Detailed information NTG1081021

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACKZH3_RS04080
• Genome accession: NZ_CP176572
• Coordinates: 851672..852532 (-)
• Length: 286 a.a.
• NCBI ID: WP_409498338.1
• Uniprot ID: -
• Organism: Pseudomonas fragi strain LKYC.Zb1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 846672..857532

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACKZH3_RS04045 (ACKZH3_04045)	-	847198..848499 (-)	1302	WP_088378951.1	NAD(P)/FAD-dependent oxidoreductase	-
ACKZH3_RS04050 (ACKZH3_04050)	-	848566..848739 (-)	174	WP_088378952.1	DUF3094 family protein	-
ACKZH3_RS04055 (ACKZH3_04055)	-	848794..849321 (-)	528	WP_409498337.1	MOSC domain-containing protein	-
ACKZH3_RS04060 (ACKZH3_04060)	-	849318..849947 (-)	630	WP_088378953.1	DUF1780 domain-containing protein	-
ACKZH3_RS04065 (ACKZH3_04065)	-	850057..850743 (+)	687	WP_088378954.1	energy-coupling factor ABC transporter permease	-
ACKZH3_RS04070 (ACKZH3_04070)	yacG	850847..851050 (-)	204	WP_016779444.1	DNA gyrase inhibitor YacG	-
ACKZH3_RS04075 (ACKZH3_04075)	coaE	851047..851670 (-)	624	WP_088378955.1	dephospho-CoA kinase	-
ACKZH3_RS04080 (ACKZH3_04080)	pilD	851672..852532 (-)	861	WP_409498338.1	prepilin peptidase	Machinery gene
ACKZH3_RS04085 (ACKZH3_04085)	pilC	852535..853752 (-)	1218	WP_088378957.1	type II secretion system F family protein	Machinery gene
ACKZH3_RS04090 (ACKZH3_04090)	-	853964..854371 (+)	408	WP_088378958.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
ACKZH3_RS04100 (ACKZH3_04100)	-	854664..856154 (+)	1491	WP_409498339.1	AlkA N-terminal domain-containing protein	-
ACKZH3_RS04105 (ACKZH3_04105)	-	856157..856648 (+)	492	WP_409498340.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31320.25 Da
• Isoelectric Point: 8.5946

>NTDB_id=1081021 ACKZH3_RS04080 WP_409498338.1 851672..852532(-) (pilD) [Pseudomonas fragi strain LKYC.Zb1]
MIELLSHHPDAFIACALILGLLVGSFINVLAWRLPQMLERDWQAQARDILGLPEPAKGPVYNLLRPRSHCPNCQHSLRAR
ENIPLLSYLFLRGKCSHCKTPISLRYPLTELACAALSAFVAWHFGFGAQAGWMLVLTWGLLAICLIDAEHQIVPDVLVFP
LLWLGLLLNSFSLFTTLGQAVWGAALGYASLWSVFWMFKLITGKDGMGYGDFKLLAVLGAWGGVAVLPLTILLSSLLGAA
AGVGLLLLRRARTSTPIPFAPYLAIAGWIALLWGGQITGFYWQVSV

Nucleotide

• Length: 861 bp

>NTDB_id=1081021 ACKZH3_RS04080 WP_409498338.1 851672..852532(-) (pilD) [Pseudomonas fragi strain LKYC.Zb1]
ATGATTGAATTGCTGAGCCATCACCCCGACGCATTTATCGCCTGCGCCCTGATTCTCGGCTTGCTGGTGGGCAGCTTTAT
CAATGTACTGGCGTGGCGCTTGCCCCAGATGCTTGAACGGGACTGGCAGGCCCAGGCGCGCGACATCCTGGGCTTGCCCG
AGCCGGCCAAGGGCCCTGTGTATAACCTGCTGCGCCCGCGCTCCCACTGCCCCAATTGCCAGCATTCACTGCGTGCCCGG
GAGAACATTCCGCTGCTGAGCTACCTGTTTTTACGCGGCAAGTGCTCACACTGCAAAACCCCGATCAGCCTGCGTTACCC
CCTTACTGAGCTGGCCTGCGCGGCATTGTCGGCTTTTGTTGCCTGGCATTTCGGTTTTGGCGCCCAGGCGGGCTGGATGC
TGGTGCTGACCTGGGGGTTACTCGCCATTTGCCTGATCGATGCCGAGCATCAAATCGTGCCCGACGTGCTGGTTTTCCCG
CTGCTCTGGCTGGGCTTGTTGCTCAACAGCTTCTCCCTGTTCACCACACTGGGGCAGGCCGTATGGGGCGCAGCGCTGGG
CTATGCAAGCCTGTGGTCGGTGTTCTGGATGTTCAAACTGATCACCGGCAAGGACGGCATGGGCTACGGCGACTTCAAGC
TACTGGCCGTGCTCGGCGCCTGGGGCGGGGTTGCGGTTTTGCCGCTGACCATCCTGCTGTCTTCACTGCTGGGGGCCGCT
GCAGGGGTTGGGCTGTTGCTCTTGCGCCGGGCCAGGACCAGTACCCCGATCCCCTTTGCACCGTATCTGGCAATTGCCGG
CTGGATTGCATTGCTCTGGGGTGGTCAAATAACCGGCTTCTATTGGCAGGTTTCGGTTTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (134-242)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-124)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	47.931	100	0.486
pilD	Acinetobacter baumannii D1279779	48.339	94.755	0.458
pilD	Acinetobacter nosocomialis M2	48.339	94.755	0.458
pilD	Vibrio campbellii strain DS40M4	44.912	99.65	0.448
pilD	Neisseria gonorrhoeae MS11	46.275	89.161	0.413