Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACJZU6_RS03630 Genome accession   NZ_CP175534
Coordinates   747633..749318 (-) Length   561 a.a.
NCBI ID   WP_408900656.1    Uniprot ID   -
Organism   Photobacterium piscicola strain WVL24019     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 742633..754318
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJZU6_RS03600 (ACJZU6_03600) mutT 743280..743687 (-) 408 WP_080158398.1 8-oxo-dGTP diphosphatase MutT -
  ACJZU6_RS03605 (ACJZU6_03605) yacG 743820..744020 (-) 201 WP_080158306.1 DNA gyrase inhibitor YacG -
  ACJZU6_RS03610 (ACJZU6_03610) zapD 744028..744765 (-) 738 WP_080158305.1 cell division protein ZapD -
  ACJZU6_RS03615 (ACJZU6_03615) coaE 744812..745417 (-) 606 WP_080158304.1 dephospho-CoA kinase -
  ACJZU6_RS03620 (ACJZU6_03620) pilD 745421..746296 (-) 876 WP_408900655.1 prepilin peptidase Machinery gene
  ACJZU6_RS03625 (ACJZU6_03625) pilC 746360..747580 (-) 1221 WP_107276463.1 type II secretion system F family protein Machinery gene
  ACJZU6_RS03630 (ACJZU6_03630) pilB 747633..749318 (-) 1686 WP_408900656.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJZU6_RS03635 (ACJZU6_03635) pilA 749318..749719 (-) 402 WP_408900657.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACJZU6_RS03640 (ACJZU6_03640) nadC 750089..750976 (-) 888 WP_408900913.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACJZU6_RS03645 (ACJZU6_03645) ampD 751363..751908 (+) 546 WP_408900658.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACJZU6_RS03650 (ACJZU6_03650) pdhR 752216..752989 (+) 774 WP_080158298.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62333.76 Da        Isoelectric Point: 4.9122

