Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACH784_RS14730 Genome accession   NZ_CP173161
Coordinates   3251772..3253454 (+) Length   560 a.a.
NCBI ID   WP_404391446.1    Uniprot ID   -
Organism   Pseudoalteromonas phenolica strain a1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3246772..3258454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH784_RS14705 (ACH784_14705) nadC 3246847..3247713 (+) 867 WP_404391436.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACH784_RS14710 (ACH784_14710) - 3248284..3248784 (+) 501 WP_404391438.1 pilin -
  ACH784_RS14715 (ACH784_14715) pilC 3248836..3250071 (+) 1236 WP_404391440.1 type II secretion system F family protein Machinery gene
  ACH784_RS14720 (ACH784_14720) pilD 3250108..3250998 (+) 891 WP_404391442.1 prepilin peptidase Machinery gene
  ACH784_RS14725 (ACH784_14725) coaE 3251027..3251635 (+) 609 WP_404391444.1 dephospho-CoA kinase -
  ACH784_RS14730 (ACH784_14730) pilB 3251772..3253454 (+) 1683 WP_404391446.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACH784_RS14735 (ACH784_14735) yacG 3253556..3253795 (+) 240 WP_404395284.1 DNA gyrase inhibitor YacG -
  ACH784_RS14740 (ACH784_14740) - 3253900..3254340 (-) 441 WP_404391448.1 Spy/CpxP family protein refolding chaperone -
  ACH784_RS14745 (ACH784_14745) - 3254490..3255173 (+) 684 WP_404391450.1 response regulator -
  ACH784_RS14750 (ACH784_14750) - 3255154..3256551 (+) 1398 WP_404391452.1 ATP-binding protein -
  ACH784_RS14755 (ACH784_14755) - 3256548..3257513 (+) 966 WP_404391454.1 alpha/beta fold hydrolase -
  ACH784_RS14760 (ACH784_14760) - 3257465..3258364 (-) 900 WP_404391456.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 62268.67 Da        Isoelectric Point: 5.7294

>NTDB_id=1070344 ACH784_RS14730 WP_404391446.1 3251772..3253454(+) (pilB) [Pseudoalteromonas phenolica strain a1]
MEHHSPLLRKFITLGRVTAEQLKLKQAEAQSTAELICLCSGMTGQELAEQCLDLFRVPMFDLSDFDVEQLPKELIKEKLI
RQHHLLPLVKKGRKLFIATSDPTDYGAFENFEFSTGLQCEIVVVDYKQLEQKIDQLFDANGGLSMSEDDFKEFSGMDVED
TQQQQDSNDEKDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGMLHEMASPPTSLSTRLSARIKVMSRL
DIAEKRKPQDGRIKLKISDRKSIDFRVSTLPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKALYMDALAQPQGMILVTG
PTGSGKTVSLYTGLNILNKPERNISTAEDPVEINLEGINQVQINPKAEMTFANALKAFLRQDPDVVMVGEIRDLETAEIS
IKAAQTGHLVMSTLHTNSAPETLTRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPETLPEEELNRQGFVKAGINDI
TLYKANGCDHCTEGYKGRVGVYEVVKITPEIAQVIMEGGNSIQIADMAAKAGFANLRISGLKKAAAGVTSLAEINRVTSY

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=1070344 ACH784_RS14730 WP_404391446.1 3251772..3253454(+) (pilB) [Pseudoalteromonas phenolica strain a1]
ATGGAACATCACTCTCCCCTTTTACGGAAATTTATTACACTCGGTCGTGTGACCGCAGAGCAGCTAAAACTAAAACAAGC
CGAAGCGCAATCAACGGCAGAGCTGATCTGTTTGTGTAGTGGGATGACAGGTCAAGAACTTGCTGAACAATGCCTAGACC
TATTCAGAGTGCCAATGTTTGACCTAAGCGATTTTGACGTCGAACAACTGCCAAAAGAGCTTATTAAAGAAAAACTGATC
CGTCAGCACCATTTGCTCCCCCTTGTAAAAAAAGGCCGAAAGCTATTTATCGCCACCTCAGATCCCACTGATTATGGTGC
GTTTGAGAACTTCGAATTTAGCACAGGCCTGCAATGTGAAATCGTTGTCGTTGATTACAAACAATTAGAACAAAAGATAG
ATCAACTATTCGATGCCAATGGCGGCCTATCCATGAGTGAGGATGACTTTAAAGAATTCTCAGGCATGGATGTTGAAGAC
ACTCAACAGCAGCAAGACAGCAACGATGAAAAAGACAAAGATGATGCCCCTATCATTGTCTACATCAATAAGATTTTAAT
GGATGCGATTAAAAAAGGCGCGTCAGATTTACACTTTGAGCCTTACGAACATAAATACCGCATTCGTTTTCGTATCGATG
GCATGCTCCATGAAATGGCCAGTCCGCCAACCAGTTTATCAACGCGTTTATCGGCGCGTATCAAGGTTATGTCTCGCTTA
GACATTGCCGAAAAACGTAAACCGCAAGATGGCCGCATTAAATTAAAGATCTCAGATCGTAAAAGTATCGATTTTCGTGT
CAGTACCCTACCCACGTTATGGGGTGAGAAAATCGTAATGCGTATTCTAGACTCATCAAGCGCCATGTTAGGCATTGATG
TGTTGGGTTACGAGCCAGAGCAAAAAGCACTCTACATGGATGCACTGGCGCAGCCACAAGGCATGATCTTAGTAACCGGT
CCAACGGGTTCTGGTAAAACAGTATCACTTTATACGGGTTTAAATATTCTTAATAAACCTGAGCGTAACATCAGCACTGC
GGAAGATCCGGTTGAAATTAACCTTGAAGGGATCAACCAAGTTCAGATCAATCCAAAAGCAGAGATGACCTTTGCCAACG
CGCTTAAAGCCTTCTTACGTCAAGATCCTGATGTGGTGATGGTTGGTGAGATCCGTGACTTAGAAACCGCTGAGATCTCC
ATTAAGGCTGCACAAACGGGTCACTTGGTAATGAGTACACTTCACACCAACTCAGCACCAGAAACCTTAACCCGTCTATT
GAATATGGGTGTACCAGCTTATAACGTAGCAAGCTCGATCAGCTTGATCATCGCACAGCGTCTAGCGCGTCGTTTATGCC
CTAAATGTAAAACACCTGAAACCTTGCCTGAAGAAGAGCTCAATCGCCAAGGTTTTGTCAAAGCGGGGATCAACGACATA
ACACTATACAAAGCCAATGGCTGCGATCATTGTACTGAAGGCTACAAAGGCCGTGTTGGTGTATATGAAGTGGTTAAAAT
CACGCCTGAAATCGCACAAGTTATTATGGAAGGCGGTAACTCGATTCAAATCGCTGACATGGCAGCAAAAGCTGGATTTG
CGAATTTACGTATTTCAGGTCTGAAAAAAGCCGCAGCAGGCGTGACTTCTCTTGCCGAAATAAATCGAGTAACCAGTTAC
TAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-451)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.634

100

0.564
  pilB Acinetobacter baylyi ADP1

55.437

100

0.555
  pilB Vibrio cholerae strain A1552

54.137

99.286

0.538
  pilB Legionella pneumophila strain ERS1305867

53.704

96.429

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

53.03

94.286

0.5
  pilB Vibrio campbellii strain DS40M4

52.552

94.464

0.496
  pilF Neisseria gonorrhoeae MS11

48.571

100

0.486
  pilB/pilB1 Synechocystis sp. PCC 6803

37.881

100

0.421

Multiple sequence alignment