGCCATGCGCAATCGTATCGCGGCCCGGCTAAAAATCATGGCCCGCCTTGATATTTCCGAGCG
CCGCCTGCCTCAGGATGGCTCGATAAAACTCAAGCTTTCGCGCACCCGCTCCCTTGATTTCCGGGTTAATTCGCTACCTA
CTGTCTATGGTGAAAAGTTGGTGCTGCGAATATTAGACCCTACCGCAATGCAGCTGGGTATTGAGCAACTTGGATTTACC
GACGAACAACGGCGCCATTATGAGTACGCCTTAAAACAACCCCAAGGCATGATTCTAGTAACCGGCCCTACCGGTAGTGG
TAAAACCGTTTCGCTGTATACCGGCATTAATATTCTTAATAAAGTGGAACGCAATATTTGCACCGCCGAAGACCCGGTTG
AAATCAAAGTCGCGGGTATCAATCAGGTGAACGTATTGCCTAAGATCGGCCTCGATTTTGCCAGTGCGTTAAGGGCGTTT
CTGCGCCAAGACCCTGATGTGGTCATGGTGGGCGAGATTCGTGACCTTGAAACAGCCGAAATTGCCGTCAAAGCGGCTCA
GACAGGCCACCTGGTACTTTCCACGGTGCATACCAACTCGGCAGCTGAGACACTGACTCGACTGGCTAATATGGGAGTCT
CTTCGTTTAATATCGCCAGCGCCGTTAGCTTGATTGTTGCCCAACGGCTGGCTCGTAAACTATGCACACACTGCAAAGAG
CCCATTGACATTCCCCACGAGGCGCTGCGCAAAGAAGGCTTTAGTAGCGAAGAAATCCATCAGGCAACGATCTACAAACC
GTTAGGTTGCAAGCACTGTACTCACGGTTATAAAGGTCGAATTGGCATTTATGAGGTAGTGCCGATCACCGAAGCGATGC
GTCAACTAATTATGCGTGATGCCAACGCCTTAGATATTGATCAACAGGTGCGCAAGGAGGGCTATCCAAGCCTTCACCGC
AGTGGGTTACTGAAAGTCATGCAGGGTATTACCAGCCTGGAAGAGGTTAACCGAGTGAGCAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-160)

Type II/IV secretion system protein (T2SSE)

(206-473)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50.707

97.251

0.493
  pilB Acinetobacter baumannii D1279779

50.796

97.079

0.493
  pilB Acinetobacter baylyi ADP1

49.912

97.766

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

47.945

100

0.481
  pilB Vibrio campbellii strain DS40M4

48.325

97.423

0.471
  pilB Vibrio cholerae strain A1552

48.022

95.533

0.459
  pilF Neisseria gonorrhoeae MS11

49.688

82.646

0.411

Multiple sequence alignment