Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACLH0J_RS29765 Genome accession   NZ_CP176771
Coordinates   6538679..6539887 (+) Length   402 a.a.
NCBI ID   WP_411390241.1    Uniprot ID   -
Organism   Pseudomonas sp. MPB23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6533679..6544887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLH0J_RS29740 (ACLH0J_29740) nadC 6535165..6536013 (+) 849 WP_411390238.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACLH0J_RS29745 (ACLH0J_29745) - 6536062..6536367 (-) 306 WP_411390239.1 DUF6388 family protein -
  ACLH0J_RS29755 (ACLH0J_29755) - 6536625..6538025 (-) 1401 WP_411390240.1 O-antigen ligase family protein -
  ACLH0J_RS29760 (ACLH0J_29760) - 6538062..6538475 (-) 414 WP_411392912.1 pilin -
  ACLH0J_RS29765 (ACLH0J_29765) pilC 6538679..6539887 (+) 1209 WP_411390241.1 type II secretion system F family protein Machinery gene
  ACLH0J_RS29770 (ACLH0J_29770) pilD 6539884..6540750 (+) 867 WP_411390242.1 prepilin peptidase Machinery gene
  ACLH0J_RS29775 (ACLH0J_29775) coaE 6540747..6541370 (+) 624 WP_411390243.1 dephospho-CoA kinase -
  ACLH0J_RS29780 (ACLH0J_29780) yacG 6541367..6541567 (+) 201 WP_003188437.1 DNA gyrase inhibitor YacG -
  ACLH0J_RS29785 (ACLH0J_29785) - 6541564..6541779 (-) 216 WP_003171680.1 hypothetical protein -
  ACLH0J_RS29790 (ACLH0J_29790) - 6541855..6542544 (-) 690 WP_411390244.1 energy-coupling factor ABC transporter permease -
  ACLH0J_RS29795 (ACLH0J_29795) - 6542541..6543008 (-) 468 WP_411390245.1 FAD/FMN-containing dehydrogenase -
  ACLH0J_RS29800 (ACLH0J_29800) - 6543108..6543737 (+) 630 WP_010565579.1 DUF1780 domain-containing protein -
  ACLH0J_RS29805 (ACLH0J_29805) - 6543791..6543964 (+) 174 WP_016976295.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43347.61 Da        Isoelectric Point: 10.0466

>NTDB_id=1082870 ACLH0J_RS29765 WP_411390241.1 6538679..6539887(+) (pilC) [Pseudomonas sp. MPB23]
MNNTSTLYAWEGINRKGRRVCGHTTGHNPAMVKAQLRRQGISPGRVHKKSALLPSLAPRIKPADITLLTRQLATLLKAGI
ALLQAFDIISEGVDNRSVRELVQGLKREIAAGTSLTDALRQQPRYFDDLYCNLVAAGEQAGTLETLLERVAIHREKSAQL
RARIKKAMTYPVAVLLIASLVTGVLLIHVVPQFQTLFAGVGGNLPGFTLQVIALSEFMQQTWWVVALGLAAGAVGLRHAY
RASLDFRHWVDAGLLKVPLAGKLLQKSAVARYARTLSTTFAAGVPLVQALDCVAGAAGSGPFKQAIEHMRHDVSTGMQLN
QSMAASSLFPGMAIQMTAIGEESGTLDRMLEKVASHYEADVDNQVDNLTHLMEPLMMVVLGGIVGALVIAMYLPVFQVGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=1082870 ACLH0J_RS29765 WP_411390241.1 6538679..6539887(+) (pilC) [Pseudomonas sp. MPB23]
ATGAACAACACATCGACGCTTTACGCCTGGGAAGGCATCAACCGCAAAGGACGCAGGGTGTGCGGGCACACCACCGGGCA
CAACCCGGCCATGGTCAAGGCGCAACTGCGTCGGCAAGGCATCAGCCCGGGCCGTGTACACAAGAAATCCGCCTTGCTGC
CAAGCCTGGCCCCGCGGATAAAGCCGGCGGACATTACCCTGCTCACTCGCCAACTGGCCACCCTGCTGAAAGCAGGGATC
GCGCTGCTGCAAGCCTTCGACATCATCAGCGAAGGCGTCGACAACCGATCGGTGCGTGAACTGGTGCAGGGGTTGAAGCG
AGAGATCGCCGCCGGCACCAGCCTGACCGATGCATTGCGCCAACAACCGCGTTATTTCGACGACCTGTATTGCAACCTGG
TGGCCGCCGGCGAACAAGCGGGGACCCTTGAGACCCTGCTGGAGCGCGTCGCGATCCACCGGGAAAAGAGCGCACAGCTT
AGAGCCAGAATCAAAAAAGCCATGACCTACCCGGTCGCGGTCCTGCTGATCGCGAGTCTCGTCACGGGCGTTCTGTTGAT
TCACGTGGTGCCGCAATTCCAGACACTGTTCGCTGGGGTCGGCGGCAATCTGCCCGGGTTCACCTTGCAGGTCATCGCCC
TGTCCGAGTTCATGCAGCAAACCTGGTGGGTCGTGGCACTGGGCCTGGCTGCGGGGGCCGTGGGCTTGCGTCATGCCTAT
CGCGCGTCCCTTGATTTTCGTCACTGGGTGGACGCCGGTTTGTTGAAAGTCCCCCTGGCAGGCAAACTGCTGCAAAAATC
GGCCGTGGCCCGCTACGCCCGCACCCTCTCCACCACCTTTGCCGCCGGTGTTCCGCTGGTACAGGCACTGGATTGCGTGG
CCGGCGCGGCAGGCTCAGGGCCGTTCAAACAGGCTATCGAGCATATGCGACACGATGTATCCACGGGCATGCAGTTGAAT
CAATCCATGGCCGCCAGCAGCCTGTTTCCCGGCATGGCAATCCAGATGACAGCGATCGGCGAAGAGTCCGGCACGCTGGA
CCGCATGCTGGAAAAAGTCGCCAGCCATTACGAGGCCGATGTGGATAACCAGGTCGATAACCTCACGCATTTGATGGAGC
CGCTGATGATGGTCGTGCTGGGCGGCATTGTCGGGGCACTGGTCATCGCGATGTACTTGCCGGTGTTCCAAGTGGGGAGC
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(69-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.838

98.507

0.58
  pilC Acinetobacter baylyi ADP1

48.99

98.507

0.483
  pilC Acinetobacter baumannii D1279779

48.608

98.259

0.478
  pilC Legionella pneumophila strain ERS1305867

46.835

98.259

0.46
  pilC Vibrio cholerae strain A1552

40.295

100

0.408
  pilG Neisseria gonorrhoeae MS11

40.42

94.776

0.383
  pilC Vibrio campbellii strain DS40M4

38.579

98.01

0.378
  pilG Neisseria meningitidis 44/76-A

39.633

94.776

0.376
  pilC Thermus thermophilus HB27

36.409

99.751

0.363

Multiple sequence alignment