Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACKZH3_RS04085 Genome accession   NZ_CP176572
Coordinates   852535..853752 (-) Length   405 a.a.
NCBI ID   WP_088378957.1    Uniprot ID   -
Organism   Pseudomonas fragi strain LKYC.Zb1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 847535..858752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKZH3_RS04050 (ACKZH3_04050) - 848566..848739 (-) 174 WP_088378952.1 DUF3094 family protein -
  ACKZH3_RS04055 (ACKZH3_04055) - 848794..849321 (-) 528 WP_409498337.1 MOSC domain-containing protein -
  ACKZH3_RS04060 (ACKZH3_04060) - 849318..849947 (-) 630 WP_088378953.1 DUF1780 domain-containing protein -
  ACKZH3_RS04065 (ACKZH3_04065) - 850057..850743 (+) 687 WP_088378954.1 energy-coupling factor ABC transporter permease -
  ACKZH3_RS04070 (ACKZH3_04070) yacG 850847..851050 (-) 204 WP_016779444.1 DNA gyrase inhibitor YacG -
  ACKZH3_RS04075 (ACKZH3_04075) coaE 851047..851670 (-) 624 WP_088378955.1 dephospho-CoA kinase -
  ACKZH3_RS04080 (ACKZH3_04080) pilD 851672..852532 (-) 861 WP_409498338.1 prepilin peptidase Machinery gene
  ACKZH3_RS04085 (ACKZH3_04085) pilC 852535..853752 (-) 1218 WP_088378957.1 type II secretion system F family protein Machinery gene
  ACKZH3_RS04090 (ACKZH3_04090) - 853964..854371 (+) 408 WP_088378958.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACKZH3_RS04100 (ACKZH3_04100) - 854664..856154 (+) 1491 WP_409498339.1 AlkA N-terminal domain-containing protein -
  ACKZH3_RS04105 (ACKZH3_04105) - 856157..856648 (+) 492 WP_409498340.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  ACKZH3_RS04110 (ACKZH3_04110) nadC 857481..858329 (-) 849 WP_095005077.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43944.52 Da        Isoelectric Point: 9.9390

>NTDB_id=1081022 ACKZH3_RS04085 WP_088378957.1 852535..853752(-) (pilC) [Pseudomonas fragi strain LKYC.Zb1]
MATQAVKNTLYRWQGTDGNGVKTSGQISADSHAMIRVQLRRQGITVSKIRRHYSRDRDAGKSITALDITLFTRQLATLIS
AGIALQQALGVIADGFESRAMRQQVTELKQDIGAGTSFTAALRKKPAHFDPLFCSLVEAGELAGALDVMLQRVATYREKT
EALKRKIKKAMTYPSAVLLVALGVCSILLIAVVPQFQGVFRSFDAQLPALTLWVIGLSDGLQAHGLWLLCMSGVILAGLH
HRYKHCPALRQRIDRWLLHLPVLGNLLHMSAIARFARTLSTTLAAGVPLLEALNSVASATRNNLHKDAVLRLKQQVATGS
PIHVAMAAVGVFPSMAIQMTAIGEESGTLDSMLDHVAAYYESEVDTQVDHLTTLLEPIIMVVLGVMVGGLVIAMYLPIFN
LGQVI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1081022 ACKZH3_RS04085 WP_088378957.1 852535..853752(-) (pilC) [Pseudomonas fragi strain LKYC.Zb1]
ATGGCAACACAGGCCGTTAAAAACACCCTCTACCGCTGGCAAGGCACAGATGGCAACGGCGTTAAAACCAGCGGCCAGAT
CTCGGCTGACAGCCACGCCATGATCAGGGTCCAACTACGCCGGCAAGGTATTACCGTTAGTAAAATCCGCCGCCATTACA
GTCGCGATCGCGATGCAGGCAAATCCATCACGGCGCTGGATATCACCCTGTTCACCCGCCAGTTGGCAACCCTGATCAGC
GCCGGTATTGCCTTGCAGCAAGCCCTGGGGGTGATCGCCGACGGTTTCGAGAGCCGGGCCATGCGCCAGCAGGTCACCGA
ACTCAAACAGGACATCGGCGCAGGCACCAGCTTTACGGCAGCACTGCGCAAAAAACCGGCACATTTCGACCCGCTGTTTT
GCAGCCTCGTGGAAGCGGGGGAACTGGCCGGGGCGCTGGACGTCATGCTGCAACGGGTGGCGACCTACAGGGAAAAAACC
GAAGCCCTGAAAAGGAAAATCAAAAAAGCCATGACCTATCCCAGCGCCGTGCTCTTGGTGGCGCTGGGGGTGTGCAGTAT
TTTGCTGATCGCAGTGGTGCCGCAGTTTCAGGGCGTGTTCCGCTCATTCGATGCGCAACTGCCGGCGCTGACCTTGTGGG
TCATCGGCCTGTCTGATGGCTTGCAGGCACATGGCCTGTGGCTGCTGTGTATGAGCGGCGTCATTCTGGCCGGCCTTCAT
CATCGCTACAAACATTGCCCAGCCTTGCGCCAGCGCATTGATCGCTGGCTGCTGCATTTGCCCGTCCTGGGCAATCTGCT
GCACATGTCAGCCATCGCCCGTTTTGCCCGTACGCTGTCGACCACCCTGGCTGCCGGGGTGCCATTGCTCGAAGCGCTGA
ACAGTGTCGCCAGCGCCACGCGCAATAACCTGCACAAGGACGCGGTGCTGCGGCTCAAACAACAGGTTGCGACTGGTAGC
CCGATCCATGTGGCAATGGCTGCCGTCGGAGTATTCCCGAGCATGGCAATTCAGATGACAGCAATTGGCGAAGAATCCGG
CACATTAGACAGCATGCTGGACCACGTTGCCGCCTATTATGAAAGCGAGGTCGACACACAGGTCGACCACCTGACAACGT
TGCTGGAGCCGATCATCATGGTGGTACTTGGAGTCATGGTTGGCGGGCTGGTCATCGCCATGTACTTGCCGATCTTCAAC
CTGGGCCAGGTCATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.317

100

0.536
  pilC Acinetobacter baumannii D1279779

50.126

98.025

0.491
  pilC Acinetobacter baylyi ADP1

48.995

98.272

0.481
  pilC Legionella pneumophila strain ERS1305867

48.615

98.025

0.477
  pilC Vibrio cholerae strain A1552

41.975

100

0.42
  pilC Vibrio campbellii strain DS40M4

40.049

100

0.407
  pilC Thermus thermophilus HB27

37.5

98.765

0.37
  pilG Neisseria gonorrhoeae MS11

38.743

94.321

0.365
  pilG Neisseria meningitidis 44/76-A

38.482

94.321

0.363

Multiple sequence alignment