Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACH784_RS14715 Genome accession   NZ_CP173161
Coordinates   3248836..3250071 (+) Length   411 a.a.
NCBI ID   WP_404391440.1    Uniprot ID   -
Organism   Pseudoalteromonas phenolica strain a1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3243836..3255071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH784_RS14695 (ACH784_14695) ampE 3245290..3246141 (-) 852 WP_404391432.1 beta-lactamase regulator AmpE -
  ACH784_RS14700 (ACH784_14700) ampD 3246149..3246706 (-) 558 WP_404391434.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACH784_RS14705 (ACH784_14705) nadC 3246847..3247713 (+) 867 WP_404391436.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACH784_RS14710 (ACH784_14710) - 3248284..3248784 (+) 501 WP_404391438.1 pilin -
  ACH784_RS14715 (ACH784_14715) pilC 3248836..3250071 (+) 1236 WP_404391440.1 type II secretion system F family protein Machinery gene
  ACH784_RS14720 (ACH784_14720) pilD 3250108..3250998 (+) 891 WP_404391442.1 prepilin peptidase Machinery gene
  ACH784_RS14725 (ACH784_14725) coaE 3251027..3251635 (+) 609 WP_404391444.1 dephospho-CoA kinase -
  ACH784_RS14730 (ACH784_14730) pilB 3251772..3253454 (+) 1683 WP_404391446.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACH784_RS14735 (ACH784_14735) yacG 3253556..3253795 (+) 240 WP_404395284.1 DNA gyrase inhibitor YacG -
  ACH784_RS14740 (ACH784_14740) - 3253900..3254340 (-) 441 WP_404391448.1 Spy/CpxP family protein refolding chaperone -

Sequence

Protein


Download         Length: 411 a.a.        Molecular weight: 44667.55 Da        Isoelectric Point: 9.7447

>NTDB_id=1070342 ACH784_RS14715 WP_404391440.1 3248836..3250071(+) (pilC) [Pseudoalteromonas phenolica strain a1]
MKKDKDSADKLDTFQWTGLSARGKKLEGDMTGTSIALVKAQLRKQGITPSKVKRKPKPLFGIGAGGQKITAKDIAIFTRQ
IATMLTAGVPLIQSLEMISSGAKNKSLSKLIGSIADEVKAGQPLAKSLRAYPRYFDELYCDLIHSGEQSGALDKIFDRVA
VYKEKAEALKSKIKKAMFYPIAVVVVALIVTSILLIFVVPQFQDIFSSFGAELPAFTLFVIGISEFMQKSWWIFLLVIGI
SIYSYKEALIRSSAVRHFNDRLVLQFPVVGMILHKAAVARYARTLSTTFAAGVPLVDALDSAAGASGNVIYKNAIVEIKA
EVSSGNQMNWAMRNSKLFPDMVIQMVSIGEESGSLDGMLEKVANIYEQEVDDAVDGLSSLLEPLIMAVLAVLVGGLIIAM
YLPIFQLGSVV

Nucleotide


Download         Length: 1236 bp        

>NTDB_id=1070342 ACH784_RS14715 WP_404391440.1 3248836..3250071(+) (pilC) [Pseudoalteromonas phenolica strain a1]
ATGAAAAAAGACAAAGATTCTGCCGATAAACTCGATACCTTTCAGTGGACTGGTTTAAGCGCTCGAGGCAAAAAGCTCGA
AGGCGATATGACTGGTACCAGCATTGCCCTTGTTAAAGCCCAACTTCGTAAACAAGGCATTACTCCTTCTAAAGTAAAAC
GCAAACCTAAACCCTTATTTGGTATAGGTGCAGGTGGTCAAAAGATTACCGCCAAAGATATTGCGATTTTCACTCGACAA
ATTGCCACCATGTTGACTGCGGGCGTACCGCTTATTCAGTCTTTAGAAATGATTTCATCTGGGGCAAAAAACAAAAGTTT
AAGTAAACTCATTGGCAGCATTGCCGATGAAGTTAAAGCCGGCCAACCACTGGCAAAATCACTACGTGCTTACCCTCGAT
ATTTCGACGAACTCTATTGCGACCTGATCCACTCAGGGGAACAGTCTGGTGCTCTTGATAAAATTTTTGACCGTGTTGCC
GTTTATAAAGAAAAAGCCGAGGCGCTAAAATCAAAAATCAAAAAGGCCATGTTTTACCCTATTGCGGTAGTCGTTGTAGC
TTTAATTGTGACCTCTATTCTACTTATTTTCGTGGTACCGCAATTCCAAGATATCTTCAGCAGCTTTGGCGCTGAATTAC
CCGCATTTACTTTATTTGTAATCGGTATCTCAGAGTTTATGCAAAAATCTTGGTGGATATTCTTATTAGTCATAGGTATA
AGTATTTATTCATATAAAGAAGCGTTAATTCGATCATCTGCGGTGCGTCACTTTAACGACCGCTTAGTTTTGCAATTCCC
TGTCGTTGGCATGATCTTACATAAAGCAGCTGTAGCACGTTATGCTCGTACACTGTCGACTACATTTGCAGCGGGTGTAC
CGCTGGTAGACGCGCTCGACTCTGCTGCGGGCGCATCAGGCAATGTCATCTATAAAAATGCCATTGTCGAAATCAAAGCT
GAGGTAAGCTCTGGTAACCAAATGAACTGGGCGATGCGTAACTCTAAGCTGTTTCCTGATATGGTGATCCAAATGGTCTC
TATTGGTGAAGAGTCTGGTTCACTCGACGGCATGCTTGAAAAAGTCGCCAATATTTACGAACAAGAAGTAGATGATGCCG
TAGACGGTCTATCTAGTCTGTTAGAACCTTTAATTATGGCGGTGTTAGCTGTACTTGTAGGTGGCCTTATTATTGCCATG
TACTTACCTATCTTCCAGCTGGGCTCTGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(282-403)

Type II secretion system (T2SS), protein F (T2SSF)

(77-200)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.443

97.81

0.601
  pilC Legionella pneumophila strain ERS1305867

54.902

99.27

0.545
  pilC Acinetobacter baylyi ADP1

55.86

97.567

0.545
  pilC Acinetobacter baumannii D1279779

53.96

98.297

0.53
  pilC Vibrio campbellii strain DS40M4

44.039

100

0.44
  pilC Vibrio cholerae strain A1552

43.284

97.81

0.423
  pilG Neisseria meningitidis 44/76-A

42.092

95.377

0.401
  pilG Neisseria gonorrhoeae MS11

41.837

95.377

0.399

Multiple sequence alignment