Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACJJWD_RS04225 Genome accession   NZ_CP174505
Coordinates   930133..931875 (-) Length   580 a.a.
NCBI ID   WP_407715146.1    Uniprot ID   -
Organism   Comamonas testosteroni strain VSPL.dLG     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 925133..936875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJJWD_RS04195 (ACJJWD_04200) - 925647..926117 (+) 471 WP_343676024.1 NUDIX domain-containing protein -
  ACJJWD_RS04200 (ACJJWD_04205) - 926235..926444 (-) 210 WP_407715142.1 DNA gyrase inhibitor YacG -
  ACJJWD_RS04205 (ACJJWD_04210) zapD 926543..927298 (-) 756 WP_003058549.1 cell division protein ZapD -
  ACJJWD_RS04210 (ACJJWD_04215) coaE 927386..928012 (-) 627 WP_407715143.1 dephospho-CoA kinase -
  ACJJWD_RS04215 (ACJJWD_04220) - 928016..928891 (-) 876 WP_407715144.1 prepilin peptidase -
  ACJJWD_RS04220 (ACJJWD_04225) pilC 928891..930108 (-) 1218 WP_407715145.1 type II secretion system F family protein Machinery gene
  ACJJWD_RS04225 (ACJJWD_04230) pilB 930133..931875 (-) 1743 WP_407715146.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJJWD_RS04235 (ACJJWD_04240) - 932130..933059 (-) 930 WP_407715147.1 polyprenyl synthetase family protein -
  ACJJWD_RS04240 (ACJJWD_04245) rplU 933394..933705 (+) 312 WP_003058536.1 50S ribosomal protein L21 -
  ACJJWD_RS04245 (ACJJWD_04250) rpmA 933722..933979 (+) 258 WP_003058534.1 50S ribosomal protein L27 -
  ACJJWD_RS04250 (ACJJWD_04255) cgtA 934136..935251 (+) 1116 WP_043003501.1 Obg family GTPase CgtA -
  ACJJWD_RS04255 (ACJJWD_04260) proB 935362..936501 (+) 1140 WP_167006277.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 63043.31 Da        Isoelectric Point: 5.1585