>NTDB_id=1077916 ACJZU6_RS03630 WP_408900656.1 747633..749318(-) (pilB) [Photobacterium piscicola strain WVL24019]
MQTNLASILFQAELISAEQEQQIVESVTAEGINVPTAITRLGIMADDELARSLSHLFNLDRVDVHHYDFQGCCQSVYQRE
LVLRHRVLPLAQDNSTLFVGLSDPTNVEAQDEFRFAIGKNVEALLLDNKQLESAIRRVYGSDIGDVGNSRQISDEDLGNL
IDINDIELDEATDLSQDSAPVTRYINQVLMDALRRKASDIHFEPYETSYRIRFRCDGILHQHLTPPASLSRRLSTRLKVM
SKLNIAERRQPQDGRIKLKLSDSLAIDLRVSTLPTLWGEKVVLRILDSSSASLNIDILGYSDAQKQAYLTALQRPQGMIL
MTGPTGSGKTVSLYTGLSILNTDERNISTAEDPVEINLQGINQVQINNQAGLGFAEALRSFLRQDPDVVMVGEIRDLETA
EIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGVEDFNLASSLSLIIAQRLARRLCSHCKQPHLLDDLTCERLKLPLNTS
VYQAHKEGCDDCNKGYVGRVGIYEVMQFSREIAEALMQNATSLQLEDIAASQGMQRLRESGVDKLVEGITSLVELQRVLQ
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1077916 ACJZU6_RS03630 WP_408900656.1 747633..749318(-) (pilB) [Photobacterium piscicola strain WVL24019]
ATGCAAACAAACCTCGCCTCGATCCTGTTCCAAGCTGAGTTAATCAGCGCCGAACAAGAGCAACAAATTGTCGAGTCGGT
GACGGCGGAAGGGATTAATGTCCCGACCGCCATTACTCGTCTAGGAATCATGGCGGATGATGAACTAGCACGTTCATTAT
CACACCTGTTCAATCTTGATCGGGTTGATGTCCACCACTATGACTTTCAAGGTTGCTGTCAAAGTGTCTACCAACGTGAA
CTGGTGTTACGCCATCGCGTGCTGCCGCTTGCGCAAGACAATAGCACCCTTTTCGTCGGCCTTAGCGATCCTACCAATGT
CGAGGCGCAAGATGAATTTCGCTTCGCTATTGGTAAGAACGTTGAAGCACTATTATTAGACAACAAACAACTTGAATCGG
CGATTCGCCGAGTTTACGGTAGTGATATCGGTGATGTTGGTAACAGCCGCCAAATCAGTGATGAAGATTTAGGTAACTTA
ATTGATATCAACGATATTGAACTTGATGAAGCCACCGATTTAAGCCAAGACAGCGCTCCGGTTACTCGTTATATCAACCA
AGTATTAATGGATGCTCTGCGCCGTAAAGCATCCGACATTCACTTTGAACCCTATGAAACCAGCTATCGAATTCGGTTTC
GTTGTGATGGTATTTTGCATCAGCATTTAACACCACCAGCCAGTTTATCGCGGCGGTTATCAACCCGTTTAAAGGTAATG
TCCAAGCTCAATATTGCCGAACGTCGTCAACCACAAGATGGCCGCATCAAACTTAAATTATCCGATTCACTGGCAATTGA
TCTGCGAGTTTCTACCCTACCAACCTTGTGGGGCGAAAAAGTAGTACTGCGTATTTTAGACAGTTCCAGTGCCAGCTTAA
ACATTGATATTTTAGGGTATAGCGACGCTCAAAAGCAGGCTTATTTAACCGCGCTGCAACGCCCGCAAGGGATGATTTTA
ATGACTGGCCCCACTGGTAGTGGCAAAACCGTGTCGCTCTATACCGGTTTAAGTATTTTAAACACTGACGAGCGCAATAT
TTCTACCGCCGAAGATCCGGTTGAGATCAACCTCCAAGGTATCAACCAAGTTCAAATCAATAATCAGGCTGGACTTGGAT
TTGCTGAAGCATTGCGATCGTTTCTACGTCAAGACCCAGATGTAGTAATGGTCGGTGAAATTCGTGATCTTGAAACCGCC
GAAATTGCGGTTAAAGCGGCGCAAACTGGGCACTTGGTGTTATCAACCCTGCACACCAACTCAGCTGCGGAAACCATTAC
CCGTTTAACCAACATGGGCGTCGAAGATTTTAACCTTGCCTCATCACTGAGTTTAATTATTGCGCAGCGGTTAGCACGTC
GATTATGCAGTCACTGTAAGCAACCGCATCTACTTGATGACTTAACTTGTGAACGCTTAAAACTGCCACTTAACACTAGT
GTTTATCAAGCCCATAAAGAAGGCTGTGATGATTGCAACAAAGGCTATGTTGGCCGAGTGGGTATTTATGAAGTGATGCA
ATTTAGCCGTGAAATTGCTGAAGCATTAATGCAAAACGCCACCAGCTTACAATTAGAAGATATTGCTGCCAGCCAAGGCA
TGCAACGACTACGCGAATCAGGAGTAGATAAATTAGTTGAAGGCATTACTAGCCTTGTCGAATTACAACGGGTACTACAA
CTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-454)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

63.393

99.822

0.633
  pilB Vibrio parahaemolyticus RIMD 2210633

60.179

99.822

0.601
  pilB Vibrio campbellii strain DS40M4

59.464

99.822

0.594
  pilB Acinetobacter baumannii D1279779

53.179

92.513

0.492
  pilB Legionella pneumophila strain ERS1305867

49.541

97.148

0.481
  pilB Acinetobacter baylyi ADP1

51.644

92.157

0.476
  pilF Neisseria gonorrhoeae MS11

45.471

100

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

39.729

91.979

0.365
  pilF Thermus thermophilus HB27

36.106

100

0.364

Multiple sequence alignment