Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACJMQR_RS19215 Genome accession   NZ_CP174465
Coordinates   3991552..3992769 (+) Length   405 a.a.
NCBI ID   WP_405118998.1    Uniprot ID   -
Organism   Pseudomonas leptonychotis strain TMP19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3986552..3997769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJMQR_RS19200 (ACJMQR_19200) - 3987399..3989099 (-) 1701 WP_405118995.1 Wzy polymerase domain-containing protein -
  ACJMQR_RS19205 (ACJMQR_19205) - 3989157..3989624 (-) 468 WP_405118996.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACJMQR_RS19210 (ACJMQR_19210) pilB 3989845..3991548 (+) 1704 WP_405118997.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJMQR_RS19215 (ACJMQR_19215) pilC 3991552..3992769 (+) 1218 WP_405118998.1 type II secretion system F family protein Machinery gene
  ACJMQR_RS19220 (ACJMQR_19220) pilD 3992773..3993645 (+) 873 WP_405118999.1 prepilin peptidase Machinery gene
  ACJMQR_RS19225 (ACJMQR_19225) coaE 3993807..3994421 (+) 615 WP_405119000.1 dephospho-CoA kinase -
  ACJMQR_RS19230 (ACJMQR_19230) yacG 3994418..3994618 (+) 201 WP_405119001.1 DNA gyrase inhibitor YacG -
  ACJMQR_RS19235 (ACJMQR_19235) - 3994663..3994878 (-) 216 WP_405119002.1 hypothetical protein -
  ACJMQR_RS19240 (ACJMQR_19240) - 3994983..3995672 (-) 690 WP_405119003.1 energy-coupling factor ABC transporter permease -
  ACJMQR_RS19245 (ACJMQR_19245) - 3995817..3996446 (+) 630 WP_405119004.1 DUF1780 domain-containing protein -
  ACJMQR_RS19250 (ACJMQR_19250) - 3996443..3996937 (+) 495 WP_405119005.1 MOSC domain-containing protein -
  ACJMQR_RS19255 (ACJMQR_19255) - 3997016..3997189 (+) 174 WP_136663137.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43891.54 Da        Isoelectric Point: 9.9374

>NTDB_id=1077173 ACJMQR_RS19215 WP_405118998.1 3991552..3992769(+) (pilC) [Pseudomonas leptonychotis strain TMP19]
MAVKALKTSTFAWEGTNKQGARIKGEVSGQNPALVKAQLRKQGINPTKVRKKSASLFSAGKKIKPMDIALFARQMATMMK
AGVPLLQSFDIIGEGLDNPNMRKLVDDIKQHVAAGNSFAASLRTRPEYFDDLFCNLVDAGEQAGALENLLDRVATYKEKT
EALKAKIKKAMNYPIAVVLVALIVSAILLIKVVPQFESVFAGFGAELPAFTQFVIGISQALQEWWFIVLIGMFATFFALG
ETKKRSEKFRNWIDRTALKAPIVGDIIYKSSVARYARTLATTFAAGVPLVEALDSVAGATGNIVFKNAVNKIKQDVSAGM
QLNFSMRSTNVFPSMAIQMTAIGEESGALDMMLDKVASYYEAEVDNAVDGLTALMEPLIMSVLGVLVGGLIIAMYLPIFQ
LGAVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1077173 ACJMQR_RS19215 WP_405118998.1 3991552..3992769(+) (pilC) [Pseudomonas leptonychotis strain TMP19]
ATGGCCGTTAAAGCACTAAAAACCAGCACTTTTGCTTGGGAAGGTACCAACAAGCAAGGCGCCAGAATAAAAGGTGAGGT
CAGCGGCCAGAACCCAGCACTCGTGAAGGCGCAGCTGCGCAAGCAGGGCATCAACCCGACCAAGGTTCGCAAAAAATCCG
CGTCACTATTCAGCGCTGGGAAAAAAATCAAACCAATGGACATCGCGCTGTTTGCACGGCAGATGGCAACCATGATGAAA
GCTGGCGTACCATTGCTACAGTCTTTTGACATCATTGGCGAGGGCCTAGACAACCCAAATATGCGTAAGCTGGTTGACGA
TATCAAACAACATGTTGCAGCCGGCAACAGCTTTGCTGCGTCTCTGCGCACTCGCCCAGAATACTTTGATGACCTGTTTT
GCAATCTGGTAGATGCAGGGGAACAAGCTGGTGCGCTTGAAAACCTGCTTGACCGGGTAGCCACTTATAAAGAAAAGACC
GAAGCACTTAAAGCCAAAATCAAAAAAGCAATGAACTACCCGATTGCAGTAGTTCTAGTCGCACTGATTGTTTCAGCCAT
CCTGCTGATAAAAGTAGTACCCCAATTCGAGTCTGTATTTGCTGGCTTTGGCGCAGAACTCCCCGCGTTTACTCAATTTG
TTATAGGTATCTCACAAGCCCTTCAAGAGTGGTGGTTTATAGTTCTGATAGGTATGTTTGCCACTTTTTTTGCACTGGGG
GAAACAAAAAAACGATCTGAGAAATTCAGAAATTGGATTGACCGGACCGCACTCAAAGCACCAATCGTTGGCGATATAAT
CTACAAATCATCCGTAGCTCGCTATGCCCGGACCCTGGCCACCACATTTGCCGCAGGCGTACCCCTCGTGGAAGCTTTAG
ACTCTGTAGCCGGCGCAACCGGTAATATCGTATTCAAGAATGCCGTGAATAAAATTAAGCAAGATGTCAGTGCTGGCATG
CAGCTTAATTTTTCCATGCGCAGTACTAACGTGTTCCCCTCAATGGCCATCCAAATGACTGCCATCGGTGAAGAGTCAGG
GGCGCTGGATATGATGCTGGATAAAGTAGCAAGCTATTACGAGGCCGAAGTTGACAATGCCGTTGACGGTTTGACTGCCC
TGATGGAACCACTGATCATGTCGGTACTCGGCGTACTGGTCGGCGGCTTGATCATTGCTATGTACCTGCCAATCTTCCAA
CTTGGCGCAGTTGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

80

100

0.8
  pilC Acinetobacter baumannii D1279779

60.539

100

0.61
  pilC Acinetobacter baylyi ADP1

59.95

98.025

0.588
  pilC Legionella pneumophila strain ERS1305867

53.652

98.025

0.526
  pilG Neisseria meningitidis 44/76-A

43.781

99.259

0.435
  pilG Neisseria gonorrhoeae MS11

43.781

99.259

0.435
  pilC Vibrio cholerae strain A1552

42.716

100

0.427
  pilC Vibrio campbellii strain DS40M4

40.394

100

0.405
  pilC Thermus thermophilus HB27

36.5

98.765

0.36

Multiple sequence alignment