Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACI537_RS03700 Genome accession   NZ_CP173426
Coordinates   862219..863955 (-) Length   578 a.a.
NCBI ID   WP_404983616.1    Uniprot ID   -
Organism   Cobetia marina strain KMM 1449     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 857219..868955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI537_RS03675 (ACI537_03675) - 857808..858761 (+) 954 WP_404983613.1 Nudix family hydrolase -
  ACI537_RS03680 (ACI537_03680) yacG 858890..859135 (-) 246 WP_141392184.1 DNA gyrase inhibitor YacG -
  ACI537_RS03685 (ACI537_03685) coaE 859128..859775 (-) 648 WP_404983614.1 dephospho-CoA kinase -
  ACI537_RS03690 (ACI537_03690) pilD 859775..860635 (-) 861 WP_084209205.1 A24 family peptidase Machinery gene
  ACI537_RS03695 (ACI537_03695) pilC 860735..861985 (-) 1251 WP_404983615.1 type II secretion system F family protein Machinery gene
  ACI537_RS03700 (ACI537_03700) pilB 862219..863955 (-) 1737 WP_404983616.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACI537_RS03705 (ACI537_03705) - 864716..865159 (+) 444 WP_136938320.1 pilin -
  ACI537_RS03710 (ACI537_03710) - 865231..866958 (+) 1728 WP_404983617.1 hypothetical protein -
  ACI537_RS03715 (ACI537_03715) - 866990..867292 (-) 303 WP_404983618.1 hypothetical protein -
  ACI537_RS03720 (ACI537_03720) - 867417..868697 (-) 1281 WP_404983619.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63516.56 Da        Isoelectric Point: 4.8035

