Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACK2V6_RS13770 Genome accession   NZ_CP176657
Coordinates   2973640..2975382 (-) Length   580 a.a.
NCBI ID   WP_024699327.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain GTVSS-032     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2968640..2980382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2V6_RS13750 (ACK2V6_13750) - 2969322..2970161 (-) 840 WP_005486862.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  ACK2V6_RS13755 (ACK2V6_13755) - 2970231..2971748 (-) 1518 WP_005480444.1 SPOR domain-containing protein -
  ACK2V6_RS13760 (ACK2V6_13760) aroB 2971801..2972901 (-) 1101 WP_005480443.1 3-dehydroquinate synthase -
  ACK2V6_RS13765 (ACK2V6_13765) aroK 2972933..2973451 (-) 519 WP_005381319.1 shikimate kinase AroK -
  ACK2V6_RS13770 (ACK2V6_13770) pilQ 2973640..2975382 (-) 1743 WP_024699327.1 type IV pilus secretin PilQ Machinery gene
  ACK2V6_RS13775 (ACK2V6_13775) pilP 2975414..2975929 (-) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  ACK2V6_RS13780 (ACK2V6_13780) pilO 2975919..2976512 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACK2V6_RS13785 (ACK2V6_13785) pilN 2976505..2977083 (-) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  ACK2V6_RS13790 (ACK2V6_13790) pilM 2977067..2978083 (-) 1017 WP_005480475.1 type IV pilus biogenesis protein PilM Machinery gene

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 63579.71 Da        Isoelectric Point: 4.4560

>NTDB_id=1082115 ACK2V6_RS13770 WP_024699327.1 2973640..2975382(-) (pilQ) [Vibrio parahaemolyticus strain GTVSS-032]
MKMRQGLTRTPRMLTGLFSLWLFLLLAPLAAAEDVQSNALKSIDFRTNKSKDAVIVVELASPAAIVDVKRVQEGLSIDLM
NTSVKDEQLYLLDVKDFATVVESVEVFRDTSTTRLVAYIDEEYTHDYRLTGRYLEITVSKLKPNEKVPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIAVIREIIESLDIPVK
QVQIEARIVTISEGNMDELGIRWGFTSINGNNTVGGSIENNLATIGLYEGGGDGEGEEGGVGIDDFLNVNLAATNPNAAS
IAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQIT
PDNRLVLDLSVTQDRPGKVVKTGTGEAMAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRRS
YENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=1082115 ACK2V6_RS13770 WP_024699327.1 2973640..2975382(-) (pilQ) [Vibrio parahaemolyticus strain GTVSS-032]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATGCTTACTGGGCTCTTCTCCTTATGGTTGTTTTTACTTCTAGC
CCCTTTAGCAGCAGCAGAAGATGTACAGTCTAATGCGCTCAAGAGCATCGACTTTCGCACTAACAAAAGCAAAGATGCCG
TGATTGTGGTGGAGCTTGCATCCCCGGCGGCTATTGTTGATGTTAAACGTGTTCAAGAAGGATTAAGCATTGATTTAATG
AACACGTCGGTAAAAGACGAGCAGCTTTACCTTCTCGACGTGAAAGATTTCGCGACGGTGGTGGAAAGTGTGGAAGTGTT
CCGCGATACCTCCACCACACGATTAGTTGCGTATATCGATGAAGAATACACGCATGACTATCGTTTGACGGGGCGTTATC
TTGAAATCACTGTCAGCAAGCTTAAACCAAATGAAAAAGTGCCAGACAAGAGCATCTTAGAAAAAGAAGGCAAGCTGATC
TCGATTAACTTCCAAGACATTCCTGTGCGTAACGTTTTGCAGCTGATTGCAGATTACAACCAGTTTAACTTGGTGGTTTC
GGACTCTGTCGAAGGCAACCTTACATTGCGTCTTGATGGTGTGCCGTGGCAGCAAGTGCTCGACATCATCTTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTGGTCGCCCCGACCGATGAGCTGGATTTGCGCGAGAAGCAGCAG
TTAGAAAAACAAAAACTGACGGAAGAAATGGGTGAACTTTCATCAGAAATCATCAAAGTCAATTTTGCTAAAGCTTCGGA
TATTGCAGAGATGATCAATGGTGAAGGCAACATTAGCATGTTGTCAGAGCGTGGCAGTATGACCATTGATGAACGAACCA
ATTCGCTATTAATCAGAGAATTGCCAGAGAACATCGCCGTTATTCGCGAAATCATTGAATCGCTCGATATCCCAGTCAAA
CAAGTACAAATTGAAGCCCGAATTGTCACCATCAGTGAAGGTAACATGGACGAGTTAGGTATTCGTTGGGGCTTCACATC
TATTAATGGTAATAACACCGTTGGTGGCTCTATTGAGAACAACCTAGCGACAATCGGTTTGTACGAAGGTGGTGGTGATG
GCGAAGGTGAAGAGGGTGGTGTTGGTATCGATGATTTCCTTAATGTGAATTTAGCAGCGACCAACCCGAATGCGGCGAGT
ATTGCGTTCCAAGTGGCAAAGTTAGGTTCTGATACATTATTGGACTTAGAGCTGTCTGCATTGCAGCAAGAATCAAAAGC
AGAAATCATCTCGAGTCCACGATTGATCACGACCAATAAGAAGCCCGCGTACATTGAACAAGGTACCGAAATTCCTTATC
TAGAATCTTCATCGAGTGGTGCAACGTCAGTGACCTTTAAAAAAGCGGTGTTGAGCTTGAAGGTGACACCGCAGATCACA
CCTGACAATCGTTTAGTGTTGGATCTGAGTGTGACCCAAGACCGACCTGGTAAGGTAGTGAAAACGGGCACAGGAGAGGC
AATGGCAATTGATACGCAGCGCATTGGTACCCAAGTTCTGGTTAATAATGGTGAGACGGTTGTGCTCGGTGGTATTTTTC
AGCACAGCATTACTAACTCAGTGGATAAAGTGCCATTGTTGGGCGATCTTCCGCTATTAGGCGCACTTTTCCGCCGAAGT
TACGAAAACGTTGGTAAAAGTGAGTTGCTAATTTTCGTTACGCCTAAGGTCGTTATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(420-578)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

92.241

100

0.922
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

76.855

97.586

0.75
  pilQ Vibrio cholerae strain A1552

76.855

97.586

0.75
  pilQ Legionella pneumophila strain Lp02

41.166

94.655

0.39
  pilQ Legionella pneumophila strain ERS1305867

41.166

94.655

0.39
  pilQ Pseudomonas aeruginosa PAK

39.336

98.621

0.388
  comQ Acinetobacter baylyi ADP1

38.382

100

0.384
  pilQ Acinetobacter baumannii D1279779

37.037

100

0.379
  pilQ Acinetobacter baumannii strain A118

37.037

100

0.379

Multiple sequence alignment