Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACKZH3_RS22195 Genome accession   NZ_CP176572
Coordinates   4850977..4852302 (-) Length   441 a.a.
NCBI ID   WP_095004334.1    Uniprot ID   -
Organism   Pseudomonas fragi strain LKYC.Zb1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4845977..4857302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKZH3_RS22180 (ACKZH3_22180) - 4847615..4849186 (-) 1572 WP_016783192.1 AAA family ATPase -
  ACKZH3_RS22185 (ACKZH3_22185) aroB 4849197..4850297 (-) 1101 WP_088378417.1 3-dehydroquinate synthase -
  ACKZH3_RS22190 (ACKZH3_22190) aroK 4850454..4850972 (-) 519 WP_029611693.1 shikimate kinase AroK -
  ACKZH3_RS22195 (ACKZH3_22195) pilQ 4850977..4852302 (-) 1326 WP_095004334.1 type IV pilus secretin PilQ Machinery gene
  ACKZH3_RS22200 (ACKZH3_22200) - 4852314..4852841 (-) 528 WP_409498065.1 pilus assembly protein PilP -
  ACKZH3_RS22205 (ACKZH3_22205) - 4852838..4853458 (-) 621 WP_409498066.1 type 4a pilus biogenesis protein PilO -
  ACKZH3_RS22210 (ACKZH3_22210) - 4853458..4854024 (-) 567 WP_088378421.1 PilN domain-containing protein -
  ACKZH3_RS22215 (ACKZH3_22215) pilM 4854024..4854950 (-) 927 WP_409498067.1 type IV pilus assembly protein PilM -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47383.39 Da        Isoelectric Point: 5.0583

>NTDB_id=1081034 ACKZH3_RS22195 WP_095004334.1 4850977..4852302(-) (pilQ) [Pseudomonas fragi strain LKYC.Zb1]
MKRISPVCVLALWSALNSQATLAAVQAAPSSVASYVGEPMSLNVQNLEVRTVLQSLADFNGFNLMVSDTVKGSITLSLQD
VPWDQALALVLGSKGLGKRLEGNVLWVAPLDEIAARERPEPAPLRRELVQVNYARAADIVMLFQSVTGGQAGLEGQGTIT
VDERTNNIIAFESSERLDELRRIVAQLDVPVRQVMIEAWIVEAGVDFEKSLGVRWGGASSNSGLWSASGITQPLESGAAP
GNASAPFVDLGVSRNSSGLGIAFVTNNVLLDLELTAMEKTGNGEIISQPKVVTADKETAKILKGTQIPYQQASKTGATSV
SFKEASLSLEVTPQITPDNSVIMAVVVTKDEPDYMSRLQDVPPIKKNEVRAKVLVKDGETIVIGGVFSKTQSKVVDKVPF
LGDLPYVGRLFHRDVVLEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=1081034 ACKZH3_RS22195 WP_095004334.1 4850977..4852302(-) (pilQ) [Pseudomonas fragi strain LKYC.Zb1]
ATGAAAAGGATTTCACCGGTCTGCGTACTGGCGCTATGGAGTGCGCTGAATTCGCAGGCAACACTGGCGGCCGTGCAGGC
CGCGCCTTCATCCGTCGCGAGCTATGTGGGCGAACCCATGTCCCTGAACGTGCAGAACCTTGAAGTACGCACGGTGCTGC
AATCGCTGGCGGATTTTAACGGGTTCAACCTGATGGTCAGCGACACCGTGAAAGGCAGTATCACCCTCAGTTTGCAGGAC
GTGCCCTGGGATCAGGCGCTGGCTCTGGTGCTTGGCAGCAAGGGCCTGGGCAAGCGCCTTGAGGGTAATGTGCTGTGGGT
CGCGCCCCTGGACGAGATCGCCGCCCGCGAGCGGCCAGAGCCTGCGCCTTTGCGTCGGGAGCTGGTGCAGGTCAATTACG
CCAGGGCGGCAGATATTGTCATGCTGTTTCAGTCAGTGACCGGCGGTCAGGCCGGTCTCGAGGGGCAGGGCACGATCACG
GTAGATGAACGCACCAATAACATCATTGCCTTTGAATCCAGTGAACGGCTGGATGAGCTGCGGCGCATCGTGGCCCAACT
GGACGTGCCGGTGCGCCAGGTGATGATTGAGGCCTGGATTGTCGAGGCGGGAGTGGACTTCGAAAAAAGCCTGGGTGTTC
GCTGGGGTGGGGCGTCAAGCAACAGCGGCCTGTGGAGTGCCAGCGGCATCACCCAGCCACTGGAGTCAGGGGCTGCGCCC
GGCAACGCCAGCGCGCCTTTTGTCGACCTGGGGGTTTCGCGCAACTCATCGGGGTTGGGCATCGCCTTTGTGACCAACAA
TGTGCTGCTGGACCTGGAGCTGACGGCCATGGAAAAAACCGGTAATGGCGAAATTATCTCGCAGCCCAAAGTGGTTACCG
CAGACAAGGAAACCGCCAAAATCCTCAAAGGCACGCAAATTCCCTATCAACAGGCCAGCAAAACCGGCGCTACATCGGTT
TCGTTCAAGGAGGCCTCGCTGTCGCTGGAGGTGACGCCGCAAATCACCCCGGACAACAGCGTCATCATGGCCGTCGTGGT
GACCAAGGATGAGCCTGATTACATGAGCCGGTTGCAGGATGTACCGCCCATCAAGAAGAACGAGGTCAGGGCCAAGGTGT
TGGTCAAGGATGGTGAAACCATCGTGATTGGGGGGGTGTTCTCAAAAACTCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATCTGCCGTATGTTGGCCGACTTTTCCATAGAGACGTTGTCCTGGAAAAAAAATCCGAACTGCTGGTGTTCCT
GACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(276-432)

Bacterial type II/III secretion system short domain (Secretin_N)

(128-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

65.068

99.32

0.646
  comQ Acinetobacter baylyi ADP1

42.316

100

0.431
  pilQ Acinetobacter baumannii strain A118

39.278

100

0.419
  pilQ Acinetobacter baumannii D1279779

39.278

100

0.419
  pilQ Vibrio campbellii strain DS40M4

40.179

100

0.408
  pilQ Legionella pneumophila strain Lp02

41.805

95.465

0.399
  pilQ Legionella pneumophila strain ERS1305867

41.568

95.465

0.397
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.906

96.599

0.385
  pilQ Vibrio cholerae strain A1552

39.906

96.599

0.385

Multiple sequence alignment