Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACI3L7_RS00845 Genome accession   NZ_CP173387
Coordinates   205338..207023 (+) Length   561 a.a.
NCBI ID   WP_404970138.1    Uniprot ID   -
Organism   Vibrio campbellii strain LB135     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 200338..212023
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3L7_RS00825 (ACI3L7_00825) pdhR 201905..202672 (-) 768 WP_005428674.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  ACI3L7_RS00830 (ACI3L7_00830) ampD 203077..203628 (-) 552 WP_404970137.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACI3L7_RS00835 (ACI3L7_00835) nadC 203721..204608 (+) 888 WP_012128875.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACI3L7_RS00840 (ACI3L7_00840) pilA 204874..205338 (+) 465 WP_051118346.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACI3L7_RS00845 (ACI3L7_00845) pilB 205338..207023 (+) 1686 WP_404970138.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACI3L7_RS00850 (ACI3L7_00850) pilC 207058..208281 (+) 1224 WP_050905227.1 type II secretion system F family protein Machinery gene
  ACI3L7_RS00855 (ACI3L7_00855) pilD 208354..209223 (+) 870 WP_012128879.1 A24 family peptidase Machinery gene
  ACI3L7_RS00860 (ACI3L7_00860) coaE 209224..209838 (+) 615 WP_012128880.1 dephospho-CoA kinase -
  ACI3L7_RS00865 (ACI3L7_00865) zapD 209866..210606 (+) 741 WP_012128881.1 cell division protein ZapD -
  ACI3L7_RS00870 (ACI3L7_00870) yacG 210669..210863 (+) 195 WP_404970139.1 DNA gyrase inhibitor YacG -
  ACI3L7_RS00875 (ACI3L7_00875) rplS 211379..211732 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62110.92 Da        Isoelectric Point: 5.1898

>NTDB_id=1071900 ACI3L7_RS00845 WP_404970138.1 205338..207023(+) (pilB) [Vibrio campbellii strain LB135]
MLTNLSTVLRQAGILTFSQEESLLEQAKASGISMPEALLSSGFFQSHELTEHLSSIFGLSCTSLSQYEYSSLCQKLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRSLGQEKSGLKEINQDELAG
LVDIGEDEIENIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILIEIQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSAALDINKLGYSDSQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPYCRQPQEPNSQLQHIGITSTEQ
IFQANPDGCNECTHGYSGRTGIYEVMKFDESLSEALIKGVSVHELEKLAIANGMQTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1071900 ACI3L7_RS00845 WP_404970138.1 205338..207023(+) (pilB) [Vibrio campbellii strain LB135]
ATGCTAACCAATCTCTCAACGGTTCTTCGTCAAGCGGGAATACTGACTTTTTCCCAAGAAGAATCGTTACTTGAGCAAGC
AAAAGCTTCTGGCATCTCGATGCCGGAGGCTTTACTCAGTTCTGGCTTTTTTCAATCTCACGAATTGACTGAGCACCTGA
GTTCTATCTTTGGGCTTTCCTGCACATCACTCAGTCAGTATGAATACTCGTCGCTGTGCCAAAAGCTTGGCTTGCGCGAT
CTGATCACTCGACATAATGCCTTGCCACTCAATCGCACATCTTCGACATTAATACTTGCGGTTGCCGACCCAACAAACCT
TCAAGCCGAAGATGACTTTCGTTTTGCGACAGGCTTGCAAGTCGAGCTTGTCCTCGCTGACTTTCGTGAGCTAACAGCAG
CCATTCGACGTTTATATGGCCGATCACTTGGGCAAGAAAAATCCGGTCTTAAAGAAATCAACCAAGATGAACTGGCGGGT
TTGGTTGATATTGGTGAGGATGAAATTGAGAACATCGAAGATCTCAGCCAAGATGAATCGCCGGTCAGTCGCTATATCAA
CCAAATTTTACTCGATGCTGTCCGTAAAGGGGCTTCCGATATTCACTTCGAGCCCTATGAAAACATGTACCGCGTGCGCT
TGCGTTGCGATGGCATCTTAATTGAAATTCAGCAGCCTCCGAGTCATTTAAGTCGTCGCCTCTCCGCTCGCATAAAAATC
TTATCTAAGCTTGATATCGCCGAGCGCCGTTTACCACAGGATGGGCGAATCAAGCTCAAATTAAACCAAGACACTGCAAT
TGATATGCGTGTCTCCACACTACCAACTCTTTTTGGAGAAAAGATTGTTCTGCGTTTGCTCGACAGCAGTTCTGCAGCAT
TAGATATCAATAAGCTCGGTTATAGCGACTCACAGAAACAACTTTATTTGGATGCCCTGCGCCGTCCGCAAGGTATGATC
CTGATGACAGGCCCAACAGGTAGCGGTAAAACGGTCTCGCTCTATACTGGATTGAGTATTCTTAATAAGCCAGAGATCAA
TATCTCAACCGCTGAAGATCCCGTTGAGATTAACCTGCCCGGCATTAACCAAGTTCAAGTTCAGCCAAAGATCGGCTTTG
GTTTTGCAGAGGCGCTTCGCTCTTTCTTACGTCAAGATCCTGATGTGGTTATGGTGGGCGAGATCCGAGATCTCGACACA
GCAGAAATCGCGATCAAAGCATCACAGACTGGCCACTTAGTTCTCTCCACTTTGCACACGAACTCGGCTGCCGAGACTGT
CATTCGCTTGTCTAATATGGGCGTAGAAAGCTTTAACCTCGCCTCCTCTCTCAGTTTGATCATCGCCCAGCGTTTGGCTA
GAAAACTGTGTCCATATTGTAGACAACCTCAAGAGCCAAACAGCCAACTTCAACATATTGGAATCACCAGCACCGAACAG
ATCTTTCAAGCTAACCCCGACGGTTGTAACGAGTGTACTCATGGTTATTCAGGGCGAACGGGCATTTACGAAGTGATGAA
GTTTGATGAATCCCTTTCAGAAGCACTCATCAAAGGCGTCTCGGTACACGAATTAGAAAAGCTCGCCATTGCCAATGGTA
TGCAAACCTTACAAATGTCTGGCATCGAAAAACTCAAACAAGGCATTACCAGCTTCAGCGAATTGCAGCGCGTTCTCTAC
TTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

98.217

100

0.982
  pilB Vibrio parahaemolyticus RIMD 2210633

90.553

100

0.906
  pilB Vibrio cholerae strain A1552

73.488

100

0.736
  pilB Acinetobacter baumannii D1279779

51.445

92.513

0.476
  pilB Acinetobacter baylyi ADP1

47.849

99.465

0.476
  pilB Legionella pneumophila strain ERS1305867

48.339

96.613

0.467
  pilF Neisseria gonorrhoeae MS11

43.44

100

0.437
  pilB/pilB1 Synechocystis sp. PCC 6803

36.7

100

0.389
  pilF Thermus thermophilus HB27

36.833

100

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.559

99.465

0.364

Multiple sequence alignment