Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACKUBV_RS14550 Genome accession   NZ_CP176357
Coordinates   3052740..3053963 (-) Length   407 a.a.
NCBI ID   WP_005493981.1    Uniprot ID   A0A249W378
Organism   Vibrio parahaemolyticus strain AG1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3047740..3058963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKUBV_RS14510 (ACKUBV_14510) rpsP 3047820..3048068 (+) 249 WP_005379962.1 30S ribosomal protein S16 -
  ACKUBV_RS14515 (ACKUBV_14515) rimM 3048097..3048645 (+) 549 WP_047123098.1 ribosome maturation factor RimM -
  ACKUBV_RS14520 (ACKUBV_14520) trmD 3048673..3049416 (+) 744 WP_047123099.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  ACKUBV_RS14525 (ACKUBV_14525) rplS 3049458..3049811 (+) 354 WP_005462554.1 50S ribosomal protein L19 -
  ACKUBV_RS14530 (ACKUBV_14530) yacG 3050160..3050354 (-) 195 WP_005462546.1 DNA gyrase inhibitor YacG -
  ACKUBV_RS14535 (ACKUBV_14535) zapD 3050423..3051163 (-) 741 WP_005480890.1 cell division protein ZapD -
  ACKUBV_RS14540 (ACKUBV_14540) coaE 3051191..3051805 (-) 615 WP_005480887.1 dephospho-CoA kinase -
  ACKUBV_RS14545 (ACKUBV_14545) pilD 3051806..3052675 (-) 870 WP_025533026.1 prepilin peptidase Machinery gene
  ACKUBV_RS14550 (ACKUBV_14550) pilC 3052740..3053963 (-) 1224 WP_005493981.1 type II secretion system F family protein Machinery gene
  ACKUBV_RS14555 (ACKUBV_14555) pilB 3053987..3055672 (-) 1686 WP_069536419.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACKUBV_RS14560 (ACKUBV_14560) pilA 3055678..3056151 (-) 474 WP_069536420.1 pilin Machinery gene
  ACKUBV_RS14565 (ACKUBV_14565) nadC 3056415..3057302 (-) 888 WP_015297249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACKUBV_RS14570 (ACKUBV_14570) ampD 3057395..3057946 (+) 552 WP_025554880.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45208.33 Da        Isoelectric Point: 10.4368

>NTDB_id=1079732 ACKUBV_RS14550 WP_005493981.1 3052740..3053963(-) (pilC) [Vibrio parahaemolyticus strain AG1]
MKTTTPQLKNFRWKGVNSSGKKTSGQTLAMSETEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGKDITVFTRQISTML
VTGVPLVQALKLVSDNHKKAEMKSILMSVTRAVEAGTPMSKAMRTASNHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVILVALGVSFIMLTKVIPEFEKMFVGFGAELPWFTRQVLDLSAWTQNWSPFIALGSISVFISA
RVLSKRSDSFRLMLNRSVLKFPVLGAVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=1079732 ACKUBV_RS14550 WP_005493981.1 3052740..3053963(-) (pilC) [Vibrio parahaemolyticus strain AG1]
ATGAAAACTACGACACCACAATTAAAGAACTTCCGTTGGAAAGGCGTCAACAGCTCAGGGAAAAAGACCTCTGGTCAAAC
TCTGGCAATGAGCGAAACCGAAGTACGAGAGCGCTTAGATGCCCAGCACATCAAAATCAAAAAGCTAAAGAAAAGCAGCA
TCTCTTTTTTGACCAAACTTAGCCATCGCGTAAAAGGAAAAGACATCACGGTTTTCACTCGTCAAATCTCAACGATGCTA
GTAACCGGTGTGCCGCTAGTACAAGCATTGAAATTGGTCTCTGATAACCACAAAAAGGCGGAAATGAAATCCATTTTGAT
GAGTGTAACTCGGGCTGTTGAAGCGGGTACGCCGATGTCCAAAGCGATGCGTACCGCCAGTAACCATTTTGACCCGCTGT
ATACCGACCTGATTGCGACAGGAGAACAATCGGGCAACTTAGCCGAAGTGTTCGAACGCTTAGCCACCTACAGGGAAAAA
AATGAGCAGCTTAGAGCCAAAGTGATCAAGGCTCTAATATATCCAGCCATGGTTATTCTAGTTGCACTGGGAGTATCGTT
TATCATGCTAACCAAGGTCATTCCCGAGTTTGAAAAAATGTTTGTTGGCTTTGGTGCTGAACTGCCATGGTTTACAAGGC
AAGTCTTAGATCTTTCCGCTTGGACACAAAACTGGAGCCCATTTATCGCACTTGGTTCCATCAGTGTATTTATCTCCGCG
AGAGTACTCTCTAAGCGTTCAGATTCTTTTCGTTTAATGCTCAACCGATCTGTACTTAAGTTTCCAGTCCTTGGGGCGGT
TTTATCGAAAGCCGCTATCGCAAAATTTAGTCGAACCCTCGCTACGAGCTTTACGGCGGGCATTCCGATTCTAACCAGTT
TAAAAACCACATCGAAAACGTCCGGAAATATGCATTATCAATTGGCTATCGAAGAGGTTTATCGCGATACCGCAGCAGGA
ATGCCGATGTATGTTGCAATGCGCAATTGCAACGTGTTCCCTGAGTTGGTGCTGCAAATGGTTATGATCGGCGAAGAATC
CGGCCGACTTGACGATATGCTCAATAAAGTCGCCACAATTTACGAGTTTGAAGTGGACAACACCGTCGATAACCTCAGTA
AAATCTTAGAACCATTAATTATCGTTTTTTTAGGTATCGTTGTCGGTGGCCTGGTCACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249W378

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

85.749

100

0.857
  pilC Vibrio cholerae strain A1552

74.074

99.509

0.737
  pilC Acinetobacter baylyi ADP1

43

98.28

0.423
  pilC Acinetobacter baumannii D1279779

41.439

99.017

0.41
  pilG Neisseria gonorrhoeae MS11

40.494

99.509

0.403
  pilG Neisseria meningitidis 44/76-A

40.247

99.509

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398
  pilC Legionella pneumophila strain ERS1305867

39.803

100

0.398

Multiple sequence alignment