Detailed information NTG1082119

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACK2V6_RS13790
• Genome accession: NZ_CP176657
• Coordinates: 2977067..2978083 (-)
• Length: 338 a.a.
• NCBI ID: WP_005480475.1
• Uniprot ID: A0A0L7V0I6
• Organism: Vibrio parahaemolyticus strain GTVSS-032
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2972067..2983083

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACK2V6_RS13765 (ACK2V6_13765)	aroK	2972933..2973451 (-)	519	WP_005381319.1	shikimate kinase AroK	-
ACK2V6_RS13770 (ACK2V6_13770)	pilQ	2973640..2975382 (-)	1743	WP_024699327.1	type IV pilus secretin PilQ	Machinery gene
ACK2V6_RS13775 (ACK2V6_13775)	pilP	2975414..2975929 (-)	516	WP_005480477.1	pilus assembly protein PilP	Machinery gene
ACK2V6_RS13780 (ACK2V6_13780)	pilO	2975919..2976512 (-)	594	WP_005480482.1	type 4a pilus biogenesis protein PilO	Machinery gene
ACK2V6_RS13785 (ACK2V6_13785)	pilN	2976505..2977083 (-)	579	WP_005480484.1	PilN domain-containing protein	Machinery gene
ACK2V6_RS13790 (ACK2V6_13790)	pilM	2977067..2978083 (-)	1017	WP_005480475.1	type IV pilus biogenesis protein PilM	Machinery gene
ACK2V6_RS13795 (ACK2V6_13795)	-	2978226..2980796 (+)	2571	WP_005480476.1	penicillin-binding protein 1A	-
ACK2V6_RS13800 (ACK2V6_13800)	oxyR	2980852..2981760 (-)	909	WP_005465160.1	DNA-binding transcriptional regulator OxyR	-
ACK2V6_RS13805 (ACK2V6_13805)	-	2981931..2982659 (+)	729	WP_005465158.1	redoxin family protein	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37776.31 Da
• Isoelectric Point: 6.8323

>NTDB_id=1082119 ACK2V6_RS13790 WP_005480475.1 2977067..2978083(-) (pilM) [Vibrio parahaemolyticus strain GTVSS-032]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRALSH

Nucleotide

• Length: 1017 bp

>NTDB_id=1082119 ACK2V6_RS13790 WP_005480475.1 2977067..2978083(-) (pilM) [Vibrio parahaemolyticus strain GTVSS-032]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAATTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAAGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATCTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-326)

3D structure

• AlphaFold DB: A0A0L7V0I6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio campbellii strain DS40M4	84.911	100	0.849
pilM	Vibrio cholerae strain A1552	48.368	99.704	0.482