Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   AACH74_RS02210 Genome accession   NZ_AP029000
Coordinates   453796..454920 (+) Length   374 a.a.
NCBI ID   WP_000228508.1    Uniprot ID   A0A0D8VMA7
Organism   Escherichia coli strain GU2018_CL13     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 448796..459920
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH74_RS02185 (GU2018CL13_04320) mscL 448913..449326 (-) 414 WP_000022450.1 large-conductance mechanosensitive channel protein MscL -
  AACH74_RS02190 (GU2018CL13_04330) trkA 449456..450832 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  AACH74_RS02195 (GU2018CL13_04340) rsmB 450854..452143 (-) 1290 WP_000744766.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  AACH74_RS02200 (GU2018CL13_04350) fmt 452195..453142 (-) 948 WP_000004421.1 methionyl-tRNA formyltransferase -
  AACH74_RS02205 (GU2018CL13_04360) def 453157..453666 (-) 510 WP_000114984.1 peptide deformylase -
  AACH74_RS02210 (GU2018CL13_04370) dprA 453796..454920 (+) 1125 WP_000228508.1 DNA-protecting protein DprA Machinery gene
  AACH74_RS02215 (GU2018CL13_04380) smg 454892..455365 (+) 474 WP_000460672.1 DUF494 family protein Smg -
  AACH74_RS02220 yrdD 455394..455936 (+) 543 WP_001312137.1 type I DNA topoisomerase -
  AACH74_RS02225 (GU2018CL13_04400) tsaC 455941..456513 (+) 573 WP_001297709.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  AACH74_RS02230 (GU2018CL13_04410) aroE 456518..457336 (+) 819 WP_000451230.1 shikimate dehydrogenase -
  AACH74_RS02235 (GU2018CL13_04420) yrdB 457333..457590 (+) 258 WP_001070567.1 DUF1488 domain-containing protein -
  AACH74_RS02240 (GU2018CL13_04430) yrdA 457566..458120 (-) 555 WP_001286216.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40892.82 Da        Isoelectric Point: 6.0395

>NTDB_id=107935 AACH74_RS02210 WP_000228508.1 453796..454920(+) (dprA) [Escherichia coli strain GU2018_CL13]
MVDTEIWLRLISISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=107935 AACH74_RS02210 WP_000228508.1 453796..454920(+) (dprA) [Escherichia coli strain GU2018_CL13]
ATGGTTGATACAGAAATTTGGCTGCGTTTAATTAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT
TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (DprA_WH)

(312-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D8VMA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Haemophilus influenzae Rd KW20

43.917

90.107

0.396
  dprA Legionella pneumophila strain ERS1305867

44.242

88.235

0.39

Multiple sequence alignment