>NTDB_id=1072229 ACI537_RS03700 WP_404983616.1 862219..863955(-) (pilB) [Cobetia marina strain KMM 1449]
MPQSDQTLKGFAARLVESGLLEPQAALLAEQEANEAGESLLLHVITHGLASAREATLVAAWEYGLPYVDLDAIRIDSLPS
IDELPEKVLRKLGVLPLYRREHRLTVGVPSPSTLTHLDELQFATGLSIESVLVAADQLAPALEQYLEAGEESAMDMLSED
GEALDGLEFEGEEDNNHDMAITANSDDAPVVRFVHKVLLDAIKRGASDIHFEPYESTYRIRFRIDGMLVENTRPPFNMRN
RISARLKVMSRMDISERRLPQDGAIKLRLSKSRSIDFRVNTLPTVYGEKLVLRILDPSSAKMGIEALGFEPEQRAMFEKT
LAEPQGMILVTGPTGSGKTVTLYTGLNILNTPERNISTAEDPVELKVEGVNQVNVLPKIGLDFASALRAFLRQDPDVVMV
GEIRDKETAEIAVKAAQTGHLVLSTLHTNSAPETLTRLSNMGIAPFNIASSVSLIIAQRLARRLCPHCRQPAEIPQEAMA
SMGFSAENGFSAEDLDNATIYRPTGCSQCTLGYKGRVGIYEVMPISDEMSRLIMREGTAIELADQARKEGLSDLRRSGLR
KVMAGMTSLEEVNRITKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1072229 ACI537_RS03700 WP_404983616.1 862219..863955(-) (pilB) [Cobetia marina strain KMM 1449]
ATGCCCCAGAGTGATCAGACATTGAAGGGATTTGCCGCTCGCCTGGTGGAATCAGGCTTGCTGGAGCCTCAGGCTGCCTT
GCTGGCTGAGCAGGAGGCCAATGAAGCTGGCGAGAGTCTGCTGCTGCATGTCATCACTCATGGCTTGGCTTCGGCACGTG
AGGCCACCTTGGTGGCAGCATGGGAATACGGGCTGCCCTATGTGGATCTCGATGCAATACGTATCGATTCACTCCCGAGC
ATTGATGAGCTGCCAGAGAAGGTACTGCGAAAGCTGGGCGTACTGCCGCTCTATCGACGCGAACATCGTTTGACCGTCGG
CGTGCCCTCGCCATCGACACTGACTCACCTTGATGAATTGCAGTTTGCCACCGGCCTGTCGATTGAAAGCGTACTGGTCG
CAGCCGATCAACTCGCACCTGCGCTAGAGCAATATCTGGAAGCCGGCGAAGAAAGCGCCATGGACATGCTGAGCGAAGAT
GGCGAGGCTCTAGATGGACTCGAATTCGAAGGCGAAGAAGACAACAACCATGATATGGCCATCACGGCCAATAGTGATGA
TGCGCCAGTCGTGCGATTCGTTCACAAGGTACTGTTGGATGCCATCAAACGTGGCGCCTCGGACATTCACTTTGAACCTT
ATGAATCCACGTACCGGATTCGCTTTCGTATCGATGGCATGCTGGTCGAGAATACTCGTCCGCCCTTCAACATGCGCAAC
CGTATCTCGGCGCGCTTGAAGGTGATGTCGCGCATGGATATCTCCGAGCGACGTCTGCCTCAAGATGGCGCCATCAAACT
GCGCCTGTCCAAGTCACGCTCCATCGATTTTCGTGTCAACACCTTACCAACCGTTTACGGGGAGAAGTTGGTACTGCGTA
TTCTTGACCCCAGTTCCGCCAAGATGGGGATCGAGGCGCTGGGCTTCGAGCCGGAACAGCGTGCCATGTTCGAGAAGACA
CTTGCCGAGCCTCAGGGCATGATTCTGGTCACCGGCCCTACCGGTAGCGGCAAGACCGTCACCCTGTATACCGGCTTGAA
CATCCTCAACACGCCTGAGCGCAATATCTCGACCGCGGAAGACCCGGTCGAGCTCAAGGTGGAGGGTGTCAATCAGGTCA
ACGTACTACCCAAGATAGGACTCGACTTCGCCTCGGCACTGCGCGCTTTCCTGCGCCAAGATCCCGACGTGGTGATGGTC
GGGGAGATTCGGGACAAGGAAACCGCGGAAATCGCCGTCAAGGCCGCGCAGACAGGCCACCTGGTGCTCTCGACCCTGCA
TACCAATTCTGCCCCTGAAACGTTGACGCGCCTCAGCAACATGGGAATTGCGCCCTTCAACATCGCAAGCTCGGTTTCCT
TGATCATCGCGCAACGCCTGGCGCGGCGCCTGTGTCCCCACTGTCGCCAACCTGCCGAGATCCCCCAGGAAGCCATGGCC
TCGATGGGCTTCAGCGCAGAAAATGGCTTCAGTGCGGAAGATCTGGACAATGCCACCATCTATCGCCCGACGGGTTGCAG
CCAATGCACGCTGGGCTACAAGGGACGCGTCGGCATCTATGAAGTGATGCCCATCTCTGATGAGATGAGCCGCTTGATCA
TGCGGGAAGGCACCGCGATAGAACTGGCGGACCAGGCGCGCAAGGAAGGGTTATCGGATCTGCGCCGCAGCGGCCTGCGC
AAGGTCATGGCGGGCATGACCTCGCTGGAAGAGGTCAACCGTATCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

52.797

98.962

0.522
  pilB Acinetobacter baumannii D1279779

52.265

99.308

0.519
  pilB Acinetobacter baylyi ADP1

51.923

98.962

0.514
  pilB Vibrio campbellii strain DS40M4

47.826

99.481

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

48.148

98.097

0.472
  pilB Vibrio cholerae strain A1552

49.094

95.502

0.469
  pilF Neisseria gonorrhoeae MS11

48.561

96.194

0.467
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.046

97.924

0.382
  pilB/pilB1 Synechocystis sp. PCC 6803

42.065

90.484

0.381
  pilF Thermus thermophilus HB27

38.053

97.751

0.372

Multiple sequence alignment