Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACKUBV_RS14560 Genome accession   NZ_CP176357
Coordinates   3055678..3056151 (-) Length   157 a.a.
NCBI ID   WP_069536420.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain AG1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3050678..3061151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKUBV_RS14540 (ACKUBV_14540) coaE 3051191..3051805 (-) 615 WP_005480887.1 dephospho-CoA kinase -
  ACKUBV_RS14545 (ACKUBV_14545) pilD 3051806..3052675 (-) 870 WP_025533026.1 prepilin peptidase Machinery gene
  ACKUBV_RS14550 (ACKUBV_14550) pilC 3052740..3053963 (-) 1224 WP_005493981.1 type II secretion system F family protein Machinery gene
  ACKUBV_RS14555 (ACKUBV_14555) pilB 3053987..3055672 (-) 1686 WP_069536419.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACKUBV_RS14560 (ACKUBV_14560) pilA 3055678..3056151 (-) 474 WP_069536420.1 pilin Machinery gene
  ACKUBV_RS14565 (ACKUBV_14565) nadC 3056415..3057302 (-) 888 WP_015297249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACKUBV_RS14570 (ACKUBV_14570) ampD 3057395..3057946 (+) 552 WP_025554880.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACKUBV_RS14575 (ACKUBV_14575) pdhR 3058352..3059119 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16666.33 Da        Isoelectric Point: 9.1138

>NTDB_id=1079734 ACKUBV_RS14560 WP_069536420.1 3055678..3056151(-) (pilA) [Vibrio parahaemolyticus strain AG1]
MKHRKQKKQQGFTLIELMIVVAIIGILSAFAVPAYQNYTKKATLSEFPKAAAAAKLAVELCAHENASNETTFISECVKKE
NGIPAKFVLNNIEISVVKATDASVSGAIAVIAAADQVKGPIAKGEQYIMAASYKTQGLEWGASCKAGGKAQTDYCPN

Nucleotide


Download         Length: 474 bp        

>NTDB_id=1079734 ACKUBV_RS14560 WP_069536420.1 3055678..3056151(-) (pilA) [Vibrio parahaemolyticus strain AG1]
ATGAAACACCGTAAACAGAAAAAACAGCAAGGTTTTACGCTAATTGAATTGATGATTGTGGTGGCGATTATCGGCATCTT
ATCTGCTTTCGCAGTTCCTGCTTACCAAAATTATACTAAGAAAGCGACATTGTCAGAATTTCCAAAAGCAGCTGCGGCAG
CCAAATTAGCAGTAGAATTATGTGCCCATGAGAATGCCTCAAATGAAACAACATTTATTAGTGAATGTGTAAAGAAAGAA
AATGGAATTCCCGCTAAATTCGTTTTGAATAATATTGAAATTAGCGTTGTTAAAGCCACAGATGCTAGCGTTTCTGGTGC
GATAGCAGTTATTGCCGCAGCTGATCAAGTTAAGGGACCTATTGCTAAAGGCGAACAATATATCATGGCTGCATCATATA
AAACTCAAGGCTTAGAGTGGGGGGCTTCATGTAAAGCTGGCGGTAAAGCACAAACAGACTACTGCCCAAACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

56.051

100

0.561
  pilA Haemophilus influenzae Rd KW20

42.361

91.72

0.389
  pilA Haemophilus influenzae 86-028NP

41.379

92.357

0.382

Multiple sequence alignment