Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACI3EV_RS05010 Genome accession   NZ_CP173677
Coordinates   1046472..1047677 (-) Length   401 a.a.
NCBI ID   WP_329770235.1    Uniprot ID   -
Organism   Brachymonas sp. G13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1041472..1052677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3EV_RS04985 (ACI3EV_04985) - 1042733..1042972 (-) 240 WP_329770230.1 DNA gyrase inhibitor YacG -
  ACI3EV_RS04990 (ACI3EV_04990) zapD 1042985..1043740 (-) 756 WP_337690600.1 cell division protein ZapD -
  ACI3EV_RS04995 (ACI3EV_04995) coaE 1043737..1044372 (-) 636 WP_337690599.1 dephospho-CoA kinase -
  ACI3EV_RS05000 (ACI3EV_05000) - 1044450..1045295 (-) 846 WP_329770233.1 A24 family peptidase -
  ACI3EV_RS05005 (ACI3EV_05005) - 1045292..1046488 (-) 1197 WP_329770234.1 type II secretion system F family protein -
  ACI3EV_RS05010 (ACI3EV_05010) pilF 1046472..1047677 (-) 1206 WP_329770235.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  ACI3EV_RS05015 (ACI3EV_05015) - 1047758..1048456 (+) 699 WP_329770236.1 histidine phosphatase family protein -
  ACI3EV_RS05020 (ACI3EV_05020) - 1048456..1050003 (+) 1548 WP_405198045.1 ABC transporter substrate-binding protein -
  ACI3EV_RS05025 (ACI3EV_05025) - 1050019..1050804 (-) 786 WP_329770237.1 ATP-binding cassette domain-containing protein -
  ACI3EV_RS05030 (ACI3EV_05030) - 1050801..1051781 (-) 981 WP_337690598.1 ABC transporter ATP-binding protein -
  ACI3EV_RS05035 (ACI3EV_05035) - 1051781..1052662 (-) 882 WP_337690597.1 ABC transporter permease -

Sequence

Protein


Download         Length: 401 a.a.        Molecular weight: 43785.39 Da        Isoelectric Point: 7.7660

>NTDB_id=1073652 ACI3EV_RS05010 WP_329770235.1 1046472..1047677(-) (pilF) [Brachymonas sp. G13]
MYPPAAAPPLPSADDSSAVSWLQALLAAAWARGASDLHLEPYEHSYRVRLRIDGQLQPVAAPPASLKERIASRIKVLARL
DIAEKRLPQDGQFRLELPAGQTGDLRVSTLPTLFGEKIVIRLLPTDNAQVQLDALGLEPLQLQQLRDAIQRPQGMVLVTG
PTGSGKTITLYSCLQALNRPDVNIASVEDPCEIRLEGINQVHLHEKAGLDYASVLRAFLRQDPDILMVGEIRDLATADTA
LKAAQTGHLVLSTLHTLDAPSALTRLQQMGVPPYQLAASISLVVAQRLVRQLCRHCRQPQPLHASQWLAAGLPETELASR
PQAWQPVGCAHCHQGYRGRTGIFQLMPISSELQDAMLQQAASHQLQGLARQLGMRSLRQAGLRKVLQGETSLQEVLHATT
D

Nucleotide


Download         Length: 1206 bp        

>NTDB_id=1073652 ACI3EV_RS05010 WP_329770235.1 1046472..1047677(-) (pilF) [Brachymonas sp. G13]
ATGTACCCACCTGCCGCCGCCCCACCCCTACCCTCTGCCGACGACAGCAGCGCCGTGTCCTGGTTGCAGGCGCTGCTGGC
TGCGGCCTGGGCACGCGGCGCATCCGACCTGCATCTGGAACCCTACGAACACAGCTACCGGGTACGGCTGCGCATCGACG
GCCAGCTGCAGCCGGTGGCAGCTCCGCCAGCCAGCCTCAAGGAACGCATCGCCAGCCGCATCAAGGTGCTGGCGCGACTC
GATATCGCCGAAAAACGCCTGCCGCAGGACGGCCAGTTCCGGCTGGAATTGCCAGCCGGGCAGACGGGTGATCTGCGCGT
CAGCACCCTGCCCACGCTGTTTGGCGAAAAAATCGTGATTCGCCTGCTGCCCACCGACAACGCCCAGGTGCAGCTCGACG
CCCTCGGGCTGGAGCCGCTGCAGCTGCAACAGTTGCGCGATGCCATCCAGAGGCCACAAGGCATGGTGCTGGTGACCGGG
CCGACCGGCTCCGGCAAGACCATCACGCTGTACAGCTGCCTGCAAGCGCTGAACCGTCCCGATGTGAATATCGCCAGTGT
CGAAGACCCGTGCGAAATCCGGCTGGAAGGCATCAACCAGGTGCATCTGCATGAAAAAGCCGGGCTGGATTACGCCAGCG
TGCTGCGCGCCTTTCTGCGCCAGGATCCGGACATCCTGATGGTCGGTGAAATCCGCGATCTGGCTACGGCCGACACCGCC
CTCAAGGCAGCGCAGACCGGGCATCTGGTGCTGTCCACGCTGCACACGCTCGATGCGCCCTCGGCGCTGACGCGGCTGCA
GCAAATGGGGGTGCCGCCCTACCAGCTGGCCGCCAGCATCAGCCTGGTCGTGGCGCAGCGGCTGGTGCGCCAGCTGTGCC
GGCACTGCCGCCAGCCGCAGCCACTGCATGCCTCGCAATGGCTGGCTGCCGGCCTGCCGGAGACGGAGCTCGCCTCGCGC
CCGCAGGCATGGCAGCCGGTCGGTTGCGCCCATTGTCATCAGGGCTACCGAGGCCGCACCGGCATCTTCCAGCTGATGCC
GATCAGCAGCGAGCTGCAGGACGCCATGTTGCAGCAGGCCGCCAGCCACCAGCTGCAAGGACTGGCACGCCAGCTTGGCA
TGCGCAGCCTGCGCCAGGCCGGCTTGCGCAAGGTGCTGCAGGGCGAGACATCCTTGCAAGAGGTATTGCATGCCACCACC
GACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(25-291)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

51.786

97.756

0.506
  pilB Vibrio campbellii strain DS40M4

51.554

96.259

0.496
  pilB Acinetobacter baumannii D1279779

50.255

97.756

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

51.042

95.761

0.489
  pilB Vibrio cholerae strain A1552

50.646

96.509

0.489
  pilB Acinetobacter baylyi ADP1

50.781

95.761

0.486
  pilB Legionella pneumophila strain ERS1305867

49.614

97.007

0.481
  pilF Thermus thermophilus HB27

43.367

97.756

0.424
  pilB Haemophilus influenzae Rd KW20

43.717

95.262

0.416
  pilB Glaesserella parasuis strain SC1401

43.767

94.015

0.411
  pilB Haemophilus influenzae 86-028NP

43.194

95.262

0.411
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.837

97.756

0.409
  pilB/pilB1 Synechocystis sp. PCC 6803

40.594

100

0.409
  ctsE Campylobacter jejuni subsp. jejuni 81-176

40.206

96.758

0.389

Multiple sequence alignment