Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACJJWD_RS04220 Genome accession   NZ_CP174505
Coordinates   928891..930108 (-) Length   405 a.a.
NCBI ID   WP_407715145.1    Uniprot ID   -
Organism   Comamonas testosteroni strain VSPL.dLG     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 923891..935108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJJWD_RS04190 (ACJJWD_04195) - 924699..925634 (+) 936 WP_034348267.1 ATP-binding protein -
  ACJJWD_RS04195 (ACJJWD_04200) - 925647..926117 (+) 471 WP_343676024.1 NUDIX domain-containing protein -
  ACJJWD_RS04200 (ACJJWD_04205) - 926235..926444 (-) 210 WP_407715142.1 DNA gyrase inhibitor YacG -
  ACJJWD_RS04205 (ACJJWD_04210) zapD 926543..927298 (-) 756 WP_003058549.1 cell division protein ZapD -
  ACJJWD_RS04210 (ACJJWD_04215) coaE 927386..928012 (-) 627 WP_407715143.1 dephospho-CoA kinase -
  ACJJWD_RS04215 (ACJJWD_04220) - 928016..928891 (-) 876 WP_407715144.1 prepilin peptidase -
  ACJJWD_RS04220 (ACJJWD_04225) pilC 928891..930108 (-) 1218 WP_407715145.1 type II secretion system F family protein Machinery gene
  ACJJWD_RS04225 (ACJJWD_04230) pilB 930133..931875 (-) 1743 WP_407715146.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJJWD_RS04235 (ACJJWD_04240) - 932130..933059 (-) 930 WP_407715147.1 polyprenyl synthetase family protein -
  ACJJWD_RS04240 (ACJJWD_04245) rplU 933394..933705 (+) 312 WP_003058536.1 50S ribosomal protein L21 -
  ACJJWD_RS04245 (ACJJWD_04250) rpmA 933722..933979 (+) 258 WP_003058534.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44471.18 Da        Isoelectric Point: 9.6093

>NTDB_id=1077448 ACJJWD_RS04220 WP_407715145.1 928891..930108(-) (pilC) [Comamonas testosteroni strain VSPL.dLG]
MASAASKGIKEFLFEWEGKDRNGKIVRGETRAGGENQIQAMLRRQGVTPSKIKKRRTRGGKKIKPKDIALFTRQLATMMK
AGVPLLQSFDIVGRGNTNPNATKLLNDIRSDVETGTSLSAAFRKFPLYFNSLYCNLVEAGEAAGILESLLDRLATYMEKT
EAIKSKIKSALMYPTSVMIVAFVVVTVIMIFVIPAFKEVFTSFGADLPAPTLLVMGISDYFVQYWWLIFGVLGGGIYFFM
QAWKRNERVQQFMDRTVLKLPIFGVLIEKSCVARWTRTLSTMFAAGVPLVEALDSVGGASGNYLYKNATDRIQSEVSTGT
SLTVAMANANIFPSMVLQMCAIGEESGAIDHMLGKAADFYESEVDEMVAGLSSLMEPIIIVFLGTLIGGIVVSMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1077448 ACJJWD_RS04220 WP_407715145.1 928891..930108(-) (pilC) [Comamonas testosteroni strain VSPL.dLG]
ATGGCAAGCGCAGCGTCCAAGGGAATCAAGGAATTTCTCTTCGAGTGGGAAGGCAAGGACCGTAACGGCAAGATCGTTCG
CGGTGAAACGCGTGCCGGTGGAGAAAACCAGATCCAGGCCATGCTGCGCCGCCAGGGGGTGACTCCGTCCAAGATCAAGA
AGCGCAGGACCCGTGGCGGCAAGAAGATCAAGCCCAAGGACATTGCACTCTTCACGCGCCAGCTGGCCACCATGATGAAG
GCCGGCGTGCCGCTGCTGCAGTCTTTCGACATTGTGGGTCGCGGCAATACCAATCCTAATGCCACCAAGCTGCTCAACGA
CATCCGCTCCGATGTGGAGACCGGCACCTCGCTGAGCGCTGCCTTCCGCAAGTTTCCGCTCTATTTCAACAGCCTCTACT
GCAACCTGGTGGAGGCCGGCGAGGCCGCAGGTATTCTGGAATCGCTACTGGACCGCCTTGCCACCTACATGGAGAAGACG
GAAGCCATCAAGTCCAAGATCAAGTCGGCGCTGATGTACCCTACATCGGTCATGATCGTGGCCTTTGTGGTGGTGACCGT
GATCATGATCTTCGTGATCCCGGCCTTCAAGGAGGTCTTCACCTCCTTCGGTGCCGACCTGCCCGCGCCCACCTTGCTGG
TGATGGGTATCAGCGATTACTTTGTCCAGTACTGGTGGCTGATCTTCGGCGTGCTGGGCGGCGGCATCTACTTCTTCATG
CAGGCCTGGAAGCGCAACGAGCGCGTGCAGCAGTTCATGGACCGCACCGTGCTCAAGCTACCCATCTTCGGCGTGCTGAT
CGAGAAATCCTGCGTGGCCCGCTGGACGCGCACACTGTCCACCATGTTTGCAGCCGGCGTGCCGCTGGTTGAGGCGCTGG
ACTCCGTGGGCGGCGCCTCGGGCAACTACCTCTACAAAAACGCCACCGACAGGATTCAGTCGGAGGTTTCCACGGGCACC
AGCCTCACGGTTGCCATGGCCAATGCCAATATCTTCCCTTCCATGGTGCTGCAGATGTGCGCCATCGGCGAGGAATCGGG
CGCCATCGACCATATGCTGGGCAAGGCCGCCGACTTCTATGAAAGTGAAGTGGACGAAATGGTGGCGGGTCTGTCCAGCC
TGATGGAGCCCATCATCATCGTCTTCCTGGGCACCTTGATCGGCGGCATCGTGGTGTCCATGTATCTGCCTATCTTCAAG
TTGGGTCAAGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.465

99.753

0.533
  pilG Neisseria gonorrhoeae MS11

51.889

98.025

0.509
  pilG Neisseria meningitidis 44/76-A

51.637

98.025

0.506
  pilC Legionella pneumophila strain ERS1305867

50.758

97.778

0.496
  pilC Acinetobacter baylyi ADP1

50

98.272

0.491
  pilC Acinetobacter baumannii D1279779

48.363

98.025

0.474
  pilC Vibrio cholerae strain A1552

40.302

98.025

0.395
  pilC Vibrio campbellii strain DS40M4

38.06

99.259

0.378
  pilC Thermus thermophilus HB27

36.634

99.753

0.365

Multiple sequence alignment