Detailed information NTG1070343

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACH784_RS14720
• Genome accession: NZ_CP173161
• Coordinates: 3250108..3250998 (+)
• Length: 296 a.a.
• NCBI ID: WP_404391442.1
• Uniprot ID: -
• Organism: Pseudoalteromonas phenolica strain a1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3245108..3255998

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACH784_RS14695 (ACH784_14695)	ampE	3245290..3246141 (-)	852	WP_404391432.1	beta-lactamase regulator AmpE	-
ACH784_RS14700 (ACH784_14700)	ampD	3246149..3246706 (-)	558	WP_404391434.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
ACH784_RS14705 (ACH784_14705)	nadC	3246847..3247713 (+)	867	WP_404391436.1	carboxylating nicotinate-nucleotide diphosphorylase	-
ACH784_RS14710 (ACH784_14710)	-	3248284..3248784 (+)	501	WP_404391438.1	pilin	-
ACH784_RS14715 (ACH784_14715)	pilC	3248836..3250071 (+)	1236	WP_404391440.1	type II secretion system F family protein	Machinery gene
ACH784_RS14720 (ACH784_14720)	pilD	3250108..3250998 (+)	891	WP_404391442.1	prepilin peptidase	Machinery gene
ACH784_RS14725 (ACH784_14725)	coaE	3251027..3251635 (+)	609	WP_404391444.1	dephospho-CoA kinase	-
ACH784_RS14730 (ACH784_14730)	pilB	3251772..3253454 (+)	1683	WP_404391446.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACH784_RS14735 (ACH784_14735)	yacG	3253556..3253795 (+)	240	WP_404395284.1	DNA gyrase inhibitor YacG	-
ACH784_RS14740 (ACH784_14740)	-	3253900..3254340 (-)	441	WP_404391448.1	Spy/CpxP family protein refolding chaperone	-
ACH784_RS14745 (ACH784_14745)	-	3254490..3255173 (+)	684	WP_404391450.1	response regulator	-

Sequence

Protein

• Length: 296 a.a.
• Molecular weight: 32736.94 Da
• Isoelectric Point: 7.6369

>NTDB_id=1070343 ACH784_RS14720 WP_404391442.1 3250108..3250998(+) (pilD) [Pseudoalteromonas phenolica strain a1]
MQEVIYLMQSQQWFFLLTVGLVSLCIGSFLNVVIYRLPIMMQRQWQQECRISLADELANSSKQTASSNDTFNLVKPNSTC
PHCKTPIKAWHNIPLLGWLMLKGKCTSCSAKISVRYPLVELGTALTSLWIALHFGATELGLLYIFITWVLVALIFIDIDH
MLLPDQLTLPLVWLALVASVMGYTIAPSDAIIGAAVGYLSLWTVFWAFKLLTGKEGMGYGDFKLLAIFGALLGWQAILTI
VLLSSVVGAIIGSIQLAVQGKEHSTPIPFGPYLAIAGWLVMLYGTNIQTWYFSLLG

Nucleotide

• Length: 891 bp

>NTDB_id=1070343 ACH784_RS14720 WP_404391442.1 3250108..3250998(+) (pilD) [Pseudoalteromonas phenolica strain a1]
ATGCAAGAAGTAATTTATTTGATGCAGAGCCAGCAATGGTTTTTTCTGTTAACCGTTGGCTTAGTCAGCCTGTGTATTGG
CAGCTTTCTAAATGTGGTGATTTATCGCCTACCTATCATGATGCAGCGTCAGTGGCAGCAAGAGTGCCGTATTTCACTGG
CCGATGAGTTGGCAAACTCGAGTAAACAAACAGCATCGAGCAACGACACTTTCAACCTAGTTAAGCCTAACTCGACCTGC
CCACACTGTAAAACCCCAATCAAAGCTTGGCATAATATTCCGTTATTGGGCTGGCTAATGCTAAAAGGCAAATGCACCAG
TTGCTCAGCAAAAATCTCTGTCCGCTATCCATTAGTTGAGCTAGGCACTGCCCTTACCTCGCTATGGATTGCACTGCACT
TTGGCGCAACTGAGCTTGGGTTACTGTATATTTTTATTACTTGGGTGTTAGTGGCGCTTATTTTTATTGATATCGACCAC
ATGCTATTGCCCGACCAACTTACGCTTCCTTTAGTTTGGCTGGCATTAGTTGCCAGTGTTATGGGTTATACCATTGCCCC
CAGCGACGCCATTATCGGGGCTGCGGTTGGTTACTTGAGTTTATGGACCGTATTTTGGGCATTCAAACTGCTGACAGGTA
AAGAGGGCATGGGCTATGGGGACTTTAAACTATTAGCCATTTTTGGTGCGCTATTAGGTTGGCAGGCTATTTTGACCATA
GTTTTATTATCGAGTGTTGTTGGCGCCATCATTGGCAGCATTCAATTAGCTGTGCAAGGTAAAGAGCATAGTACGCCTAT
TCCATTTGGCCCTTATTTAGCTATCGCCGGTTGGCTGGTAATGCTCTATGGTACAAATATTCAAACTTGGTACTTTTCTC
TTTTAGGCTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-134)

• Type IV leader peptidase family (Peptidase_A24) (145-252)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.901	95.27	0.514
pilD	Vibrio campbellii strain DS40M4	50.685	98.649	0.5
pilD	Acinetobacter nosocomialis M2	49.315	98.649	0.486
pilD	Acinetobacter baumannii D1279779	48.973	98.649	0.483
pilD	Neisseria gonorrhoeae MS11	41.697	91.554	0.382