>NTDB_id=1077449 ACJJWD_RS04225 WP_407715146.1 930133..931875(-) (pilB) [Comamonas testosteroni strain VSPL.dLG]
MAAADNLQKNAPAPIAIPGLGRALISAGKVSQLAAENAAKKAATGKTPFITELSQSGEISALEIAETISMMFSTPLLDLN
AIDAQQLPRELLDPKISSAYRVLALSKRGNRLTIATADPTQQEAAEQIKFTTQLLVDWVVVEADKLQKLVEQTGKSVSES
LDSYSSSGDFDFDDVKIEDAPEEKDNAVGAEVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHNYRVRFRIDGELREIAS
PPIAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KERLLKAINRPYGMILVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINMPGVNQVNVNEKAGLTFAAALKSFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCAQCKEPAD
IPHDALIEAGYAEEELDGSWVSYKPVGCPACNNGYKGRVGIYQVMPISEEIQRIILRDGSALEIAAQAKAEGVRSLRGSG
LHKVKLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=1077449 ACJJWD_RS04225 WP_407715146.1 930133..931875(-) (pilB) [Comamonas testosteroni strain VSPL.dLG]
ATGGCCGCTGCCGACAATCTGCAAAAGAACGCTCCTGCGCCCATTGCCATCCCCGGTCTAGGCAGAGCCCTGATTTCCGC
CGGCAAGGTCTCGCAGCTAGCCGCCGAGAACGCAGCCAAGAAAGCAGCCACAGGCAAGACACCATTCATCACCGAACTGA
GCCAGTCCGGCGAGATCAGCGCGCTGGAAATTGCCGAAACCATCTCCATGATGTTCAGCACACCGCTGCTGGACCTCAAT
GCCATCGACGCCCAGCAGCTGCCACGCGAGCTGCTGGACCCCAAGATCAGCAGCGCCTACCGCGTGCTGGCGCTCAGCAA
GCGCGGCAACCGGCTCACCATTGCCACGGCAGACCCCACGCAGCAGGAAGCGGCCGAGCAGATCAAGTTCACGACCCAGT
TGCTGGTGGACTGGGTGGTTGTCGAGGCCGACAAGCTGCAAAAGCTGGTCGAGCAGACCGGCAAGAGCGTCAGCGAGTCT
CTGGACTCCTATTCCAGCTCCGGAGACTTTGACTTCGACGACGTCAAGATCGAGGATGCCCCCGAGGAAAAGGACAATGC
CGTCGGCGCCGAGGTCGAGGATGCGCCGGTCGTCAAGTTTCTGCACAAGATGCTGCTGGACGCCTTCAATATGCGCGCGT
CCGACTTGCACTTCGAGCCCTACGAACACAATTACCGCGTGCGCTTTCGTATCGACGGCGAGTTGCGCGAAATCGCCTCG
CCCCCTATCGCAATCAAGGACAAGCTGGCTTCCCGCATCAAGGTGATCTCGCGCCTGGACATCTCGGAGAAACGCGTTCC
GCAGGACGGTCGCATGAAGCTCAAGGTCGGCCCCGACCGCGTCATCGACTTTCGTGTCAGCACCTTGCCCACGCTTTTTG
GCGAGAAGATCGTGATCCGTATTCTGGACCCGAGCTCGGCCAAGATGGGCATCGACGCCCTGGGCTACGAACCCGAGGAA
AAGGAGCGCCTGCTCAAGGCCATCAACCGCCCCTACGGCATGATTCTGGTCACCGGCCCCACTGGCTCGGGCAAGACCGT
GTCGCTCTACACCTGCCTGAACCTGCTCAATCAGCCAGGCGTCAACATCGCCACCGCCGAAGACCCTTCCGAAATCAACA
TGCCTGGCGTCAACCAGGTCAATGTGAACGAGAAGGCCGGCCTGACCTTTGCCGCTGCGCTCAAATCCTTTCTGCGTCAG
GACCCGGACATCATCATGGTCGGTGAAATCCGTGACCTGGAAACCGCCGACATTTCGATCAAAGCCGCCCAGACCGGGCA
CCTGGTGCTGTCCACGCTGCACACCAATGATGCCCCGACCACACTGACGCGCATGCGCAATATGGGCATCGCCCCTTTCA
ATATTGCCTCCAGCGTGATTCTGATCACCGCCCAGCGTCTGGCACGACGCCTGTGTGCTCAGTGCAAGGAGCCCGCCGAC
ATACCGCACGACGCGCTGATCGAAGCCGGCTACGCTGAAGAGGAGCTCGACGGCAGCTGGGTCAGCTACAAGCCTGTGGG
TTGCCCTGCCTGCAACAACGGCTACAAGGGACGTGTCGGCATCTATCAGGTGATGCCCATCAGCGAGGAGATTCAGCGCA
TCATCCTGCGTGACGGCAGTGCGCTGGAAATTGCCGCACAGGCCAAGGCCGAAGGAGTACGCTCTCTGCGCGGCTCGGGC
CTGCATAAAGTCAAGCTGGGTCTGACCTCACTCGAAGAAGTGCTGGCGGTCACCAACGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.609

99.138

0.541
  pilB Acinetobacter baumannii D1279779

55.379

97.759

0.541
  pilF Neisseria gonorrhoeae MS11

52.827

97.586

0.516
  pilB Legionella pneumophila strain ERS1305867

50.883

97.586

0.497
  pilB Vibrio cholerae strain A1552

47.08

94.483

0.445
  pilB Vibrio campbellii strain DS40M4

46.286

90.517

0.419
  pilB Vibrio parahaemolyticus RIMD 2210633

46.008

90.69

0.417
  pilF Thermus thermophilus HB27

38.021

99.31

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.421

98.276

0.378

Multiple sequence alignment